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H. Harapan
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Biochemistry, Genetics & Molecular Biology Engineering Immunology & microbiology Materials Science & Nanotechnology Other

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Effect of rubella vaccine to plasmodium-infected mice parasitemia levels Jonny K. Fajar; H. Harapan; Kurnia F. Jamil; Tjut M. Zanaria
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Malaria, an infectious disease causes by Plasmodium, contribute to 300-900 million morbidities and 1-3 million mortalities, annually. This study aimed to determine the Rubella vaccine potency to inhibit Plasmodium berghei merozoite invasion to erythrocyte which evaluated from parasitemia levels, mortality and mice clinical condition. This study conducted in mice injected Rubella vaccine prior injected with P. berghei. There were four groups: A, B and C injected with 500 µl, 2 µl, and 1 µl rubella vaccine respectively. Group D injected with 500 µl sterile aqua, as control group. After 28 days of vaccination all mice injected with 0.2 ml P. berghei. Furthermore, we observed to parasitemia levels, death, and clinical condition of mice to assess Rubella vaccine effectiveness to inhibit merozoite invasion. The results shown Rubella vaccine did not provide significant effect on parasitemia levels. It might Rubella vaccine dose we used under effective dose and less effective of target site. However, our study shown Rubella vaccine provided significant effect on mice mortality (p0.05). It is probably due to Rubella antigen (213-239 amino acid sequence) and malaria antigen merozoite surface protein (MSP)-119 (sequence amino acid 238) has similar structure, thus it was likely generated cross-immunity. In conclusion, 28 days Rubella vaccination did not provide significant effect on parasitemia levels, however it provided significant effect on mice mortality with infected by P. berghei.
Molecular pathogenesis of preeclampsia: microRNA hypothesis Mohd. Andalas; H. Harapan; Diky Mudhakir; Muhammad Ichsan; Natalia C. Pedroza; Saurabh Laddha
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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The discovery of micro RNA (miRNA) in 1993 by Ambros and colleagues has a huge influence in pathogenesis theory, diagnosis and treatment approach of some diseases. Some studies have conducted to seek the association alterations of miRNA expression to incidences and severity of preeclampsia (PE). We have reviewed some studies that conducted to seek the association of miRNA and PE and we discussed the role of various miRNAs in PE pathogenesis. In summary, we have shown that many researchers have given evident that the different placental and plasma miRNA expression is associated with PE. Some studies also identified the novel candidate of miRNAs (and their pathways) that may be of etiologic relevance in the pathogenesis of PE. Base on review, specific miRNA have a role to down regulate of anti apoptosis genes, regulate angiogenics growth factors such as angiogenin, vascular endothelial growth factor (VEGF) B (VEGF-β), cysteine-rich 61 (CYR61), Placental growth factor (PlGF) and VEGF-A that have a role in angiogenesis. miRNA also have a role in  survival, migration, and capillary tube formation of HUVEC by targeted of c-kit. Some miRNAs target genes that participate in immunologic dysfunction, cell adhesion, cell cycle, and signaling. miRNA also have a roles in endothelial cell response to hypoxia, cell differentiation, and survival. A miRNA influence calcium signaling through negative regulations of the calmodulin-coding mRNAs, Mef2a and Gata4, mainly in smooth muscle cells that contribute to PE pathogenesis. These investigations provide novel targets for further investigation of the pathogenesis of PE and these differential miRNAs may be potential markers for the diagnosis and provide a potential therapeutic target for PE. Further investigations on posttranscriptional regulation in PE to evaluate biologic effects of identified miRNAs (including confirmations of miRNA and target gene interactions) are needed
Factors affecting the level of health care worker’s stigmatized and discriminatory attitude towards people living with HIV: A study at the Dr. Zainoel Abidin General Hospital, Banda Aceh, Indonesia H. Harapan; Syarifah Feramuhawan; Hendra Kurniawan; Mohd. Andalas; Mohammad Bellal Hossain
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The aim of this study was to identify the level of health care worker`s (HCW) stigmatized and discriminatory (SD) attitude towards people living with HIV (PLHIV) and the factors that influenced this attitude. This research was conducted at the Dr. Zainoel Abidin General Hospital (RSUDZA) in Banda Aceh province of Indonesia. A cross-sectional study design was adopted for this research. Eighty nine HCWs were included in this study and they were selected purposively. Pearson correlation analysis, analysis of variance or independent sample t test analysis was used according to the type of data. We found that the level of SD attitude towards PLHIV in RSUDZA is high. Based on the Least Significant Difference Test, the average score of the answers between nurses and general practitioners and between nurses and medical students was significantly different(p=0.05). Pearson correlation analysis showed that the levels of SD attitude towards PLHIV was positively correlated with age (r=0.219, p=0.04) and irrational fear of HIV transmission (r=0.352, p=0.001) and negatively correlated with knowledge about HIV/AIDS (r=-0.336, p=0.001). Pearson correlation analysis also found that knowledge of HIV/AIDS negatively correlated with the irrational fear of HIV transmission (r=0.382, p=0.000). In addition, the level of SD attitude towards PLHIV was also associated with marital status of HCWs (p=0.020). Gender, education level, religion and the importance of religion in HCW lives wasn`t significantly affect to the level of SD attitude towards PLHIV in HCW. We concluded that the factors that influence the level of SD attitude towards PLHIV among the HCW are age, marital status, knowledge of HIV/AIDS, irrational fear of HIV transmission and HCWs occupations. To reduce SD attitude towards PLHIV among the HCWs, we recommend introducing some program to increase knowledge of HIV/AIDS
Identification of Thymocyte Subset by Multicolor Flow Cytometry ED LSR II FACSDriva - FlowJo Software Analysis H. Harapan; W. Wienands; I. Ichsan; Ana Indrayati
Indonesian Journal of Biotechnology Vol 13, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.242 KB) | DOI: 10.22146/ijbiotech.7794

Abstract

In their development, thymocytes express different cell surface molecules that important for identification of thymocyte subset. It’s not easy to detect this cell surface molecules to determine the thymocyte subpopulation for research. Here we used multicolor flow cytometry ED LSR II FACSDriva - FlowJo software to identify of thymocyte subset from thymocyte sample solution using several antibodies such as mouse anti rat CD2-FITC, mouse anti rat CD45RC-PE, mouse anti rat CD4-APC, mouse anti rat CD8á-PerCP, mouse anti rat CD3-Biotin + PE-Cy7 or APC-Cy7. We determined double negative and single positive thymocyt subset (CD4 or CD8), found that the double negative thymocyte subset express CD2 and CD45RC. It was useful to determine the thymocyte subset using multicolor flow sitometry ED LSR II FACSDriva - FlowJo software.
Co-Authors Ana Indrayati Diky Mudhakir Hendra Kurniawan I. Ichsan Jonny K. Fajar Kurnia F. Jamil Mohammad Bellal Hossain Mohd. Andalas Muhammad Ichsan Natalia C. Pedroza Saurabh Laddha Syarifah Feramuhawan Tjut M. Zanaria W. Wienands