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All Journal Natural Science: Journal of Science and Technology Jurnal Bios Logos Jurnal Planta Tropika Sustinere: Journal of Environment and Sustainability Biosaintifika: Journal of Biology & Biology Education

Sucipto Hariyanto

Department of Biology, Faculty of Science and Technology, Airlangga University, Campus C, Mulyorejo, Surabaya, Pos Code 60115, Indonesia

Author-ID : 276173

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Transportation

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Articles

The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro Utami, Edy Setiti Wida; Hariyanto, Sucipto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5165

Dendrobium antennatum has high economic value as cut flowers and flowerpots. Like orchid seeds in general, D. antennatum is difficult to germinate under natural conditions. This study aimed to determine the effect of coconut water on seed germination and embryo development, as well as the effect of NAA on shoots development of D. antennatum. This study consisted of two stages. In the first stage, the 12 weeks-old seeds after pollination were sown on MS medium containing 2 g/L peptone + 0%; 5%; 10%; and 20% coconut water. After 8 weeks of culture, the seeds germinated and the shoot formed were recorded. The highest in seed germination (92.2%) and the formation of shoots (51.4%) were obtained when seeds were cultured on MS medium containing 2 g/L peptone + 20% coconut water. In the second stage, the shoots were sub-cultured on MS medium containing 1 mg/L thidiazuron + 0 mg/L; 1 mg/L; 2 mg/L; and 3 mg/L NAA. After 16 weeks of sub-culture, the height of plantlets, the length of the roots and leaves, number of leaves and roots formed were recorded. MS medium containing 1 mg/L thidiazuron + 1 mg/L NAA was the most suitable for the shoots development of D.antennatum. The embryo development of D.antennatum in vitro begins with the enlargement of embryo, with further it emerges from the seed coat (germinated) followed by the formation of the apical meristems to form the shoots and the roots.How to CiteUtami, E. S. W., & Hariyanto, S. (2016). The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro. Biosaintifika: Journal of Biology & Biology Education, 8(2), 165-171.

Remediation of Pb(II) and Cd(II) in Sea Water by Skeletonema costatum Soedarti, Thin; Surtiningsih, Tini; Hariyanto, Sucipto; Kuncoro, Eko P.
Sustinere: Journal of Environment and Sustainability Vol 1 No 2 (2017): pp. 63 - 143 (December 2017)
Publisher : Centre for Science and Technology, IAIN Surakarta

The heavy metals that polluted sea water can end up polluting fish pond too. Hence, it is necessary to remove the heavy metals before sea water enters the fish pond. The aim of this study was to know the growth, remediation ability, and the highest of removal efficiency of marine diatom Skeletonema costatum in the lead (Pb(II)) and cadmium (Cd(II)) solution. This study used 3 x 4 factorial design, i.e. concentrations of the cell inoculation (5000 cells mL-1; 10000 cells mL-1; and 15000 cells mL-1) and concentrations of Pb(II) and Cd(II) (0; 0.5; 1; and 2 ppm), replicated five times. During five day exposure time, the cell density was observed daily using a microscope (400X), while filtrate of media was analyzed using AAS. The result showed that S. costatum could grow in Pb(II) and Cd(II) at 2 ppm, and could remediate Pb(II) and Cd(II) at 2 ppm. The highest removal efficiency of marine diatom S. costatum in Pb(II) solution was 80.5% (5000 cells mL-1 at 2 ppm on the first day), and in Cd(II) solution was 80% (15000 cells mL-1 at 0.5 ppm on the fifth day).

