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All Journal Agroscientiae Journal of Tropical Horticulture Daun: Jurnal Ilmiah Pertanian dan Kehutanan Jurnal Hexagro AGROSCRIPT Journal of Applied Agricultural Sciences Agroekotek View JOURNAL OF WETLANDS ENVIRONMENTAL MANAGEMENT
Nofia Hardarani
Fakultas Pertanian Universitas Lambung Mangkurat Jl. Jend. A. Yani KM. 36 PO BOX 1028 Banjarbaru 70714, Kalimantan Selatan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science

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Journal : Daun: Jurnal Ilmiah Pertanian dan Kehutanan

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Efektivitas Formulasi Sterilan terhadap Jenis Eksplan pada Kultur Durian Lahung (Durio dulcis): Effectiveness of Sterilant Formulation on Type of Explant in Durian Lahung Tissue Culture Nofia Hardarani; Chatimatun Nisa
Daun: Jurnal Ilmiah Pertanian dan Kehutanan Vol. 9 No. 2 (2022): Daun: Jurnal Ilmiah Pertanian dan Kehutanan
Publisher : ​Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/daun.v9i2.4264

Abstract

Durian lahung is a type of endemic durian to Kalimantan which has a unique characteristic, namely its red skin. The existence of this plant is almost extinct so that conservation needs to be done and one way is to use tissue culture techniques. As first step, it is necessary to conduct experiments to obtain the right sterilization method in order to obtain aseptic planting material. This study aims to determine the effectiveness of sterilant formulation on type of explant for lahung durian culture. This study was designed using two factors factorial Completely Randomized Design (CRD) and was repeated four times. The first factor was formulation of sterilant which consists of five levels and the second factor was type of explant which consists of two levels, i.e leaf and node. The results showed that the combination of sterilant formulation tween 20, fungicide, bactericide, Bayclin 20%, alcohol 70%, HgCl2 0,05%, H2O2 17,6% with leaf explant produced the highest percentage of browning until 4 wap. There was no browning in all combinations of sterilant formulations with node explant. The sterilant formulation tween 20, fungicide, baktericide, alcohol 70% gave the longest time to appear contamination and browning, smaller percentage of contamination and greater percentage of live explants at 2 and 3 wap, while at 4 wap it was obtained from the sterilant formulation tween 20, fungicide, bactericide, Bayclin 20%, alcohol 70%, HgCl2 0,05%. Leaf explants had lower percentage of contamination and higher percentage of live explants than node explants.
Respon Eksplan Buku Durian Lahung (Durio dulcis) terhadap Konsentrasi BAP (Benzil Amino Purin) pada Media WPM (Woody Plant Medium): Response of Durian Lahung (Durio dulcis) Nodus Explant to BAP (Benzyl Amino Purine) Concentration on WPM (Woody Plant Medium) Nofia Hardarani; Siti Nor Zahra; Raihani Wahdah; Juharni Juharni
Daun: Jurnal Ilmiah Pertanian dan Kehutanan Vol. 10 No. 2 (2023): Daun: Jurnal Ilmiah Pertanian dan Kehutanan
Publisher : ​Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/daun.v10i2.6091

Abstract

Lahung is a genus of endemic Durio in Kalimantan which currently has vulnerable status and is rare. Therefore, ex-situ conservation of this plant has begun using tissue culture techniques. Several things that need to be considered in in vitro propagation are the right type of explant, planting mediun and PGR. Stem nodes are generally chosen as explant because their availability does not depend on the season and has a fast response in bud induction. WPM medium is e medium intended for woody plants such as durian lahung. BAP is a PGR from the cytokinin group which is quite avtive and the most widely used. This research aimed to determine the best response of the durian lahung stem node culture to the BAP concentration on WPM medium. This research was designed using a rancomized block design (RBD) with one factor in the form of BAP concentration consisting of six levels, i.e. 0.0, 0.5, 1.0, 1.5, 2.0 and 2.5 ppm. Each experimental unit was repeated four times and consisted of ten planting bottles for e total of 240 planting bottles. The variables observed were the percentage of live explant aged 1-8 WPP, callus growth time, percentage of explant forming callus aged 1-8 WAP, callus and texture callus, shoot growth time and number of shoots. The results showed that callus appeared in response of the durian lahung stem node to the BAP concentration treatments.
Co-Authors Alia Rahmi Chatimatun Nisa Eka Agustiningrum Fakhrur Razie Fakhrur Razie Hanisa Desy Ariani Hilda Susanti Indya Dewi Juharni Juharni Raihani Wahdah Riedha Kariena Rodinah Rodinah Samharinto Samharinto Saniah Saniah Siti Nor Zahra