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All Journal WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Livestock and Animal Research Jurnal Kedokteran Hewan
Arie Febretrisiana
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Utilization of Assisted Hatching Techniques to Enhance Embryo Implantation Arie Febretrisiana; Fitra Aji Pamungkas
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 27, No 1 (2017): March 2017
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.329 KB) | DOI: 10.14334/wartazoa.v27i1.1412

Abstract

Gestation is the main goal for in vitro fertilization. The embryo that has been developed outside the body will be transferred directly into uterus leading to the process of hatching, implantation, and pregnancy. However, approximately 85% of embryos that have been transferred were failed to implant and it might be caused by hatching failure. Hatching is the process of releasing embryo from zona pellucida. If this process does not occur, it will cause pregnancy failure. Assisted hatching is a mechanism that dealing with thinning, slicing or artificially making holes in the zona pellucida to improve hatching. The process can be applied both in fresh or frozen embryos. This review describes various methods in assisted hatching such as enzymatic, chemical, mechanical, and laser beam as well as their advantages and disadvantages. Generally, some researches show that the technology of assisted hatching can improve the percentage of hatching and implantation of the embryo. However, in spite of the benefits, there are such weaknesses find in the zona pellucida of the embryo that has been manipulated such as toxic hazard medium, the risk of damage to the blastomeres or monozygotic twinning. Therefore, it is advisable to perform assisted hatching in certain cases that tends to face obstacles during the process of hatching such factors as age, embryo quality, the thickness of the zona pellucida and the number of failures in the in vitro fertilization program.
Comparison of reproductive performance of Gembrong goats and male Boerka goats Husnurrizal Husnurrizal; Daffa Gustia Putra Akbar; Tongku Nizwan Siregar; Hafizuddin Hafizuddin; Sri Wahyuni; Anwar Anwar; Arie Febretrisiana
Livestock and Animal Research Vol 21, No 1 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i1.63859

Abstract

Objective: This study aims to compare the reproductive performance of Boerka goats and Gembrong goats.Methods: The research was conducted at the Sei Putih Slaughter Goat Research Workshop in Deli Serdang, North Sumatra. In this study, male Boerka (n=3) and Gembrong (n=3) goats, aged 3 years and having the same average Body Condition Score (BCS) of 3.83±0.29, were used. Reproductive performance observed included scrotal circumference, libido score, and semen quality macroscopically (volume, color, odor, and consistency) and microscopically (concentration, motility, viability, and abnormalities). The data collected was tabulated and analyzed by t test.Results: Of all the parameters of reproductive performance examined, it is known that only the parameters of semen volume (ml) and sperm concentration (x106 cells/ml) showed a significant difference (P<0.05) between Gembrong goats vs. Boerka goats with values of 0.50±0.0 vs. 1.0±0.20 and 1.557±712 vs. 4.500±317.65, respectively, while the other parameters did not show a significant difference (P>0.05).Conclusions: It was concluded that the volume and concentration of Boerka goats were higher than Gembrong goats.
TINGKAT FERTILISASI OOSIT DOMBA DARI OVARIUM YANG DISIMPAN PADA SUHU DAN WAKTU YANG BERBEDA SECARA IN VITRO Arie Febretrisiana; Mohamad Agus Setiadi; Ni Wayan Kurniani Karja
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.468 KB) | DOI: 10.24815/jn.v%vi%i.2810