Vegetation and Community Structure of Mangrove in Bama Resort Baluran National Park Situbondo East Java Hariyanto, Sucipto; Fahmi, Akhmad Kharish; Soedarti, Thin; Suwarni, Emy Endah
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Ecotourism development program at Bama beaches area require baseline data of mangrove structure at Bama Resort and in the past two decades has been lost about 35% area of mangrove forest in Indonesia and in the worldThe aims of this study was to find structure, composition, distribution and zonation patterns of mangroves at Bama Resort Baluran Nasional Park. Ten belt-transects were laid perpendicular to the shoreline, using standard methods. Vegetation structure was determined using data collected on plant species diversity, density, basal area, and the number of each species of mangroves. Shannon Wiener index to calculated diversity, evennes and Simpson to calculated dominance index. The results show there are 2 families and 6 mangrove species occurring in the study areas that is Rhizophoraceae (Rhizophora stylosa, Rhizophora mucronata, Rhizophora apiculata, Bruguiera gymnorrhiza, and Ceriops tagal) and Araceae (Nypa fruticans). The highest importance value was R. apiculata (229.90%) for trees, R. apiculata (148.69%) for the sapling, and R. apiculata (244.83%) for the seedling. The diversity (H) and dominance index (C) values were moderate (1.79) and 0.521. The most dominant species was R. apiculata (C=0.487). The mangrove zonation pattern from coastline to the mainland was R. stylosa, R. mucronata, and R. apiculata, in the outer zone, respectively (zone directly adjacent to the sea); B. gymnorrhiza and C. tagal in the middle zone; and N. fruticans in the zone that adjacent to the mainland. The present study will aid in the conduct and preservation planning of mangrove forest especially at Bama coast and generally in the coastal areas of Indonesia.

DNA Barcoding: A Study of Guppy Fish (Poecilia reticulata) in East Java, Indonesia Hariyanto, Sucipto; Adro’i, Hasan; Ali, Mahrus; Irawan, Bambang
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Poecilia reticulata is a freshwater fish from the northeastern part of South America and spread widely to various countries in Asia and other continents. However, research about P. reticulate is limited even though it is a well-known fish species in Indonesia. The purpose of study was to identify the fish species of P. reticulata through DNA barcoding using the COI gene to determine the phylogenetic relationships among fish populations in East Java, Indonesia. In a present study, there were eight samples of P. reticulata from four different freshwater locations in East Java. Extraction, amplification, and sequencing of DNA samples were conducted to obtain the genetic data and construct a phylogenetic tree based on DNA sequences. The COI gene is the most popular markers to study genetic populations and phylogeography among the animal kingdom. Our phylogenetic reconstruction showed a clear that there were two groups of P. reticulata. The first group was obtain through species from East Java, Sukabumi, West Java (KU692776.1), Dominican Republic, Pandeglang, Banten and Myanmar. The second group was P. reticulata from southern Africa, Brazil, and Sukabumi, West Java (KU692775.1). The result of this study indicate that the guppy fish in East Java identic with P. reticulata from West Java (KU692776.1), which a widely used in classification based on evolutionary relationships. The findings of this study have important implication for the development of advance research about adaptation, phylogeny, and evolution of fish, especially of guppy fish.

INDUKSI REGENERASI PHALAENOPSIS AMABILIS (L.) BL PADA BERBAGAI DOSIS ZAT PENGATUR TUMBUH Utami, Edy Setiti Wida; Hariyanto, Sucipto
Natural Science: Journal of Science and Technology Vol 4, No 2 (2015): Volume 4 Number 2 (August 2015)
Publisher : Univ. Tadulako

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Penelitian bertujuan untuk mengetahui pengaruh 2iP, NAA dan kombinasinya untuk regenerasi anggrek P. amabilis. Potongan ruas dan buku-buku tangkai bunga dikultur dalam media New Phalaenopsis (NP) diberi zat pengatur tumbuh 2iP dan NAA dengan konsentrasi 0.0:0.0; 0.0:2.5; 0.5:2.0; 1.0:1.5; 1.5:1.0; 2.0:0.5 dan 2.5:0.0 mg L-1. Setiap 4 minggu, ruas dan buku-buku disubkultur pada media yang sama selama 12 minggu. Pengamatan terhadap waktu inisiasi kalus dilakukan setiap hari, waktu inisiasi tunas dan akar dilakukan setiap seminggu sekali. Penghitungan jumlah tunas dan akar dilakukan pada minggu ke 12 setelah inokulasi. Berdasarkan hasil penelitian dapat disimpulkan bahwa regenerasi tunas dari ruas tangkai bunga P. amabilis terbentuk secara tidak langsung melalui fase kalus. Regenerasi tunas dan akar dari buku-buku terbentuk tanpa melalui fase kalus. Perlakuan kombinasi zat pengatur tumbuh (2iP dan NAA) memberikan pengaruh yang lebih baik terhadap regenerasi daripada perlakuan zat pengatur tumbuh tunggal atau tanpa zat pengatur tumbuh. Buku-buku menghasilkan regenerasi tunas yang lebih tinggi daripada ruas.

