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IDENTIFIKASI ANTIBODI SPESIFIK Toxoplasma gondii PADA WANITA DI KOMUNITAS PECINTA SUGAR GLIDER INDONESIA (KPSGI) KOTA MAKASSAR Dzikra Arwie; Rahmat Aryandi
Jurnal Kesehatan Panrita Husada Vol 4 No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (Stikes Panrita Husada Bulukumba)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37362/jkph.v4i1.180

This research is motivated by quite a lot of public interest in maintaining pet animals such as sugar gliders but the negative effects of these animals can cause zoonotic diseases such as toxoplasmosis caused by the parasite Toxoplasma gondii. However, the presence of Toxoplasma gondii specific antibodies against sugar glider lovers is not known with certainty. This study aims to identify specific Toxoplasma gondii antibodies in women in the Makassar Sugar Glider Indonesia (KPSGI) Lovers community. The study was a laboratory observation with qualitative analysis techniques with the research subjects being women in the sugar glider (KPSGI) lover community in Makassar. Of the 10 samples taken by purposive sampling technique, 40% of female samples were obtained in the body with Toxoplasma gondii specific antibodies.

UJI BIOAKTIVITAS EKSTRAK DAUN BIDARA BIDARA (ZIZIPHUS MAURITIANA LAM) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS Aan Yulianingsih; Dzikra Arwie
Jurnal Kesehatan Panrita Husada Vol 4 No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (Stikes Panrita Husada Bulukumba)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37362/jkph.v4i1.181

The background of this study is Staphylococcus aureus is an anaerobic facultative bacterium and one of the normal microflora in the mouth. However, if it is influenced by predisposing factors, it will become a pathogen. Bidara leaves have antimicrobial activity against Staphylococcus aureus, because Bidara leaves contain various compounds including alkaloids, flavonoids, steroids, tannins and essential oils. The purpose of this study was to determine how much inhibitory power produced by Bidara leaf extract on the growth of Staphylococcus aureus bacteria. This research method is a laboratory experimental research using the diffusion test (wells) method. The sample of this study was Staphylococcus aureus. Dilution of Bidara leaf extract consists of 5 concentrations including: 20%, 40%, 60%, 80%, and 100%. Chlorampheniocol is used for Positive control while Negative control uses aquadest. Based on observations show that the average diameter of inhibitory zone Bidara leaf extract at a concentration of 20% with a diameter of 10.5 mm (R), at a concentration of 60% and 80% with a diameter of 16 mm and 18 mm (I), at a concentration of 100% with a diameter of 24 mm (S). It can be concluded that the higher the concentration of Bidara leaf extract, the inhibition zone formed will increase. So Bidara leaves are good for use in treating infections caused by Staphylococcus aureus bacteria.

PENENTUAN KRITERIA PENILAIAN KESAN JUMLAH LEUKOSIT PADA PEMERIKSAAN APUSAN DARAH TEPI Dzikra Arwie; Islawati
Jurnal Kesehatan Panrita Husada Vol 3 No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (Stikes Panrita Husada Bulukumba)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37362/jkph.v3i2.188

Leukocytes or white blood cells have a characteristic characteristic of different cells. Determination of the impression of the number of leukocytes is determined in the number of cells in the field of view. While the number of viewable field cells expressed is still quite varied. The purpose of this study was to determine the number of leukocytes in the field of view and expressed the impression of a sufficient amount. This research was conducted at the Laboratory of Health Analyst Department Panrita Husada Bulukumba on 9 April 2017 to 14 July 2017. This type of research is a laboratory observation that aims to determine the criteria for assessing the impression of the number of leukocytes on a peripheral blood smear. Data analysis using statistical analysis is the average and standard deviations to determine the impression of the number of leukocytes and use 3 inspection zones. The results of this study obtained results in zone IV the number of leukocyte impressions said to be sufficient was 7-10, in zone V the number of leukocyte impressions said to be sufficient was 4-9, and in zone VI the number of leukocyte impressions said to be sufficient was 3-8.

