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The Diversity of Grasshoppers in Liwa Botanical Garden Based on Time Capture Alita Nur Afdila; M. Kanedi; Nismah Nukmal; Sutyarso Sutyarso
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 1 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i1.8

Grasshopper is one of the diurnal cosmopolitan insect, the existence of grasshoppers is very influential for some other animal populations such as birds. It is still lack of information of diversity, distribution, population, and other basic biological aspects of grasshoppers at Kebun Raya Liwa (KRL). To monitor biodiversity, it is necessary to provide informations about the number of individuals, their functions, and roles in the habitat and ecosystem. Therefore it is necessary to conduct the research of these topics. This research was carried out during November to December 2019, at the Taman Hias Kebun Raya Liwa. Grasshoppers were cached in the morning from 06.00 AM to 09.00 AM, day from 10.00 AM to 02.00 PM, and afternoon from 03.00 PM to 06.00 PM . The grasshoppers was cached using a sweeping net by exploring the Taman Hias Kebun Raya Liwa. The caught grasshopper was identified at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Universitas Lampung. The result show that ten species of grasshopper were found at the Taman Hias Kebun Raya Liwa, i.e. Phaleoba antennata, Phaleoba fumosa, Phaleoba rustica, Atractomorpha crenulata, Atractomorpha sp., Phillium crurifolium, Phillium sp, Trilophidia annulata, Oxya chienensis, and Melanoplus differentialentialis. The index diversity of grasshopper at the Taman Hias Kebun Raya Liwa is categorized to moderate with H' = 2.223. The best time to catch grasshoppers is in the morning from 06.00 AM-09.00 AM and afternoon from 03:00 PM – 05:00 PM.

Aggressiveness and Libido of Male Mus musculus L. in Ethanol Extract of Red Ginger (Zingiber officinale Roxb. var. Rubrum) Nida Nurhanifah; Sutyarso Sutyarso; M. Kanedi; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.21

The residue of paraquat herbicides may cause human health problems. Paraquat causes the testosterone hormone to decline. Testosterone hormone affects aggressive and sexual behavior. It is caused by free radicals in the body. Free radicals can be non-reactive if in the body there are antioxidants. Red ginger could be used as an antioxidant because it has active phenolic compounds, such as arginine, gingerol, shogaol, zingerone, gingerdiol, and zingiber. Ginger could increase serum testosterone levels. The purpose of this research is to know the effect of red ginger ethanol extract on aggressiveness and libido of male mice induced by paraquat herbicide. This study used a Completely Randomized Design (RAL) consisting of six treatment groups with four replications each. Group P1 as control (aqua dest), P2 (paraquat at dose 20 mg/kg BM), P3, P4, P5 (paraquat with dose 20 mg/kg BM and red ginger extract at 200 mg/kg BM, 400 mg/kg BM, 600 mg/kg BM) and P6 (red ginger extract at 600mg/kg BM). Paraquat is given 2 times a week for 21 days and the red ginger extract is given daily for 35 days. The result of analysis with One-way ANOVA and continued BNT at 5% showed that giving of red ginger ethanol extract can increase aggressive behavior and libido of male mice induced paraquat herbicide.

Spinal Structure of Mice Fetus (Mus musculus L.) Treated by Acanthus ilicifolius L. Leaves Extract Tunggul Van Roy; Nuning Nurcahyani; Sutyarso Sutyarso; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.27

Jeruju had various chemical components. This study was conducted on the teratogenic test of jeruju leaf extract to determine abnormalities in fetal mice (Mus musculus L.). This study used a completely randomized design (CRD) method with 20 pregnant female mice divided into 4 groups: 1 group as a control and 3 groups treated with a dose of 3.75 mg/30grBB, 7.5 mg/30grBB, 15 mg/grBB, each with jeruju leaf extract consists of 5 repetitions. The results showed that the doses of 7.5 mg/30grBB and 15 mg/grBB significantly decreased fetal weight and length when compared with controls. This is suspected because there is an active substance in the jeruju leaf extract which is cytotoxic. Anatomically the administration of jeruju leaf extract does not cause abnormalities in the structure of the fetal spine of mice. This is due to the growing fetus taking priority from the many types of nutrients in the mother fluid so that the fetal bone can continue to grow and develop.

