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Mustanir Mustanir
Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Immunology & microbiology Neuroscience Physics

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Bioassay on n-Hexane Extract of Leaves Cassia alata against Candida albicans Muhammad Bahi; Radilla Mutia; Mustanir Mustanir; Endang Lukitaningsih
Jurnal Natural Volume 14, Number 1, March 2014
Publisher : Universitas Syiah Kuala

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Abstract

The study of biological activity from n-hexane extract of leaves’ Cassia alata had been done against Candida albicans as bioindicator. Dried leaves of C. alata (850 g) were macerated into n-hexane to obtain a dark green crude extract (31.79 g). The n-hexane crude extract was then subjected to vacuum liquid chromatography (VLC) and eluted with n-hexane and ethyl acetate to give 4 sub-fractions, namely fraction A (7.7251 g), fraction B (1.9419 g), fraction C (1.3565 g) and fraction D (1.4737 g) respectively. Based on phytochemical analysis, both nhexane crude extract and chromatographic sub-fractions contained steroids as their secondary metabolite constituent. The antifungal activity was tested against C. albicans using the agar-disc diffusion method with three different concentrations (10, 30 and 50%). Fraction D showed medium antifungal activity at 10.6, 11.3 and 12.6 of diameter-inhibition zones (mm) respectively.
Co-Authors Endang Lukitaningsih Muhammad Bahi Radilla Mutia