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The Potency of Azotobacter spp.Isolated from Tidal Land of South Kalimantan to Produce Indole Acetic Acids (IAA) Fakhrur Razie; Anas Iswandi
Jurnal Ilmu Tanah dan Lingkungan Vol 7 No 1 (2005): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Tidal land area is considered as the most frigile ecosystems. To open this area as agricultural land has to be donecarefully. To reduce the risk of declining the environmental quality of tidal swa,,!p area due to the agricultural activity, lhearea should be managed properly and Wisely especially when using agrochemicals such as fertilizers and pesticides. Inrelation to this, the use of biofertilizer such as Azotobacter spp, a nitrogen fIXing bacterium and IAA producer, might beimportant thingfor this area.The aims of this experiments was to study the ability of Azotobacter isolated from rice' rhizophere grown in tidalswamp area of South Kalimantan, in producing IAA to stimulate the growth of roots of rice cultivar I R-64. The parametersused to evaluate the effect of Azotobacter inoculation were the increase of the surface area of root, length of roots, totallength of roots, fresh, dry weight of rice IR-64 roots and the growth of root hairs.The results showed that Azotobacter produced less IAA in the media containing Urea (18.28-35.54 ppm IAA) comparedto Azotobacter grown in media without Urea (33.89 - 42.01 ppm IAA). Azotobacter T.M. UNST.3 produced the highest IAA(42.01 ppm), therefore they were able to increase the surface of roots, increase the length and weight of roots of rice cultivarIR-64 compared to other Azotobacter strains. In media containing Urea, Azotobacter RG 3.62 produced the least IAA (/8.29 ppm IAA) compared to other Azotobacter strains. However, this particular Azotobacter strain was able to increase the surface area of root, increased the number of root hairs compared to other strains.

AKTIVITAS ENZIM SELULASE MIKROBA YANG DIISOLASI DARI JERAMI PADI DI PERSAWAHAN PASANG SURUT DI KALIMANTAN SELATAN Fakhrur Razie; Iswandi Anas; Atang Sutandi; Lukman Gunarto; Sugiyanta Sugiyanta
Jurnal Ilmu Tanah dan Lingkungan Vol 13 No 2 (2011): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Enzim selulase terdiri dari tiga enzim ekstraselular yang bekerja secara sinergis dalam mendegredasi selulosa, yakni endoglukanase, eksoglukanase dan β-glukosidase. Tiga enzim tersebut berperan dalam mendegradasi selulosa menjadi gula sederhana. Penelitian ini bertujuan untuk mengisolasi dan menyeleksi mikroba (bakteri dan fungi) berdasarkan aktivitas enzim selulase dari mikroba tanah yang diisolasi dari persawahan pasang surut Kalimantan Selatan. Kemampuan mengekskresikan enzim endoglukanase dinilai berdasarkan nilai indeks selulolitik pada media CMC dan kemampuan mengekskresikan enzim eksoglukanase dan β-glukosidase diukur dari aktivitas kedua enzim tersebut menggunakan metode Mandel yang dimodifikasi. Hasil yang diperoleh menunjukkan bahwa kisaran nilai indeks selulolitik dari mikroba selulolitik di persawahan pasang surut tipe A sebesar 2.29-3.72, di lahan tipe B sebesar 2.66-5.41, dan di lahan tipe C sebesar 1.84-3.34. Aktivitas eksoglukanase dari mikroba selulolitik di persawahan pasang surut tipe A sebesar 0.27-1.65 nkat mL-1, lahan tipe B sebesar 0.37-1.85 nkat mL-1, dan lahan tipe C sebesar 0.31-1.85 nkat mL-1. Mikroba selulolitik dari persawahan pasang surut Kalimantan Selatan memiliki aktivitas β-glukosidase sebesar 0.05-1.52 nkat mL-1. Isolat- isolat mikroba selulolitik yang memiliki aktivitas selulase tertinggi adalah isolat bakteri selulolitik J11, J42, R23, BK12, C52, TB41, B82 dan SN123, dan isolat fungi selulolitik ST33, ST22, TB31, B52, GA22, TD11, PI52 dan P31.

