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Yusnita yusnita
Universitas Lampung
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A MEDIUM CONTAINING COMMERCIAL FOLIAR FERTILIZER AND SOME ORGANIC ADDITIVES COULD SUBSTITUTE MS MEDIUM FOR IN VITRO GROWTH OF DENDROBIUM HYBRID SEEDLINGS Dwi Hapsoro Hapsoro; Vincentia Atika Septiana Septiana; Sri Ramadiana Ramadiana; Yusnita yusnita
Jurnal Floratek Vol 13, No 1 (2018): April 2018
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (724.101 KB)

Abstract

In an attempt to obtain a cost-effective and a simpler way of growing Dendrobium seedling in vitro, this study was conducted to investigate effects of basal media and organic additives on in vitro growth of Dendrobium seedlings.  The basal media used were that of half strength Murashige and Skoog (½ MS medium) and that containing foliar fertililizer 32:10:10 and trace elements (FT medium). Treatments were combinations of basal media (1/2 MS medium and FT medium) and different kinds of organic additive (tomato, potato, and mungbean sprout juice and banana homogenate). The experiment was conducted in a completely randomized design with three replicates, 5 culture vessels per replicate and 4 seedlings ca. 1.5 cm per vessel.  The experiment showed that FT medium resulted in significantly higher growth than ½ MS medium as indicated by seedling fresh weight.  The media containing tomato juice led to highest growth of seedling as shown by seedling fresh weight and seedling height. Since FT medium caused better growth than ½ MS medium, the FT medium could be used as a substitute for ½ MS medium for growing Dendrobium hybrid seedlings. The seedlings were successfully acclimatized in a shaded green house environment with 100% survival rate.
APPLICATION OF BENZYLADENINE (BA) IN THE FORM OF LANOLIN PASTE ON FLOWER-STALK BUDS SUCCESSFULLY INDUCED RE-BLOOMING OF HYBRID Phalaenopsis Mukhaila Iryani; Yusnita Yusnita; Dwi Hapsoro; Kukuh Setiawan; Agus Karyanto
Jurnal Agrotek Tropika Vol 8, No 2 (2020): JURNAL AGROTEK TROPIKA VOL 8, MEI 2020
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.641 KB) | DOI: 10.23960/jat.v8i2.3915

Abstract

Hybrid moth orchid (genus Phalaenopsis) is one of the most popular ornamentals in Indonesia. It has beautiful and long-lasting flowers, but cultivating this orchid is still become a challenging issues due to the need of specific condition to grow and long time period to re-blooming. Plant growth regulators (PGR) (i.e. Benzyladenine (BA)) has been widely documented as a flower-inducing substance in several orchids. However, the optimal concentration and its mechanism in inducing flower-stalk bud and re-blooming is still unclear. This research aimed to study the effects of BA application in the form of lanolin paste on hybrid Phalaenopsis flower-stalk buds. We conducted this study using completely randomized design with four replications at the greenhouse laboratory Faculty of Agriculture University of Lampung on August to December 2018. We divided the orchid into 5 group of BA concentration (0, 1000, 1500, 3000, or 6000 ppm). The sheath of the fourth or fifth buds of the flowers were carefully opened, then it smeared with BA. The percentage of bud break into flower spike or keiki, length of shoots or spike and number of open flowers were recorded until 10 weeks of observation. The results showed that, neither of the buds under the control treatment (without BA), 1000 ppm nor 1500 ppm BA broke and grew into keiki or spike. On the other hand, application of BA at 3000 ppm or 6000 ppm successfully induced 100% flower spikes on the buds treated. No keiki was formed in all buds treated. In addition, treatment of the buds with 6000 ppm BA produced longer flower spikes as well as more open flowers. We conclude that the application of BA (minimal 3000 ppm) successfully induced flower spike of  hybrid Phalaenopsis.
Resistance status and physiological responses of Dactyloctenium aegyptium to diuron herbicide in pineapple plantation RESTI PUSPA KARTIKA SARI; NANIK SRIYANI; YUSNITA YUSNITA; HIDAYAT PUJISISWANTO
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1457.772 KB) | DOI: 10.24815/jn.v22i3.25286

Abstract

Diuron herbicide has been used in the pineapple plantation in Lampung, Indonesia, for more than 35 years. It has been realized that the use of herbicides with the same mode of action intensively can speed up the evolution of resistant weeds over a long period of time. This study aimed to determine whether Dactyloctenium aegyptium from pineapple plantation has evolved resistance to diuron and to examine whether the resistance correlates with the weed physiological activities. The study was conducted at the University of Lampung, from September 2018 to March 2019. The study consisted of two stages, i.e. Stage 1: Weed resistance test and Stage 2: Physiological activity test on resistant weed. The study used a split-plot design. The main plot was the origins of weeds (exposed and unexposed to diuron) and thhe supplots was the diuron dose. The result showed that D. aegyptium exposed has high-level resistance to diuron. The physiological activities of D. aegyptium which has a high level of diuron resistance exhibited higher carbon assimilation, stomatal conductance, and transpiration rates than the sensitive D. aegyptium.
Resistance status and physiological responses of Dactyloctenium aegyptium to diuron herbicide in pineapple plantation RESTI PUSPA KARTIKA SARI; NANIK SRIYANI; YUSNITA YUSNITA; HIDAYAT PUJISISWANTO
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i3.25286

Abstract

Diuron herbicide has been used in the pineapple plantation in Lampung, Indonesia, for more than 35 years. It has been realized that the use of herbicides with the same mode of action intensively can speed up the evolution of resistant weeds over a long period of time. This study aimed to determine whether Dactyloctenium aegyptium from pineapple plantation has evolved resistance to diuron and to examine whether the resistance correlates with the weed physiological activities. The study was conducted at the University of Lampung, from September 2018 to March 2019. The study consisted of two stages, i.e. Stage 1: Weed resistance test and Stage 2: Physiological activity test on resistant weed. The study used a split-plot design. The main plot was the origins of weeds (exposed and unexposed to diuron) and thhe supplots was the diuron dose. The result showed that D. aegyptium exposed has high-level resistance to diuron. The physiological activities of D. aegyptium which has a high level of diuron resistance exhibited higher carbon assimilation, stomatal conductance, and transpiration rates than the sensitive D. aegyptium.
Co-Authors Agus Karyanto Dwi Hapsoro Dwi Hapsoro Hapsoro Hidayat Pujisiswanto Kukuh Setiawan Mukhaila Iryani Nanik Sriyani RESTI PUSPA KARTIKA SARI Sri Ramadiana Ramadiana Vincentia Atika Septiana Septiana