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Journal : Jurnal Agroplasma

INDUKSI MUTASI PADA BUNGA MATAHARI (Helianthus annus L) MELALUI IRADIASI SINAR GAMMA Siti Hartati Yusida Saragih
JURNAL AGROPLASMA Vol 5, No 1 (2018): AGROPLASMA VOL 5 NO 1
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v5i1.1574

Abstract

Sunflower (Helianthus annus L.) is one of the ornamental plant which is economically. It is potential because of oil contains and has the beautiful flower. Induced mutation is important to get genetic diversity of Sunflower. Mutations are genetic changes in either a single cell or a number of genes or chromosomal changes that can cause somaclonal variations. This research was aimed to find the LD20 and LD50 and to identify genetic diversity of Sunflower with the dosage 0, 150, 200, 250 and 300 Gy. The results showed that the higher LD20 and LD50 was at 158.338 gy and 260.339 gy. The gamma ray iradiation was induce variation and affected significantly toward quantitative character of plant height. Key words: diversity, induced mutation, iradiation, LD20, LD50
EFEK LAMA PERENDAMAN TERHADAP PERKECAMBAHAN BENIH ROSELA (Hibiscus sabdariffa L.) Dede Suhendra; Yudi Triyanto; Siti Hartati Yusida Saragih; Zahlul Ikhsan
JURNAL AGROPLASMA Vol 6, No 2 (2019): AGROPLASMA VOL 6 NO 2
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v6i2.1567

Abstract

Rosela (Hibiscus sabdariffa L.) is a medicinal plant. Rosela has a chemical compound that can provide many benefits. Efficacy of rosella to prevent disease, treat disorders of various diseases with gossiptin anthocyanin content and gluciside hibiscin contained in it. This research was conducted at the Laboratory of College of Agricultural Sciences of Labuhan Batu University Foundation, North Sumatera Province. This research was started in March 2017. This research used Non-Factorial Randomized Block Design (RAK) with the harvesting period (P) P0: Control, P1: 1, Clock P2: 2 Hours, P3: 3 Hours P4: 4 Hours P5: 5 Hours P6: 6 Hours P: 7 Hours. The highest rate of germination was found in P7 treatment of 3.22 days. The highest normal germination percentage was found in P5 and P7 treatment of 66.67%. Than highest abnormal germination percentege was found in P3, P4 and P6 treatment of 33.33 %. Keywords : Rosela, Old Immersion
PERTUMBUHAN TANAMAN SAWI (Brassica juncea L.) DENGAN PERENDAMAN KOLKISIN Kamsia Dorliana Sitanggang; Siti Hartati Yusida Saragih; MHD Fadillah
JURNAL AGROPLASMA Vol 8, No 1 (2021): AGROPLASMA VOL 8 NO 1
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v8i1.1958

Abstract

Brassica juncea is one of vegetable consumed by many people. One of the ways to expand genetic diversity is by inducing the occurrence of mutations. Mutations can be induced by offering chemical mutagens as well as physical mutagens. One of chemical have been successful to induce mutation is colchicine. The aim of this research was to determine effect of colchicine on Brassica growth. This research was conducted in June 2019 in the Experimental Field, Labuhanbatu University. The result show that soaking brassica seeds using colchicine make a decrease in the average plant height, leaf widht and number of leaves compare to control cause abnormal cell division. Keywords: Brassica juncea, Colchicine, Growth response
Efek Iradiasi Sinar Gamma Pada Tanaman Kacang Penutup Tanah (Mucuna bracteata L.) Siti Hartati Yusida Saragih
JURNAL AGROPLASMA Vol 8, No 1 (2021): AGROPLASMA VOL 8 NO 1
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v8i1.1955

Abstract

Legume cover crop (Mucuna bracteata L.) is a creeper which is currently often used to increase soil fertility in plantation areas. This plant is a leguminous plant that can fix nitrogen nutrients in the soil. One of the M.bracteata plant breeding programs to increase diversity is mutation using gamma ray radiation. The research objective was to determine the level of radiosensitivity of legume cover crop using gamma ray irradiation. The research was conducted at PAIR BATAN using a Gamma Chamber 4000A irradiator and in agricultural experiment, Labuhanbatu University. The plant material used was M.bracteata seeds with irradiation doses of 0, 200, 250, 300, 350 and 400 Gy. This study uses a curve fit analysis program to calculate the LD50 value (Lethal dose 50). The results showed that the plant radiosensitivity by calculating the LD50 value of M.bracteata at the age of 2 Weeks After Planting) was 348.737 Gy. The higher the radiation dose given, the lower the percentage of growth. Keywords: diversity; leguminous; lethal dosage; mutation; gamma rays
PENGARUH FOTOAUTOTROFIK TERHADAP PERTUMBUHAN TUNAS KRISAN DALAM PROSES KULTUR IN VITRO SERTA PERBEDAAN STOMATA INVITRO DAN EXVITRO KRISAN Siti Hartati Yusida Saragih; Khairul Rizal; Kamsia Dorliana Sitanggang
JURNAL AGROPLASMA Vol 7, No 1 (2020): AGROPLASMA VOL 7 NO 1
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v7i1.1687

Abstract

Chrysanthemum (Chrysanthemum indicum L.) is an ornamental plant that is included in an important contribution in the ornamental plant business. Related to chrysanthemum buds grown in vitro in culture tubes or bottles with a tightly closed to avoid bacterial and fungal contamination and to protect the humidity of the culture environment. However, this tight cap often affects the composition of the gas in a jar or bottle, which inhibits plant growth. The purpose of this study was to study the effect of photoautotrophic on the growth of chrysanthemum buds in the in vitro culture process and the differences in invitro and exvitro chrysanthemum stomata. This research was conducted in March 2016 to May 2016 in the Microtechnical Laboratory, IPB University. Research results obtained from research on the position given to research conducted on the number of roots, the number of books and the number of shoots. The influence of the environment of exvitro also significantly affects the number of stomata, stomata width and stomata density. Keywords :ex vitro,  in vitro, photoautotrophic