PENGARUH PEMBERIAN EKSTRAK ETANOL JAHE MERAH (Zingiber Officinale Roxb var Rubrum) TERHADAP KUANTITAS DAN KUALITAS SPERMATOZOA MENCIT JANTAN (Mus musculus L.) YANG DIINDUKSI PARAQUAT DIKLORIDA Dian Anggraini; Sutyarso Sutyarso; M. Kanedi; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 5 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v5i2.53

Paraquat is one of the chemicals that contributes to increasing pollution Indonesia. The careless use of paraquat can increase production of ROS (Reactive Oxygen Species), resulting in damage to a wide range of vital organs and reproductive system disorders such as infertility. ROS in the body can be captured by antioxidants. Red Ginger contains high antioxidants because there are active phenolic compounds such as gingerol, shagaol, zingeron, ginggerdiol, and zingibren which are proven to protect body cells from damage caused by ROS. This study was aimed to test the effectiveness of red ginger extract (Zingiber officinale Roxb. Var. Rubrum) on the quantity and quality of sperm in male mice (Mus musculus L.) induced by paraquat dichloride.This research uses a completely randomized design and is divided into six treatment groups with each four replications: K1 as the control group (only given H2O); K2 (paraquat induced a dose of 20 mg/kg Body Weight (BW) without the administration of the test substances); K3; K4; K5 (paraquat induced a dose of 20 mg/kg Body Weight (BW) and was given a red ginger extract at a dose of consecutive succession 6 mg / ml, 12 mg / ml, 18 mg / ml) and K6 (Only given a test material the red ginger extract as much as 18 mg / ml). Paraquat was given 2 times a week for 21 days and the red ginger extract was given for 35 days. The results of analysis by One-way ANOVA followed LSD at 5% significance level showed the ethanolic extract of red ginger can increase the sperm count, motility, viability and Morphology sperm of mice induced by the paraquat diklorida.

PENGARUH PEMBERIAN EKSTRAK ETANOL JAHE MERAH (Zingiber officinale Roxb var Rubrum) TERHADAP JUMLAH SPERMATOGENIK MENCIT (Mus musculus L.) YANG DIINDUKSI SIPROTERON ASETAT Nur Bebi Ulfah Irawati; Sutyarso Sutyarso; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 3 No. 2 (2016)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v3i2.80

Infertilitas merupakan kondisi yang umum ditemukan dan dapat disebabkan oleh faktor perempuan atau laki-laki, parameter kesuburan dapat dilihat melalui kemampuan spermatozoa yang dihasilkan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol jahe merah dalam meningkatkan jumlah spermatogenik mencit(Mus musculus L.) yang diinduksi siproteron asetat. Penelitian ini menggunakan 25 ekor mencit jantan yang dibagi secara acak menjadi 5 kelompok yaitu kontrol normal, dimana hanya akan diberi pakan dan aquades. Kontrol negatif, diberikan siproteron asetat 1,17mg/ml secara oral selama 7 hari. Kelompok P1, P2 dan P3 diinduksi siproteron asetat 1,17mg/ml secara oral selama 7 hari kemudian diberikan ekstrak etanol jahe merah dengan dosis P1: 6 mg/ml, P2: 12mg/ml, dan P3: 24mg/ml selama 28 hari. Parameter yang dihitung dan diamati pada penelitian ini adalah jumlah sel spermatogonium, sel spermatosit primer dan sel spermatid mencit jantan. Pengaruh ekstrak terhadap parameter dianalisis dengan Analysis of Varian (ANOVA). Hasil penelitian menunjukkan bahwa ekstrak etanol jahe merah yang diberikan pada dosis yang berbeda dapat meningkatkan jumlah sel spermatogonium, sel spermatosit primer dan sel spermatid mencit jantan yang diinduksi siproteron asetat.

EFEK EKSTRAK LADA HITAM (Piper nigrum L.) TERHADAP LIBIDO MENCIT (Mus musculus L.) JANTAN YANG BERBEDA UMUR Tia Wida Ekaputri Hz; M. Kanedi; Sutyarso Sutyarso; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 2 No. 1 (2014)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v2i1.100

Potensi Ekstrak Etanol Daun Pare (Momordica charantia L.) sebagai Alternatif Obat Penyembuh Luka pada Punggung Mencit Jantan (Mus musculus L.) Muhammad Pazry; Hendri Busman; Nuning Nurcahyani; Sutyarso Sutyarso
Jurnal Penelitian Pertanian Terapan Vol 17 No 2 (2017)
Publisher : Politeknik Negeri Lampung.

