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All Journal Jurnal Penelitian Pertanian Terapan Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Tundjung Tripeni Handayani
Jurusan Biologi FMIPA Universitas Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Gibberellic Acid (GA3) and Potassium Nitrate (KNO3): The Influence and Interaction inThe Senescence Process in White Rosa Sp. Nur Jannah Cortesa; Martha Lulus Lande; Zulkifli Zulkifli; Tundjung Tripeni Handayani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.28

Abstract

The purpose of this study was to find out whether the combination of KNO3 and GA3 solutions were more effective than a single solution of KNO3 or GA3 in keeping the freshness of cut flowers. The research was conducted in Botanical Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences University of Lampung from November to December 2017. The experiment was conducted in 2 x 3 factorial experiment with factor A is GA3 solution with 3 concentration level: 0% w/v, 25% w/v, and 0.5% w/v. Factor B is KNO3 solution with 2 concentration levels: 0% w/v and 5% w/v. The parameters of this study were fresh weight, dry weight, relative water content, total chlorophyll content, total chlorophyll of leaves, and total carbohydrate content of rose cut flowers. Measurements were made 7 days after the immersion of cut flowers in KNO3 or GA3 solution, and mixed both. Homogeneity of variance and analysis of variance is determined at confidence level 5%. KNO3 simple effect at every level of GA3 concentration is determined by the F test at 5%. The results showed that there was no interaction between GA3 and KNO3 to fresh weight, dry weight, relative water content and chlorophyll content a, b and total white roses, but KNO3 reduced fresh weight of cut flowers by 48.15% and dry weight 58.60%. KNO3 increased the relative water content of white rose flowers by 6.85%. The results of the study concluded that the combination of GA3 and KNO3 is not effective to keep the freshness of white rose flowers cut.
PERTUMBUHAN PLANLET KRISAN (Dendranthema grandiflora Tzvelev) KULTIVAR PINK FIJI SETELAH PENAMBAHAN EKSTRAK TAUGE (Vigna radiata L.) PADA MEDIUM MURASHIGE DANSKOOG (MS) SECARA IN VITRO. Nalindri Impitasari; Endang Nurcahyani; Tundjung Tripeni Handayani; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 5 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v5i2.50

Abstract

Chrysanthemum (Dendranthema grandiflora Tzvelev) is one of the important ornamental plants in Indonesia and has high economic value . This plant is known as a producer of flowers with attractive shapes and colors . Seeing the magnitude of community interest and the potential utilization of chrysanthemum , causing this plant more and more developed and cultivated . This study aims to determine the concentration of optimum mungbean sprouts extract on the growth of chrysanthemum explants in vita . The addition of mungbean sprouts extracts (Vigna radiata L .) from concentration of 0% v/v , 2% v/v , 4% v/v , 6% v/v and 8% v/v on Murashige and Skoog(MS ) medium to growth eksplan Chrysanthemum (Dendranthema grandiflora Tzvelev ) Pink Fiji cultivars have been carried out in the tissue culture laboratory of Faculty of Mathematics and Natural Sciences , University of Lampung from November to December 2017 . This study used Completely Randomized Design (RAL ) 1 factor with 5 replications . Analysis of BNT variety and test is done at 5% level . The results showed that the extract from mungbean sprouts (Vigna radiata L . ) had no effect on plantlet height , number of shoot and number of chrysanthemum (Dendranthema grandiflora Tzvelev) plantlet leaves. The addition of mungbean spourts extracts on Murashige and skoog (MS) medium show 100% live plantlet.
PENGARUH ASAM GIBERELAT (GA3) DAN KALIUM NITRAT (KNO3) SERTA INTERAKSINYA TERHADAP SENESCENCE BUNGA POTONG GERBERA PUTIH (Gerbera jamesonii). Maulidina Agustin; Martha Lulus Lande; Zulkifli Zulkifli; Tundjung Tripeni Handayani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 5 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v5i2.51

Abstract

The purpose of this study was to decide whether the mixture of KNO3 and GA3 solutions was more effective than a single solution of KNO3 or GA3 in maintaining the freshness of cut flowers. The study was conducted at the Botanical Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung from November - December 2017. The research was carried out in a 2 x 3 factorial experiment with reason A was the GA3 solution with 3 concentration levels: 0%, 0.5%, and 1%. Factor B is KNO3 solution with 2 concentration levels: 0% and 5%. The limits in this study were fresh weight, dry weight, relative water content, and total dissolved carbohydrate content of gerbera cut flowers, qualitative limits were the level of reducing sugars. Measurements were made 7 days after soaking cut flowers in KNO3 or GA3 solutions, and a mixture of both. Homogeneity of variance and analysis of variance were determined at a significant level of 5%. Simple effect of KNO3 at each concentration level of GA3 decide by F test at 5% significance level. The results showed that GA3 with a concentration of 0.25% and 0.5% increased the fresh weight and dry weight of Gerbera cut flowers. KNO3 0.5% much reduced the relative water content of Gerbera cut flowers. The interaction between GA3 and KNO3 does not much effect all freshness of cut flowers. From the results of the study concluded that the single treatment of GA3 was more effective than the combination treatment of GA3 and KNO3 in maintaining the freshness of Gerbera cut flowers.
METODE MENGHILANGKAN CAIRAN BUAH TOMAT DALAM UJI PERKECAMBAHAN BIJI TOMAT( Lycopersicum esculentum Mill.) Penta Santi Nabahan; Tundjung Tripeni Handayani; Martha Lulus Lande
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 1 No. 1 (2013)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v1i1.89