Ekstrak Antosianin Dari Ubi Ungu (Ipomoea Batatas L.) Sebagai Pewarna Alami Pada Pemeriksaan Soil Transmitted Helminths (STH) Metode Natif (Direct Slide) Subakir Salnus; Dzikra Arwie; Zulfian Armah
Jurnal Kesehatan Panrita Husada Vol 6 No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (Stikes Panrita Husada Bulukumba)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37362/jkph.v6i2.649

Helminthiasis is a worm infestation caused by several different species of intestinal parasitic worms. This group of worms belongs to the category of Soil Transmitted Helminth (STH) because the process of developing eggs or larvae of these species requires soil to develop into an infective form. The simplest method of examining intestinal nematode worm eggs is the Native Method using 2% Eosin reagent in observing various elements of the examination on the preparations/preparations. Eosin itself has properties that are not easily biodegradable, and generates hazardous waste (toxic) and flammable so that alternative coloring is needed that is more environmentally friendly. Purple sweet potato has a high content of anthocyanin pigments and is more stable than the pigments of strawberries, red cabbage, perilla and other plants. Thus, purple sweet potato has been considered a good source of anthocyanins. This study aims to determine the use of anthocyanin extract from purple sweet potato as a natural dye substitute for eosin dye in the identification of STH in the native method. Parameters observed in the preparations were the quality of visual field contrast, color absorption on the surface of the parasite, and the level of clarity of the appearance of the parasite. The results showed that the use of anthocyanin extract from purple sweet potato at a concentration of 80% could color the eggs of STH worms which were found in positive samples of Ascaris lumbricoides seen in fertile (fertilized eggs) and infertile (unfertilized eggs). Therefore, the anthocyanin extract obtained from purple sweet potato has potential as an alternative dye to replace eosin.

PEMERIKSAAN PROTEIN URINE PADA IBU HAMIL DI DESA BALANG PESOANG KECAMATAN BULUKUMPA KABUPATEN BULUKUMBA Asriyani Ridwan; Dzikra Arwie
Jurnal ABDIMAS Panrita Vol 2 No 1 (2021): Jurnal Abdimas Panrita
Publisher : Stikes Panrita Husada Bulukumba

Kebutuhan protein ibu hamil lebih banyak semasa kehamilan dibandingkan dengan yang tidak hamil. Hal ini dikarenakan protein diperlukan untuk perkembangan badan ibu hamil dan janinnya. Protein urine adalah protein yang terdapat di dalam urine akibat dari penurunan fungsi ginjal.Protein yang larut dalam pemanasan akibat aktivitas yang berlebih dapat melewati glomelurus dan tubulus sehingga terbaca sebagai proteinuria. Tingginya kadar protein dalam urin ibu hamil dapat mengindikasikan terjadinya preeklamsia. Penyakit ini umumnya terjadi dalam trimester kedua kehamilan. Pemeriksaan protein urin dibutuhkan oleh ibu hamil bila dicurigai mengalami preeklampsi ringan atau berat, dari hasil pemeriksaan ini kita dapat memberikan asuhan kepada ibu hamil yang ditujukan untuk mencegah timbulnya masalah potensial yaitu terjadinya eklamsi. Penetapan kadar protein dalam urin biasanya dinyatakan berdasarkan timbulnya kekeruhan pada urin. Berdasarkan hasil pemeriksaan yang telah dilakukan pada 22 ibu hamil di dapatkan hasil pemeriksaan protein urine dengan kadar Normal sebanyak 11 orang (50 %) dan kadar protein dalam jumlah kecil 0,15 mg/dl sebanyak 11 orang (50 %). Dampak dilakukannya pengabdian masyarakat yaitu Ibu Hamil di Desa Balang Pesoang menjadi tahu Kadar Protein Urine mereka sehingga bisa menjadi acuan saat dibutuhkannya informasi Mengenai Protein urine mereka sebagai langkah awal dalam mencegah terjadinya Preeklamsia.

