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All Journal Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention Majalah Obat Tradisional Acta Pharmaciae Indonesia : Acta Pharm Indo Molekul: Jurnal Ilmiah Kimia JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
. Harwoko
Department Of Pharmacy, Faculty Of Health Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Medicine & Pharmacology Public Health

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Journal : Molekul: Jurnal Ilmiah Kimia

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Phytochemical Analysis and Antioxidant Activity of Brotowali (Tinospora crispa L. Mier) Stem Warsinah, Warsinah; Baroroh, Hanif Nasiatul; Harwoko, Harwoko
Molekul Vol 15, No 2 (2020)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.873 KB) | DOI: 10.20884/1.jm.2020.15.2.533

Abstract

Free radical in the body could cause degenerative diseases such as diabetes mellitus and hyperlipidemia, which could be prevented by the supplementation of antioxidant whether it is synthetic or natural. Flavanoids is a phenolic compound, was extracted from natural sources is known for its antioxidative potential. This research aimed to investigate the active compounds in brotowali (Tinospora crispa) stem to find new antioxidative potential using its ability to bind 2, 2’-diphenyl-1-picrylhydrazyl (DPPH). Brotowali stem was extracted using ethanol and fractioned using n-hexane, ethyl acetate, and water. Water fraction was hydrolyzed by chloric acid. The antioxidant activity of ethanol extract, water fraction, and hydrolized water fraction (subfraction I and subfraction II) were determined using spectrophotometry (DPPH methods). The compound of flavonoid was identified using KLT with AlCl3 reagent spray. The results showed that ethanol extract and water fraction of brotowali stem contained flavonoid glycoside of flavonol group. Antioxidant activity, quantified using IC50, of ethanol extract, water fraction, subfraction I and subfractiont II were 49.92μg/mL, 38.25 μg/mL, 36.12 μg/mL, and 16.18 μg/mL, respectively. In addition, this research was found that hydrolysis of water fraction using chloric acid like in subfraction I and subfraction II was able to improve its antioxidant activity.
Co-Authors . Septiyaningsih A. Haris Budi Widodo Azhar, Ade Rizki Choironi, Nur Amalia Esti Dyah Utami Farah Amiria, Farah Fareza, Muhamad Salman Georgios Daletos Hanif Nasiatul Baroroh HENY EKOWATI Jeanita, Rani Saskia Jungho Lee Michael Feldbrügge Nuryanti Nuryanti Peter Proksch Rainer Kalscheuer Sunarto, Sunarto Warsinah Warsinah