The leaves of bitter melon (Momordica charantia L.) contains flavonoid, tannin and saponin which plays a role in wound healing. The purpose of this study was to determine the effect of bitter melon leaves (M. charantia L.) ethanolic extract on wound healing the back injury on male mice. The parameters of this study were the length of wound closure and histopathological observations by looking at the level of epithelialization, the number of new blood vessels, and the number of inflammatory cells.This study was conducted in a complete randomized design. The mice were divided into five groups: [K (+)], [K (-)], [ P1], [P2], [P3], each group consist four mice. All of mice was wounded by cut on the back skin about 1,5 cm in length. The wound were treated every two times a day for 9 days, K(+) is given povidone iodine as a positive control, K(-) is given 95% ethanol as a negative control, P1 is given 50% ethanolic extract of bitter melon, P2 is given 75% ethanolic extract of bitter melon and P3 is given 100% ethanolic extract of bitter melon. On the 10th day, one of the mice from all of groups was taken to observe the histopathology of their skin. The results showed that the leaf extract of bitter melon has wound healing potential by accelerate the healing process of back skinâ€™s wound in mice and has best histopathology picture compared to controls. Keywords : Bitter Melon (Momordica charantia L.), Male Mice (Mus musculus L.), povidone iodine

UJI KOGNITIF MENCIT (Mus musculus L.) JANTAN BERBEDA UMUR YANG DIBERI EKSTRAK LADA HITAM (Piper nigrum L.) Monica Sapitri; M. Kanedi; Hendri Busman; Sutyarso Sutyarso
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 2 No. 2 (2014)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v2i2.117

Lada hitam adalah salah satu jenis rempah yang diketahui mengandung antioksidan. Antioksidan adalah zat yang dipercayadapat menghambat proses penuaan. Penuaan ditandai dengan menurunnya berbagai fungsi organ tubuh, termasuk otak sehingga berdampak pada menurunnya daya ingat. Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak lada hitam terhadap daya ingat mencit (Mus musculus L.).Empat puluh ekor mencit jantan, masing-masing 20 ekor berumur 4 bulan (muda) dan 20 ekor berumur 6 bulan (tua), diberi empat perlakuan yaitu : tanpa ekstrak sebagai kontrol (K), ekstrak air (A), ekstrak etanol (E), dan ekstrak campuran air-etanol (C). Data dianalisis menggunakan Sidik Ragam 2 Jalur (Two Ways ANOVA). Hasil penelitian menunjukkan bahwa mencit tua lebih cepat mengingat jalur dalam uji labirin, tetapi cendrung tidak berbeda dengan mencit muda dalam mengingat balok pijakan pada uji renang. Melalui uji labirin, daya ingat mencit muda dan tua yang diberi ekstrak lada hitam terbukti lebih baik dari pada yang tidak diberi. Sedangkan dalam uji renang, daya ingat mencit muda lebih baik pada yang diberi ekstrak air, sedangkan pada mencit tua hanya ekstrak campuran yang bisa membuat daya ingat lebih baik.

PENGARUH PEMBERIAN EKSTRAK ETANOL JAHE MERAH (Zingiber officinale Roxb. Var. Rubrum) TERHADAP SPERMATOZOA EPIDIDIMIS MENCIT (Mus musculus L.) YANG DIINDUKSI SIPROTERON ASETAT Pepti Aristiani; Sutyarso Sutyarso; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 4 No. 1 (2017)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v4i1.120

Infertilitas merupakan kondisi yang umum ditemukan di Indonesia. Jahe merah (Zingiber officinale Roxb. var. Rubrum) merupakan tumbuhan yang memiliki banyak khasiat di berbagai aspek. Jahe merah memiliki pengaruh yang baik sebagai antioksidan terhadap sel spermatogenik dan parameter sperma pada mencit (Mus musculus L.) jantan yang diinduksi siproteron asetat. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol jahe merah terhadap jumlah ,motilitas, morfologi, dan viabilitas spermatozoa. Penelitian ini menggunakan metode Rancangan Acak Lengkap, menggunakan 25 ekor mencit jantan yang dibagi dalam 5 kelompok yaitu Kontrol Normal, K- (diberikan siproteron asetat 1,17mg/ml selama 7 hari), P1, P2 dan P3 (diinduksi siproteron asetat 1,17 mg/ml selama 7 hari dan ekstrak etanol jahe merah dengan dosis berturut-turut 6mg/ml, 12mg/ml, 24mg/ml selama 28 hari). Data yang di peroleh dianalisi menggunakan Analysis of Varian ( ANOVA). Hasil penelitian ini menunjukan bahwa ekstrak etanol jahe merah 6mg/ml, 12mg/ml, dan 24mg/ml dapat meningkatkan jumlah, motilitas, viabilitas dan menurunkan morfologi abnormal spermatozoa yang diinduksi siproteron asetat.

HISTOLOGI TESTIS MENCIT (Mus musculus L.) MUDA DAN TUA YANG DIBERI EKSTRAK LADA HITAM (Piper nigrum L.) Septiya Reni; M. Kanedi; Nuning Nurcahyani; Sutyarso Sutyarso
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 1 No. 2 (2013)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v1i2.144

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