Identifikasi Telur Cacing Nematoda Usus Menggunakan Metode Sedimentasi Pada Sampel Kuku Petani Sawah Di Wilayah Kelurahan Tanete Kecamatan Bulukumpa Kabupaten Bulukumba Kiki Fatmasari; Dzikra arwie; Fatimah
Jurnal TLM Blood Smear Vol 1 No 1 (2020)
Publisher : Stikes Panrita Husada Bulukumba

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.808 KB)

Penelitian ini berlatar belakang Infeksi kecacingan merupakan suatu penyakit yang terjadi di usus sebagai investasi satu atau lebih cacing parasit usus yang terdiri dari golongan nematoda usus. Petani yang setiap hari kontak langsung dengan tanah, akan mempercepat penyebaran infeksi kecacingan baik melalui kuku tangan ataupun kuku kaki.Tujuan Penelitian ini untuk mengidentifikasi telur cacing nematoda usus menggunakan metode sedimentasi pada sampel kuku petani sawah di wilayah Kelurahan Tanete Kecamatan Bulukumpa Kabupaten Bulukumba. Metode penelitian ini merupakan penelitian observasi laboratorik menggunakan metode sedimentasi dimana sampel yang digunakan sebanyak 21 sampel dengan teknik pengambilan secara purposive sampling. Berdasarkan Hasil penelitian sampel kuku petani yang telah diuji terdapat telur cacing nematoda usus yaitu 4% jenis Ascaris Lumbricoides dan 96% tidak terdapat telur cacing nematoda usus. Kesimpulan diharapkan agar masyarakat yang setiap hari bekerja dan kontak langsung dengan tanah, khususnya para petani sawah mampu memperhatikan kebersihan diri.

Gambaran Eritrosit Pada Sediaan Apusan Darah Tepi (ADT) Dengan Pewarnaan Giemsa Dan Ekstrak Kulit Manggis (Garcinia Mangostana) Anraeni Resbiani; Dzikra Arwie; Asriyani Ridwan
Jurnal TLM Blood Smear Vol 3 No 1 (2022): Jurnal TLM Blood Smear
Publisher : Stikes Panrita Husada Bulukumba

Pemanfaatan ekstrak kulit manggis yang dijadikan sebagai pewarnaan merupakan salah satu upaya pemanfaatan produk berbasis kulit buah manggis sebagai pewarna makanan alami Selain pewarnaan giemsa. Kulit buah manggis mengandung 59 ppm antosianin. Tujuan penelitian ini untuk melihat gambaran eritrosit pada sediaan ADT dengan menggunakan pewarnaan giemsa dan ekstrak kulit manggis (Garcinia mangostana) dengan menggunakan konsentrasi 20%, 40%, 60%, 80%, 100%. Jenis desain penelitian yang digunakan adalah experiment laboratory. Dalam penelitian ini menggunakan dua pewarnaan terhadap sediaan apusan darah tepi yaitu pewarnaan giemsa dan ekstrak kulit manggis (Garcinia mangostana) untuk melihat gambaran eritrosit. Hasil dari penelitian ini yaitu gambaran hasil pemeriksaan apusan darah tepi (ADT) berdasarkan tingkat konsentrasi, Konsentrasi 20% menunjukkan gambaran eritrosit lebih jelas dan sesuai dengan kualitas pewarnaan giemsa dibanding 40%, 60%, 80% dan 100%. Kesimpulan penelitian ini adalah pemeriksaan eritrosit pada apusan darah tepi (ADT) dengan menggunakan ekstrak kulit manggis dapat dilihat dengan konsentrasi 20%, 40%, 60%, 80%, 100% dan gambar eritrosit terlihat lebih jelas dan menyerupai atau sesuai dengan pewarnaan giemsa pada konsentrasi 20%.