EFFECT OF AZOTOBACTER AND AZOSPIRILLUM ON GROWTH AND YIELD OF RICE GROWN ON TIDAL SWAMP RICE FIELD IN SOUTH KALIMANTAN Fakhrur Razie; Iswandi Anas
Jurnal Ilmu Tanah dan Lingkungan Vol 10 No 2 (2008): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Tidal swamp land is a potential area for rice cultivation. However, tidal swamp is fragile ecosystems, so that when this area is used for rice cultivation, it has to be done carefully. To reduce a risk of environmental pollution in the tidal swamp area due to rice cultivation, the area should be managed properly and wisely especially when using agrochemicals such as fertilizers and pesticides. In relation to this, the use of bio-fertilizer such as Azotobacter or/and Azospirillum, an atmospheric nitrogen fixing bacterium, might be an important thing for this area. The objectives of this study were to evaluate the number of NFB (i.e. Azotobacter and Azospirillum spp.) found in tidalswamp rice fields of South Kalimantan, and their ability in fixing atmospheric nitrogen and supplying this fixed nitrogen to rice, and in increasing rice yields. There were three research stages performed in this study. The first, collecting, isolating and purifying the NFB on the selective media of nitrogen free media. The second, a hydroponic experiment in the greenhouse using Yoshida nutrition solution (Yoshida et al., 1976 in IRRI, 2003) as culture media and selected Azotobacter and Azospirillum. The third experiment was to study the effect of selected Azotobacter isolates to increase growth and yield of IR64 and Margasari rice cultivars, and two Azotobacter isolates were combined with six inoculation methods for Siam Unus rice cultivar as treatments. The result showed that the number of NFB strains found in rhizospere rice cultivars were varied widely. Then, Azotobacter and Azospirillum spp selected from rice fields in the tidal land of South Kalimantan when associated with IR64 and Siam Unus rice cultivars have ability in fixing atmospheric N2 and in supplying N on the initial rice growth. Azotobacter T.B.PDST.2b and T.HM.BPMT.2b were significantly supplying N for early growth of IR64 rice cultivars (2.34 and 2.13 %N). The ability of these isolates to fix atmospheric N2 was similar to N fertilizer (urea) in supplying N (2.2% N). Only Azotobacter T.B.PDST.2b was relatively significant to supply N (1.36% N) for Siam Unus rice cultivar early growth, even though this strain has less ability than N fertilizer (1.94% N) in supplying N. Azotobacter isolates grow in media containing no urea were higher IAA produced than in media containing Urea. Furthermore, yield of IR64, Margasari and Siam Unus inoculated with Azotobacter isolates were 3.87–4.93; 4.63-5.36 and 5.44-6.42 ton/ha respectively. In conclusion, the utilization of Azotobacter and Azorpirillum spp to substitute N fertilizer would be able to increase effeciency of N nutritions and to avoid environment pollutions risks from agrochemical N fertilizer other than to increase rice yields on tidal land of South Kalimantan.Keywords: Azotobacter, Azospirillum, rice cultivars, tidal lands and rice yields.

PENGARUH KOMBINASI SEKAM PADI DAN BIOCHAR TANDAN KOSONG KELAPA SAWIT TERHADAP KETERSEDIAAN FOSFOR PADA BAHAN TANAH PERKEBUNAN KELAPA SAWIT Inna Maulida Rahmah; Indah Nirtha; Fakhrur Razie
Jernih: Jurnal Tugas Akhir Mahasiswa Vol 2 No 1 (2019): JERNIH
Publisher : Prodi Teknik Lingkungan Fakultas Teknik Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jernih.v2i1.583

Salah satu komoditas perkebunan yang berperan penting dalam menghasilkan devisa negara ialah perkebunan kelapa sawit. Perkebunan ini menghasilkan tanaman kelapa sawit (Elais guineensis Jacq) yang diproduksi menjadi minyak sawit. Akan tetapi perkebunan ini juga memberikan dampak negatif terhadap lingkungan. Tandan kosong kelapa sawit (TKKS) yang dihasilkan ialah salah satu limbah padat terbesar pada perkebunan ini. Berdasarkan studi terbaru, TKKS dapat diolah menjadi biochar melalui proses pirolisis biomasa. Beberapa sifat kimia tanah yang dapat ditingkatkan melalui teknologi biochar ialah pH, KTK, C-Organik, N-Total dan peningkatan P-tersedia (Sudjana, 2014). Tujuan dari penelitian ini ialah untuk menganalisis pengaruh penambahan kombinasi sekam padi dan biochar dalam perbaikan ketersediaan hara fosfor dan waktu kontak optimal terhadap perbaikan ketersediaan hara fosfor. Dalam penelitian ini terdapat 6 perlakuan yaitu: kontrol, kombinasi biochar dan sekam, biochar, sekam, TKKS, sekam TKKS dengan pengulangan sebanyak 4 kali dan inkubasi selama 15 dan 30 hari. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa penambahan kombinasi bahan segar dan biochar TKKS tidak berpengaruh secara nyata terhadap P total tetapi berpengaruh terhadap kandungan P larut bahan tanah setelah masa inkubasi. Sedangkan waktu inkubasi terbaik selama penelitian pemberian kombinasi bahan segar dan biochar TKKS yaitu pada inkubasi selama 15 hari

