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Efi Taufik
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Keefektifan Trichoderma viride TNU dalam Menghambat Infeksi Phytophthora palmivora pada Kakao Samsudin Samsudin; Rita Harni; Efi Taufik
Jurnal Tanaman Industri dan Penyegar Vol 5, No 1 (2018): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v5n1.2018.p39-48

Abstract

Phytophthora palmivora is a pathogenic fungus that causes pod rot and stem cancer in cacao plant. This pathogen was difficult to control because it survives in the form of mycelium and chlamydospores in infected plant parts or in soil. Trichoderma viride is expected to inhibit the growth and development of this pathogen. The study aimed to determine the effectiveness of T. viride in inhibiting P. palmivora infection on cacao, conducted at Plant Protection Laboratory and Greenhouse of Indonesian Industrial and Beverage Crops Research Institute (IIBCRI), Sukabumi from March to December 2014. The T. viride TNU isolates used was purified and propagated in the laboratory. The T. viride inhibition against  P. palmivora growth and development was tested in vitro on potato dextrose agar medium (PDA) and in vivo on infected cacao pods and seedlings. The parameters observed were percentage of inhibition on PDA and the disease progression on infected pods and seedlings. The results showed that T. viride inhibited the growth of P. palmivora with inhibition percentage up to 68.60%, a strong antagonist for P. palmivora on PDA and reduced P. palmivora infection on seedlings in the greenhouse. Applications of T. viride 3 days before or after inoculation with P. palmivora was able to protect cacao seedlings in polybags, respectively by 60% and 45%. However, T. viride has not been able to hinder the development of pod rot disease on cacao.
Pengaruh Minyak dan Ekstrak Tanaman terhadap Perkecambahan Uredospora dan Intensitas Serangan Hemileia vastatrix Rita Harni; Efi Taufik; Samsudin Samsudin
Jurnal Tanaman Industri dan Penyegar Vol 5, No 2 (2018): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v5n2.2018.p67-76

Abstract

Rust disease caused by fungus Hemileia vastatrix is a major disease of Arabica coffee, which reduces yield by 20%–70%. Botanical fungicide is a potential alternative because environmentally friendly and safe to humans health. The research aimed to analyze the effect of oils and extracts of fungicidal plants on uredospore germination and attack intensity of H. vastatrix. The research was conducted in laboratory and greenhouse of Indonesian Industrial and Beverages Crops Research Institute (IIBCRI), Sukabumi, from January to December 2016. The plant oils were of cloves, citronella, Reutealis trisperma, and neem, while the plant extracts used were mahogany, Ageratum conyzoides, and wood vinegar. Those oils and extracts were assessed on uredospores germination of H. vastatrix, both in vitro and on coffee seedlings in the greenhouse. A complete randomized block design was used with 9 treatments and 5 replications. The oils and extracts at 5% concentration were applied on coffee leaves of 6 months old plants then inoculated with H. vastratrix uredospora simultaneously. Attack symptoms, incubation period, attack percentage and intensity were observed. The results showed that plant oils and extracts used in present study effectively reduced the uredospora germination of H. vastatrix. However, oils of neem and R. trisperma as well as A. conizoides extract are more potential to suppress H. vastatrix infection in coffee leaves in greenhouse and reduced attack intensity from 22.2% to 3.6%; 5.2%; and 7.6% with inhibitory level at 83.8%; 76.6%; and 65.8%, respectively. Therefore, they are considered as potential biocontrols for rust disease.
Co-Authors Rita Harni Rita Harni Samsudin Samsudin Samsudin Samsudin