Abstract

Penelitian ini bertujuan mengetahui pengaruh suhu dan waktu penyimpanan ovarium terhadap tingkat fertilisasi oosit secara in vitro pada  domba. Ovarium dibawa dari rumah potong hewan (RPH) dalam medium NaCl fisiologis pada suhu yang berbeda yaitu 27-28° C, 36-37° C, dan 4° C. Oosit kemudian dikoleksi dari setiap kelompok berdasarkan waktu penyimpanan yang berbeda yaitu 2-4, 5-7, dan 8-10 jam setelah domba dipotong. Oosit dikoleksi dan dimaturasi secara in vitro dalam inkubator 5% CO2, 38,5°C selama 28 jam. Oosit kemudian difertilisasi ke dalam drop spermatozoa selama 14 jam dalam inkubator CO2 5%, 38,5 C. Tingkat fertilisasi dievaluasi berdasarkan jumlah pronukleus yang terbentuk. Tingkat fertilisasi oosit yang dikoleksi dari ovarium yang disimpan pada suhu 27-28° C tidak berbeda dengan tingkat fertilisasi oosit yang disimpan pada suhu 36-37° C dan pada suhu 4° C, 2-4 jam setelah kematian hewan (masing-masing 53; 66,66; dan 63%) (P0,05). Tingkat fertilisasi oosit mulai mengalami penurunan pada tiga kelompok perlakuan setelah ovarium disimpan selama 8-10 jam, tingkat fertilisasi oosit yang disimpan pada suhu 27-28° C; 36-37° C; dan suhu 4° C masing-masing berturut-turut sebesar 9,8; 22,22; dan 12,24%. Dari hasil penelitian disimpulkan bahwa penyimpanan ovarium pada suhu 27-28° C dan 36-37° C selama 5-7 jam dapat mempertahankan kompetensi oosit dibandingkan dengan penyimpanan pada suhu 4° C.
TINGKAT FERTILISASI OOSIT DOMBA DARI OVARIUM YANG DISIMPAN PADA SUHU DAN WAKTU YANG BERBEDA SECARA IN VITRO Arie Febretrisiana; Mohamad Agus Setiadi; Ni Wayan Kurniani Karja
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2810

Abstract

Penelitian ini bertujuan mengetahui pengaruh suhu dan waktu penyimpanan ovarium terhadap tingkat fertilisasi oosit secara in vitro pada  domba. Ovarium dibawa dari rumah potong hewan (RPH) dalam medium NaCl fisiologis pada suhu yang berbeda yaitu 27-28° C, 36-37° C, dan 4° C. Oosit kemudian dikoleksi dari setiap kelompok berdasarkan waktu penyimpanan yang berbeda yaitu 2-4, 5-7, dan 8-10 jam setelah domba dipotong. Oosit dikoleksi dan dimaturasi secara in vitro dalam inkubator 5% CO2, 38,5°C selama 28 jam. Oosit kemudian difertilisasi ke dalam drop spermatozoa selama 14 jam dalam inkubator CO2 5%, 38,5 C. Tingkat fertilisasi dievaluasi berdasarkan jumlah pronukleus yang terbentuk. Tingkat fertilisasi oosit yang dikoleksi dari ovarium yang disimpan pada suhu 27-28° C tidak berbeda dengan tingkat fertilisasi oosit yang disimpan pada suhu 36-37° C dan pada suhu 4° C, 2-4 jam setelah kematian hewan (masing-masing 53; 66,66; dan 63%) (P0,05). Tingkat fertilisasi oosit mulai mengalami penurunan pada tiga kelompok perlakuan setelah ovarium disimpan selama 8-10 jam, tingkat fertilisasi oosit yang disimpan pada suhu 27-28° C; 36-37° C; dan suhu 4° C masing-masing berturut-turut sebesar 9,8; 22,22; dan 12,24%. Dari hasil penelitian disimpulkan bahwa penyimpanan ovarium pada suhu 27-28° C dan 36-37° C selama 5-7 jam dapat mempertahankan kompetensi oosit dibandingkan dengan penyimpanan pada suhu 4° C.
Co-Authors Anwar Anwar Daffa Gustia Putra Akbar Fitra Aji Pamungkas Hafizuddin Hafizuddin Husnurrizal Husnurrizal M Agus Setiadi Ni Wayan Kurniani Karja Tongku Nizwan Siregar Wahyuni, Sri