UJI KEMAMPUAN Bacillus sp. ASAL PERSAWAHAN KALIMANTAN SELATAN DALAM MEMACU PERTUMBUHAN TANAMAN PADI (Oryza sativa L.) Asniah Asniah; Noor Aidawati; Fakhrur Razie
Agroekotek View Vol 2, No 2 (2019)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/agtview.v2i2.734

PGPR is a group of active bacteria colonized plant roots and well developed that is rich in organic material. This research was conducted in the laboratory of plant pathology greenhouse in the Faculty of Agriculture University of gastric Mangkurat Banjarbaru, which funded in May–August 2018. From the experiment there are five treatment four times and repeats. The treatment is A: Bacillus isolate: Marabahan, B: Bacillus isolate Kandangan, C: Bacillus isolate, Barabai, D: isolate Bacillus-Banjarbaru pelaihari, E: control (no Bacillus). Results of the study showed rice was given the inoculation with Bacillus spp. shows the number of saplings, plant height, wet weight of the plantlets, productive plants and plant dry weight was higher if compared with rice not applicable isolate Bacillus spp. isolates showed these Bacillus spp. were able to spur the growth of the rice plant.

The Growth Performance of Micropropagated Talasbanana (Musa paradisiaca var. sapientum L) Seedlings Cultivated on Peatlands Fakhrur Razie; Chatimatun nisa; Nofia Hardarani
Journal of Wetlands Environmental Management Vol 5, No 1 (2017): January-June
Publisher : Center for Journal Management and Publication

ABSTRACTTalas banana (Musa paradisiaca var. sapientum L.) has a high economic value. However, because of slowly breeding, a quick way of propagation: i.e. in vitro is required.Peatlands inSouth Kalimantan are quite large and has potential to be used for planting area of Talas banana. The acclimatization of seedlings planted on the peatsrequires more fertilizers, considering the low nutrient content of the peats. This study aims to determine the chicken manure and nitrogen-phosphor-potassium (NPK) fertilizer on the growth of Talasbanana acclimatized in peatlands. This study applied a randomized block design (RBD) factorial: (1) the amount of chicken manure (K), namely: k1 = 5 t ha-1; k2 = 10 t ha-1; k3 = 15 t ha-1; k4 = 20 t ha-1, and (2) the amount of NPK fertilizers (P), namely: p1 = 200 kg ha-1; p2 = 250 kg ha-1; p3 = 300 kg ha-1. Therefore, 12 treatments. DITAMBAHKAN TERDAPAT 12 PERLAKUAN. All treatments have 3 replicates. The results of the experiment showed that there was no interaction between doses of chicken manure with NPK. The amount of chicken manure 5 t ha-1 resulted in the highest increment of plant height observed at 2nd, 4th, 6th, 8th, 10th and 12thweek after planting, increment of stem diameter, addition of leaf number, width and length of leaves.The results of experiment also revealed that the best increment of plant height observed at 8th week after planting and stem diameter observed at 4th week after planting was obtained by 250 kg ha-1of NPK fertilizer application. KOMBINASI TIDAK BERPENGARUHKey-word: propagation; in vitro; peatlands; chicken manure; Talas banana

KAJIAN FLOKULAN POLIMER ANIONIK DENGAN METODE JAR TEST UNTUK PEMANFAATAN LUMPUR CAIR PT AIR MINUM BANDARMASIH (PERSERODA) Nurhidayah Lailiani; Fakhrur Razie; Danang Biyatmoko; Emmy Lilimantik
EnviroScienteae Vol 19, No 3 (2023): ENVIROSCIENTEAE VOLUME 19 NOMOR 3, AGUSTUS 2023
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v19i3.17264

This study aims to identify the characteristics of the liquid sludge produced in IPA 1 A unit Yani PTAM Bandarmasih and analyze the effect of different doses of anionic polymer flocculants on the parameters of the quality standards of the Minister of Health Number 32 of 2017 for sanitary hygiene. This research was carried out using the experimental design experimental method in the Design Randomized Group consisting of 6 (six) treatments. Analysis of the data used includes identifying the characteristics of liquid sludge in situ. Analyzing the effect of different doses of anionic polymer by testing the jar test method with several combination treatments of anionic polymer flocculants on the decrease in parameters according to Permenkes Number 32 of 2017 will be analyzed using analysis of variance. Multiple regression equations to provide information about the relationship between the dependent variable and one or more independent variables used in the study. From the results of the study of Anionic Polymer Flocculants Using the Jar Test Method for Utilizing Liquid Sludge of PT Air Drinking Bandarmasih (Perseroda) it can be concluded that 1) Liquid sludge of PTAM Bandarmasih which has not been managed is still not suitable for sanitation hygiene water because there are several parameters whose values have not been meet the quality standards of Permenkes No. 32 of 2017, 2) After the addition of anionic polymer flocculants, the value of all parameters decreased, but what met the overall quality standard was a dose of 10 ppm.