Perkembangan Kultur Daun Aglaonema sp. dengan Perlakuan Kombinasi Zat Pengatur Tumbuh NAA dan 2,4-D dengan BAP (The Leaf Culture Development of Aglaonema sp. Treated by Combination of NAA, 2,4-D and BAP as Growth Regulators) Wahyuni, Dwi Kusuma; Prasetyo, Dedy; Hariyanto, Sucipto
JURNAL BIOS LOGOS Vol 4, No 1 (2014): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.4.1.2014.4837

Abstrak Tujuan dari penelitian ini adalah menentukan pengaruh kombinasi NAA dan 2,4-D dengan BAP terhadap perkembangan kultur daun dan konsentrasi yang sesuai untuk induksi kalus tiga kultivar Aglaonema sp. Kultivar-kultivar yang digunakan adalah Dynamic Ruby, Snow White, dan Siam Aurora. Eksplan daun dikulturkan pada medium MS padat dengan perlakuan kombinasi NAA dan 2,4-D dengan BAP. Hasil pengamatan minggu kedelapan menunjukkan ada pengaruh perlakuan zat pengatur tumbuh terhadap perubahan bentuk eksplan daun. Eksplan daun melengkung, bergelombang, membengkak, warna eksplan memucat dan membentuk kalus, tetapi hanya Aglaonema sp. cv. Dynamic Ruby yang mampu membentuk kalus. Kombinasi zat pengatur tumbuh 0,1 ppm 2,4-D and 1 ppm BAP adalah kombinasi yang sesuai untuk induksi kalus untuk Aglaonema sp. cv. Dynamic Ruby. Kata Kunci: Aglaonema, NAA, 2,4-D, BAP, kalus Â Abstract Â The objective of this study were to determine the effect of combination of NAA and 2,4-D with BAP toward leaf culture development and to know the best concentration of growth regulator substance to induce callus on leaf explant of three Aglaonema cultivars. The cultivars were Dynamic Ruby, Snow White, and Siam Aurora. Leaf explants were cultured on solid MS medium with addition of various concentration combination of NAA and 2,4-D with BAP. Result of eight week observation gave significantly effect to changes shape leaf. Leaf blade was curved or rolled up, swelled, leaf color became pale strands, and formed callus, but the only cultivar which formed callus was Aglaonema sp. cv. Dynamic Ruby. The conclusion of this study was combination of growth regulator substance 0,1 ppm 2,4-D and 1 ppm BAP are the appropriate combination on callus inducing for Aglaonema sp. cv. Dynamic Ruby. Keywords: Aglaonema, NAA, 2,4-D, BAP, callus

Effects of Plant Media And Fertilization on The Growth of Orchid Plant (dendrobium sylvanum rchb. F.) in Acclimatization Phase Hariyanto, Sucipto; Jamil, Ahmad Ridlwan; Purnobasuki, Hery
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 7, No 1 (2019)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2019.095.66-72

This study was to evaluate the effect of plant media variation, time of fertilization, and combination of both on the growth of orchid plant Dendrobium sylvanum Rchb. f. in acclimatization phase. This study was designed using 6x4 factorial experimentation. The first factor was the variation of plant media: charcoals, coconut husk, bagasse, mixing of charcoal and coconut husk, combination of charcoal and bagasse, combination of coconut husk and bagasse. The second factor was fertilization time: no fertilization, 7 day-fertilization, 14 day-fertilization, and 20 day-fertilization for three months. The data were analysed by using Two Way MANOVA (Î± = 5%) and Duncan test. Based on the results, there were differences between media variation and fertilization time on the increase of leaf length and width, stem diameter, and root length of Dendrobium orchid in acclimatization phase. Combination of bagasse and coconut husk media showed the best result on media treatment. The best of fertilization time was 14 days. Moreover, the best of interaction between media and fertilization time showed by the combination of bagasse and coconut husk with 14 days-fertilization.

Co-Authors Adro’i, Hasan Dedy Prasetyo Dwi Kusuma Wahyuni Edy Setiti Wida Utami Fahmi, Akhmad Kharish Hery Purnobasuki Jamil, Ahmad Ridlwan Kuncoro, Eko P. Maemonah, Maemonah Mahrus Ali Suwarni, Emy Endah Thin Soedarti Tini Surtiningsih

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