Abstract

Efek Alelopati Ekstrak Air Daun Mangga (Mangifera indica L. Var. Arumanis) Terhadap Pertumbuhan Rumput Teki (Cyperus rotundus L.) Davina Nathania Prasetya; Zulkifli Zulkifli; Tundjung Tripeni Handayani; Martha L. Lande
Jurnal Penelitian Pertanian Terapan Vol 18 No 3 (2018)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v18i3.1506

Abstract

The purpose of this research is to know whether the aqueous extract of mango leaves(Mangifera indica L.) affect the growth of purple nutsedge (Cyperus rotundus L.). Thisresearch conducted at Botanical Laboratory of Biology Department Faculty of Mathematicsand the Natural Sciences University of Lampung from September to October 2017. Thisexperiments were carried out in a complete randomized design with the main factors beingaqueous extract of mango leaves with 5 level concentration : 0% w / v ( control), 5% w / v,10% w / v, 15% w / v, and 20% w / v and consist of 5 replications. Parameters measuredwere shoot length, fresh weight, dry weight, and relative water content. The Levene testdetermined the homogeneity of variance at 5% significant level. Analysis of variance andTukey HSD testis did at 5% significant level. The results showed that the concentration ofextract was negatively correlated with shoot length where the shoot length was decreasedsignificantly at 20% extract concentration. Total fresh weight was decreased significantly at20% and correlated linearly negative with extract concentration. Total dry weight wasdecreased significantly at 20% extract concentration. There was no significant effect onrelative water content. From this research, it concluded that aqueous extract of mangoleaves at 20% extract concentration was decreased shoot length, fresh weight and dry weightof purple nutsedge.
UJI EFEKTIVITAS EKSTRAK DAUN CENGKEH (Syzygium aromaticum) SEBAGAI BAHAN DASAR OBAT NYAMUK ELEKTRIK CAIR TERHADAP NYAMUK Aedes aegypti Sasono Handito; Endah Setyaningrum; Tundjung Tripeni Handayani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 2 No. 2 (2014)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v2i2.118

Abstract

Aedes aegypti merupakan vektor penyakit Demam Berdarah Dengue (DBD) yang masih menjadi masalah kesehatan di Indonesia. Upaya pengendalian dan pemberantasan Ae. aegypti saat ini banyak dilakukan, salah satunya dengan menggunakan insektisida kimiawi. Penggunaan insektisida kimiawi secara berkelanjutan menimbulkan resistensi pada nyamuk vektor. Telah diketahui bahwa daun tanaman cengkeh (Syzygium aromaticum L) mengandung flavonoid dan saponin yang berpotensi sebagai insektisida. Untuk membuktikan hal tersebut dilakukan penelitian yang bertujuan untuk mengetahui efektifitas dari ekstrak daun cengkeh dengan menuntukan nilai Lethal Concentration (LC50 dan LC90) dan Lethal Time (LT50dan LT90). Penelitian eksperimen yang menggunakan rancangan acak kelompok ini dilakukan dengan lima konsentrasi, yaitu; 10%; 20%; 30%; 40%; dan 50% ekstrak daun cengkeh, dan dengan dua kontrol yaitu, kontrol negatif yang berisi aquades, kontrol positif yang berisi tranflutrin12,38g/l, dengan empat kali pengulangan setiap konsentrasi dan kontrol. Pengamatan terhadap jumlah nyamuk yang mati setiap 5, 10, 20, 40, 60, 120, 240, 480, dan 1440 menit setelah perlakuan. Dari hasil Uji ANOVA yang diuji lanjut dengan Uji BNT diketahui bahwa konsentrasi yang paling efektif dibandingkan kontrol negatif dan sama dengan kontrol positif adalah 50%. Dari analisis probit diperoleh nilai LC50 sebesar 43,709%, sedangkan untuk nilai LC90 sebesar 49,069%. Nilai LT50 dan LT90 dari penelitian ini adalah 1220,152 menit dan 1126,488 menit. Kesimpulan dari penelitian ini adalah ekstrak daun cengkeh berpotensi sebagai insektisida terhadap Ae. aegypti, dan konsentrasi ekstrak yang paling efektif adalah konsentrasi sebesar 50%.
PENGARUH ETHREL TERHADAP LAJU RESPIRASI DAN KANDUNGAN KLOROFIL PADA BUAH JERUK NIPIS (Citrus aurantifolia Swingle) SELAMA PEMATANGAN Ari Khusuma; Zulkifli Zulkifli; Tundjung Tripeni Handayani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 1 No. 2 (2013)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v1i2.140