Mineralisasi Nitrogen pada Komposisi Bahan Gambut dan Kotoran Ayam Sukmawati Sukmawati; Fakhrur Razie; Abdul Haris
Acta Solum Vol 1 No 1 (2022): November 2022
Publisher : Department of Soil, Faculty of Agriculture, Lambung Mangkurat University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/actasolum.v1i1.1387

Peat soils contain high concentrations of organic nitrogen, but in a form that is not available to plants. South Kalimantan has NH4+ in the range 4.51- 736.86 mg.kg-1 and NO3- content is in the range of 0.37-54.69 mg.kg-1. The low availability of N nutrients in peatlands is due to the slow nitrogen mineralization process and the very high mobility of N in the soil. This study aims to determine the effect of the composition of peat and chicken manure on nitrogen mineralization. This study used a single factor in completely randomized design (CRD) with 5 treatments and 4 replications. carried out in greenhouses and in the Soil Laboratory of the Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru. The treatment is the composition of peat and chicken manure, namely 100% peat material, 80% peat material + 20% chicken manure, 60% peat material + 40% chicken manure, 40% peat material + 60% chicken manure, 20% peat material + 80% chicken manure. The best composition for nitrogen mineralization is based on the high nitrate content, which is found in the composition of 80% peat soil + 20% chicken manure in the first month of incubation, and on the composition of 80% peat soil + 20% chicken manure in the second month. This composition is the best composition from a combination of peat material and chicken manure against nitrogen mineralization in the form of nitrates.

Hubungan Ketersediaan Fosfor dan Kelarutan Fe pada Tanah Sawah Sulfat Masam Muhammad Khalqi Alwi; Fakhrur Razie; Ahmad Kurnain
Acta Solum Vol 1 No 2 (2023): Maret 2023
Publisher : Department of Soil, Faculty of Agriculture, Lambung Mangkurat University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/actasolum.v1i2.1839

One of the prospective places for agricultural usage in acid sulfate rice fields, which are typically spread out in lowland areas along coastlines that occasionally undergo flooding. If handled effectively in accordance with the issues found in the land, this site can be developed as a productive agricultural region. Agricultural development on acid sulfate soils frequently encounters a number of issues, including (pH) acidic soil, high iron content, a lack of phosphate availability, and the presence of pyrite (FeS2). In this study, the relationship between P availability, Fe solubility, the Fe-P fraction, pH, Eh, and pyrite depth in acid sulfate paddy fields will be examined. Purposive sampling was used in field research utilizing the survey method in the acid sulphate rice fields of Barito Kuala Regency. This research was based on the distribution map of Fe solubility in Barito Kuala Regency. The results of this study indicate that there are three variables that have a significant effect on P availability: soluble Fe amd Fe-P fraction with the power regression form and a very strong relationship; and soil pH with a linear regression form and a strong relationship. Pyrite depth and soil Eh had no significant relationship with P availability in acid-sulfate lowland rice fields.

Pengaruh Pemberian Pupuk Guano dan Dolomit terhadap Ketersediaan Unsur Hara Posfor pada Tanah Podsolik Indah Apriliana Puspitasari; Meldia Septiana; Fakhrur Razie
Acta Solum Vol 2 No 1 (2023): November 2023
Publisher : Department of Soil, Faculty of Agriculture, Lambung Mangkurat University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/actasolum.v2i1.2273

Podzolic soils have various problems including acidity of the soil (pH) and low soil nutrient content. This study aims to determine the effect of guano and dolomite on the availability of P nutrients in podsolik soils, and to determine the best treatment. This study was a pot experiment using a completely randomized design (CRD) with one factor, namely (P1 = Dolomite 2 t ha-1); (P2 = Guano Fertilizer 0,5 t ha-1); (P3 Guano Fertilizer 0.5 t ha-1 and Dolomite 2 t ha-1); (P4 = Guano Fertilizer 1 t ha-1 and Dolomite 2 t ha-1); (P5 Guano Fertilizer 1.5 t ha-1 and Dolomite 2 t ha-1). Each treatment was repeated 4 times, so there were 24 experimental units. The results showed that the application of dolomite and guano fertilizers had an effect on the available-P, pH, Exchangable Ca and Exchangable Al. However, it has no effect on Mg-dd in Podzolic soil. The application of dolomite and guano fertilizer with a dosage of 0.5 t ha-1 guano fertilizer and 2 t ha-1 dolomite was the best result for the availability of P, Exchangable Ca and pH.

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