Identifikasi Candida Albicans Pada Saliva Penderita Diabetes Melitus Di Wikayah Kerja Puskesmas Gattareng Tati Salmiati; Dzikra Arwie; Fatimah
Jurnal TLM Blood Smear Vol 2 No 1 (2021): Jurnal TLM Blood Smear
Publisher : Stikes Panrita Husada Bulukumba

Diabetes melitus atau yang biasa di singkat dengan DM adalah sekumpulan gejala yang timbul pada diri seseorang akibat tubuhnya mengalami gangguan dalam mengontrol kadar gula darah. Gula yang terdapat pada saliva menumpuk pada mukosa sehingga mempersiapkan makanan untuk perkembangan jamur, sehingga mengakibatkan saliva pada penderita akan ditemukan Candida albicans. Penelitian ini bertujuan untuk mengetahui adanya Candida albicans pada Saliva penderita Diabetes Melitus di wilayah kerja Puskesmas Gattareng Kecamatan Gantarang Kabupaten Bulukumba dengan menggunakan media SDA (Sabouraud Dextrose Agar). Penelitian ini merupakan penelitian observasi laboratory dengan jumlah sampel 16 yang diperiksa adanya jamur Candida albicans pada saliva penderita DM. Dari 16 sampel yang telah diperiksa didapatkan keseluruhan sampel positif tumbuh koloni pada media tanam SDA (Sabouroud Dextrose Agar) pada saliva penderita DM di wilayah kerja puskesmas gattareng kecamatan gantarang kabupaten bulukumba. Kata Kunci: Saliva, Diabetes Melitus, SDA, Candida albicans

Identifikasi Soil Transmitted Helminth (STH) Anak Usia 7-10 Tahun Menggunakan Sampel Feses Metode Natif Nurfadillah; Asriyani Ridwan; Dzikra Arwie
Jurnal TLM Blood Smear Vol 2 No 2 (2021): Jurnal TLM Blood Smear
Publisher : Stikes Panrita Husada Bulukumba

The background of this research is that Soil-Transmitted Helminth type worm infection is an infection that can be transmitted through the soil including Ascaris lumbricoides, Trichuris Trichur, and Hookworm. In Indonesia, worm infection is the most common health problem in urban and semi-urban areas that have poor sanitation, personal hygiene, and socioeconomic conditions. Intestinal nematode eggs are happy in areas where the environment is a slum, there is inorganic waste, and One of the locations in the Final Disposal Site (TPA). The aim of this research is to determine the type of intestinal nematode worm eggs or Soil-transmitted helminth (STH) in the feces of children aged 7-10 years in the area of the final disposal site in Borong Manempa Hamlet, Polewali Village Bulukumba 2020. This research method uses the native method with the Accidental Sampling technique. The conclusion of this study is that 2 cases of positive samples were found with a percentage of 10% infected with Soil-Transmitted Helminth from the Ascaris lumbricoid group of the 20 samples examined, and 18 samples were negative with Soil-Transmitted Helminth with a percentage of 90%.

The Salmonella sp. Antibody Detection: in Expectant with an Early Diagnosis of Hyperemesis Gravidarum Using Slide Widal Test Dzikra Arwie; Arfiani Nur; Subakir Salnus
Journal of Global Research in Public Health Vol. 5 No. 2 (2020): December
Publisher : Program Studi Ilmu Kesehatan Masyarakat IIK Strada Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/jgrph.v5i2.275

Typhoid fever is a global problem especially in countries with poor hygiene. The main etiologies in Indonesia are S. Typhi, S. enterica, Serovar enterica, and S. Paratyphi A. Pregnant women are very sensitive to infection from various microorganisms, physiologically the immune system in pregnant women decreases, possibly as a result of the tolerance of the mother's immune system to the baby, which is a semi-allogenic tissue. The aim of this research was to detect antibodies to Salmonella sp. in pregnant women with an early diagnosis of Hyperemesis gravidarum using the slide method widal test. Based on the results of research on salmonella sp antibody detection in pregnant women with an early diagnosis of hyperemesis gravidarum using the slide method widal test, there were 80% of salmonella sp. in pregnant women with an early diagnosis of hyperemesis gravidarum. 16 positive samples from 20 total samples, 20% of samples are positive for O series antigens, 40% of samples are positive for O and H series antigens, and 20% of samples are positive for H series antigens.

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