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Muhaimin Rifa'i
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Aktivitas Ekstrak Etanol Umbi Uwi Ungu (Dioscorea alata L.) terhadap Sel B220+IgE+ pada Mencit BALB/c Model Alergi Pencernaan Yuyun Ika Christina; Muhaimin Rifa'i
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : University of Brawijaya

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Uwi ungu (Dioscorea alata L.) merupakan famili Dioscoreaceae yang mengandung diosgenin yang diketahui bermanfaat sebagai agen imunomodulator. Tujuan dari penelitian ini adalah untuk mengetahui adanya perubahan kuantitas sel B220+IgE+ pada organ lymph node mencit BALB/c model alergi pencernaan pasca pemberian ekstrak etanol umbi uwi ungu dan dosis optimum ekstrak etanol umbi uwi ungu. Pemberian ekstrak etanol umbi uwi ungu dilakukan pada hari ke 1-28. Hari ke-15 dilakukan injeksi ovalbumin (OVA) secara intraperitoneal. Kemudian 3 hari setelah injeksi OVA mencit dibedah. Hari ke-22 dilakukan challenge OVA secara intraperitoneal. Hari ke-23 sampai 28 mencit dilakukan injeksi OVA kembali secara oral. Kemudian dilakukan pembedahan pada hari ke-29. Limfosit diisolasi pada organ lymph node dan dilihat profil sel B220+ dan B220+IgE+ melalui analisis flow cytometry. Data dianalisis menggunakan One-way ANOVA (p<0,05) dan uji Tukey menggunakan SPSS 16  for Windows. Jumlah sel B220+IgE+ menurun signifikan pada dosis 0,167 g/kg BB dibandingkan pada dosis 2,008 g/kg BB dan 10,039 g/kg BB. Variasi dosis ekstrak etanol umbi uwi ungu berbeda secara signifikan. Ekstrak etanol umbi uwi ungu (Dioscorea alata L.) mampu menurunkan jumlah relatif sel B220+IgE+ pada dosis 0,167 g/kg BB. Dosis optimum untuk menurunkan level B220+IgE+ adalah pada dosis rendah yaitu 0,167 g/kg BB.Kata kunci : Alergi pencernaan, B220+IgE+, Dioscorea alata L., immunomodulator
Effectivity Combination of Bitter Melon (Momordica charantia) and Bitter (Andrographis paniculata) Extract to Suppress Proinflammatory Cytokines in Diabetic Mouse Models Firda Agustin; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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nsulin Resistance (IR) is main characteristic of Type 2 Diabetes Mellitus. IL-6 and IFNγ play important role in the deterioration of this disease. The aim of this experiment was to know effectivity combination of Bitter Melon and Bitter to decrease the expression of IL-6 and IFNγ in Type 2 Diabetes Mellitus. Type 2 Diabetes Mellitus were induced by injecting streptozotocin to neonate BALB/c mice in the age of five days (100 mg/kg BW). This experiment applied five groups which divided into  normal group, T2D (Type 2 Diabetes Mellitus model group), T2D-D1 (Bitter Melon dose 5,6 mg/kg BW and Bitter 20 g/kg BW), T2D-D2 (Bitter Melon doses 56 mg/kg BW and Bitter doses 200 mg/kg BW) and T2D-D3 (Bitter Melon doses 5600 mg/kg BW and Bitter doses 20000 mg/kg BW). Relative number of proinflammatory cytokines were analyzed using One-Way ANOVA with SPSS 16.0 for Windows. The results showed that the treatment with Bitter melon (Momordica charantia) and bitter (Andrographis paniculata) gave different effect compared to T2D groups. Medicinal herbs groups supressed proinflamatory cytokines such as IL-6 and IFNγ. So that relative number of IL-6 and IFNγ in treated group is lower than Type 2 Diabetes Mellitus (T2D) mice model.   Key words: IFNγ, IL-6, Insulin, Proinflammatory
Activity Test of Dexamethasone Therapy to Humoral Immunity in Balb/c Mice with Biliary Atresia Churi Wardah; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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The prevalence of biliary atresia is 1:5.000-8.000 of live births. Kasai portoenterostomy is a reliable treatment, but liver damage continues. Dexamethasone (corticosteroid) evolved into a commonly used therapy and is believed to improve clinical outcomes in biliary atresia. The objective of this study was to determine the effect of dexamethasone on the absolute number of IgA, IgD, IgE, IgG, and IgM in mice Balb/c model of biliary atresia. Murine model of biliary atresia obtained by injection of 20 μL of PBS containing 106 pfu Rhesus rotavirus (RRV) at <24 hours of mice age. Injection of dexamethasone with dose of 0.5 mg/kg body weight was done on day 7 to day 14, and day 14 to day 21 after virus injection. Spleen taken for analysis of flow cytometry. