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Rahmi Fajri
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PENGARUH JENIS KAKAO DAN KOMBINASI ZAT PENGATUR TUMBUH TERHADAP INDUKSI EMBRIO SOMATIK SECARA IN VITRO Zuyasna Zuyasna; Erida Nurahmi; Rahmi Fajri
Jurnal Floratek Vol 9, No 2 (2014): Oktober 2014
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

In an effort to increase the productivity of cocoa in Aceh, the government has carried out a cocoa plantation revitalization program for plants aged 25-30 years. The revitalization effort was constrained by the unavailability of quality seedlings. Alternatively, cocoa seedlings can be made available through vegetative propagations or through tissue culture techniques. Based on those facts, we have studied the inducing of callus and somatic embryos of cacao clones that were adaptive and highly productive in Aceh. The experiment was arranged in a completely randomized design, consisted of two factors. The first factor was the cacao genotype, having red and green fruits skin. The second factor consisted of six combinations of growth regulators 2,4-D and kinetin. The results showed that genotype of explants origin from cocoa red flowers responded very well in the formation of callus and somatic embryo formation. There was a significant interaction between genotype and growth regulator combinations on the explants in the number of somatic embryos formed. The best combination of concentrations growth regulator in response to somatic embryo in SCG (Secondary Callus Growth) medium was 3 mgL-1 2,4 D and 1 mgL-1 kinetin for explants from cocoa red flowers, and 1 mgL-1 2,4 D and 0 mgL-1 kinetin for explants origin from cacao green flowers. 
The effect of picloram concentrations and explants types on the induction of somatic embryo on North Aceh Cocoa genotype . Zuyasna; Siti Hafsah; Rahmi Fajri; M. Ovan Syahputra; Gilang Ramadhan
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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.  The effect of picloram concentrations and three explants types on cacao somatic embryogenesis was studied. The objective of this work was to evaluate a procedure for somatic embryogenesis of cacao (Theobroma cacao L.) Aceh clones. Somatic embryogenesis was induced using floral tissue explants (petal, staminode, and anther) on North Aceh cacao genotype. No growth was observed on petal types of explants on low concentrations of picloram. Depending on the explants, the percentage of explants producing one or more embryos ranged from 3% to 75%, 5% to 80%, and 10% to 90% on media containing 3 mg/l, 4 mg/l, and 5 mg/l picloram respectively. Staminode explants produced 3 to 10 times more somatic embryos than petals, while anthers produced 2 to 5 times.  Secondary embryos were induced from the floral explants of the North Aceh cacao genotype that exhibited efficient primary embryogenesis. Percentage embryogenesis generally increased with culture time
Co-Authors . Zuyasna Erida Nurahmi Gilang Ramadhan M. Ovan Syahputra Siti Hafsah Zuyasna Zuyasna