Abstract

Pengaruh ethrel terhadap laju respirasi dan kandungan klorofil buah jeruk nipis (Citrus aurantifolia Swingle) selama pematangan telah diteliti pada bulan Maret 2013. Penelitian ini dilaksanakan dalam rancangan acak lengkap. Perlakuan yang digunakan adalah larutan ethrel 0%, 5%, 10%, 15% and 20% dengan 5 pengulangan. Parameter yang diamati adalah rata-rata laju respirasi dan kandungan klorofil 4 dan 8 hari setelah perlakuan. Laju respirasi diukur dengan respirometer sederhana. Kandungan klorofil diukur dengan spektorfotometer (645 dan 663 nm). Analisis ragam dan uji BNT dilakukan pada taraf 5%. Hubungan antara laju respirasi dan kandungan klorofil ditentukan dengan regresi. Hasil penetlitian menunjukkan bahwa ethrel dengan konsentrasi 15% meningkatkan laju respirasi 4 hari setelah perlakuan sedangkan konsentrasi 10% meningkatkan laju respirasi 8 hari setelah perlakuan. Konsentrasi ethrel 15% menurunkan klorofil a dan total 4 hari setelah perlakuan sedangkan konsentrasi 10% menurunkan klorofil a, b dan total 8 hari setelah perlakuan. Konsentrasi ethrel 15% merupakan konsentrasi optimal untuk meningkatkan laju respirasi dan kandungan klorofil. Dari percobaan ini disimpulkan bahwa ethrel mempengaruhi laju respirasi dan kandungan klorofil buah jeruk nipis.
ISOLASI DAN KARAKTERISASI AKTIVITAS ENZIM ALPHA-AMILASE PADA KECAMBAH KEDELAI PUTIH (Glycine max (L). Merill) DAN KACANG HIJAU (Phaseolus radiatus) DI BAWAH PENGARUH MEDAN MAGNET Widia Angraini; Sumardi Sumardi; Tundjung Tripeni Handayani; Rochmah Agustrina
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 1 No. 1 (2013)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v1i1.147

Abstract

Kedelai putih (Glycine max (L.) Merill) dan kacang hijau (Phaseolus radiatus) banyak dimanfaatkan sebagai bahan pangan karena kandungan protein dan karbohidratnya sangat tinggi. Saat berkecambah, karbohidrat dalam biji diurai menjadi senyawa yang lebih sederhana oleh enzim alpha-amilase. Salah satu faktor lingkungan yang mempengaruhi perkecambahan biji adalah medan magnet karena dapat mempercepat perkecambahan, sintesis protein, dan aktivitas di dalam sel. Tujuan penelitian ini adalah mengisolasi dan mengetahui karakter enzim alpha-amilase pada perkecambahan biji kedelai putih dan kacang hijau yang diberi perlakuan medan magnet. Penelitian dilaksanakan di Laboratorium Botani dan Laboratorium Biomolekuler Jurusan Biologi FMIPA Universitas Lampung dari bulan Mei sampai Juli 2012. Percobaan dilaksanakan menggunakan Rancangan Kelompok Teracak Lengkap dengan 3 ulangan. Perlakuan yang diberikan adalah lama pemaparan medan magnet 0,1 mT terdiri dari: 0 menit (kontrol), 7’48’’, 11’44’’ dan 15’36’’. Parameter yang diukur adalah aktivitas enzim alpha-amilase pada hipokotil dan kotiledon kecambah pada umur yang berbeda. Perbedaaan aktivitas enzim alpha-Amilase dianalisis berdasarkan rata-rata aktivitas enzim pada setiap perlakuan. Hasil penelitian menunjukkan bahwa medan magnet mempengaruhi aktivitas alpha-amilase selama perkecambahan. Lama pemaparan medan magnet yang baik untuk meningkatkan aktivitas alpha-amilase dan mempercepat perkecambahan kedelai putih adalah 15’36’’sedangkan untuk kacang hijau adalah 11’44’’ dan 15’36’’.
Co-Authors Agustrina, Rochmah Ari Khusuma Davina Nathania Prasetya Endah Setyaningrum Endang Nurcahyani Martha L. Lande Martha Lulus Lande Maulidina Agustin Nalindri Impitasari Nur Jannah Cortesa Penta Santi Nabahan Sasono Handito Sumardi, Sumardi Widia Angraini Yulianty Yulianty Zulkifli Zulkifli