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Application of a modified method for stem cell isolation from lipoaspirates in a basic lab Sardjono, Caroline T.; Setiawan, Melina; Frisca, Frisca; Saputra, Virgi; Aniko, Gwendy; Sandra, Ferry
Medical Journal of Indonesia Vol 18, No 2 (2009): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (718.567 KB) | DOI: 10.13181/mji.v18i2.343

Abstract

Aim Lipoaspirate, a wasted by product from liposuction procedure recently has been shown to contain abundant mesenchymal stem cells (MSCs). MSCs have been studied in many research areas to regenerate many cell lineages including, myogenic, cardiomyogenic, and angiogenic lineages. The large quantity of MSCs in lipoaspirate, makes it an attractive source for stem cells used in research and clinical applications. A simplified method which is suitable to be performed in a basic laboratory will facilitate development of stem cell research in developing countries. Therefore the outcomes from this study are expected to encourage the progress of stem cell research in Indonesia.Methods Lipoaspirate was digested using collagenase type I, followed by a basic filtration method. Purification of MSCs was done by cell culture for 2-3 days followed by supernatant removal. To confirm the homogenous population of MSCs, an analysis using flowcytometry was performed based on the MSCs minimal criteria developed by Mesenchymal and Tissue Stem Cell Committee of the International Society of Cell Therapy.Resuts MSCs were able to be obtained at 16.41 ± 8.22 x 108 cells per 120 ml lipoaspirate. The cultured cells showed fibroblastic morphology which is characteristic for MSCs and were able to be purified from non-MSCs cells. This was confirmed by flowcytometry assay showing expression of CD105 and the absence of HLA-Class II, CD 45, CD 34, CD14, and CD19.Conclusions This study has shown that it was feasible to isolate messenchymal stem cell from human lipoaspirate. The procedure was practicable to be performed within a basic laboratory. (Med J Indones 2009; 18: 91-6)Keywords: mesenchymal stem cell, lipoaspirate, stem cell isolation technique
PREVALENSI OBESITAS SENTRAL BERDASARKAN LINGKAR PINGGANG PADA PENGEMUDI BUS ANTAR KOTA Frisca, Frisca; Karjadidjaja, Idawati; Santoso, Alexander Halim
Jurnal Muara Sains, Teknologi, Kedokteran dan Ilmu Kesehatan Vol 3, No 2 (2019): Jurnal Muara Sains, Teknologi, Kedokteran dan Ilmu Kesehatan
Publisher : Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jmstkik.v3i2.3911

Abstract

Central obesity has become a public health problem which is currently rapidly increasing throughout the world, including in developing countries like Indonesia. The World Health Organization states that central obesity is a major risk factor for various cardiometabolic diseases, such as diabetes and hypertension. Inter-city bus drivers are high-risk occupations of cardiometabolic disease, this is due to unhealthy lifestyles and work patterns such as high consumption of fatty foods, smoking habits, lack of physical activity, long driving times and irregular sleep patterns. This study aims to determine the prevalence of central obesity based on waist circumference in inter-city bus drivers. This research is a descriptive cross sectional study. Data obtained from measurements of body weight, height and waist circumference, as well as calculation of body mass index (BMI) on 176 subjects obtained through consecutive sampling method. Obesity is said if BMI ≥25 according to WHO criteria for Asia-Pacific and central obesity if waist circumference> 90 cm for men and> 80 cm for women. In this study all subjects were men with an average age of 42.45  10.50 years. Based on the calculation of BMI there are 54.3% of subjects obese. Through measurement of waist circumference there are 50.6% of subjects with central obesity. Providing education is very important so that subjects can make lifestyle changes for the better so as to prevent cardiometabolic disease in inter-city bus drivers. AbstrakObesitas sentral telah menjadi masalah kesehatan masyarakat yang saat ini sangat meningkat pesat di seluruh dunia, termasuk di negara berkembang seperti Indonesia. World Health Organization menyatakan bahwa obesitas sentral merupakan faktor risiko utama terjadinya berbagai penyakit kardiometabolik, seperti diabetes dan hipertensi. Pengemudi bus antar kota merupakan pekerjaan dengan risiko tinggi terkena penyakit kardiometabolik tersebut, hal ini disebabkan karena gaya hidup dan pola kerja yang tidak sehat seperti tingginya konsumsi makanan berlemak, kebiasaan merokok, kurangnya aktivitas fisik, durasi menyetir yang lama dan pola tidur yang tidak teratur. Penelitian ini bertujuan  untuk mengetahui prevalensi obesitas sentral berdasarkan lingkar pinggang pada pengemudi bus antar kota. Penelitian ini merupakan penelitian deskriptif dengan desain potong lintang. Data diperoleh dari pengukuran berat badan, tinggi badan dan lingkar pinggang, serta  perhitungan indeks massa tubuh (IMT) pada 176 subjek yang diperoleh melalui metode consecutive sampling. Dikatakan Obesitas jika  IMT ≥25 menurut kriteria WHO untuk Asia-Pasifik dan obesitas sentral jika lingkar pinggang >90 cm untuk laki-laki dan >80 cm untuk perempuan. Pada penelitian ini semua subjek adalah laki-laki dengan usia rata-rata 42.45 ± 10.50 tahun. Berdasarkan perhitungan IMT terdapat 54.3% subjek mengalami obesitas. Melalui pengukuran lingkar pinggang terdapat 50.6% subjek dengan obesitas sentral. Pemberian edukasi sangat penting agar subjek dapat melakukan perubahan gaya hidup menjadi lebih baik sehingga dapat mencegah terjadinya penyakit kardiometabolik pada pengemudi bus antar kota.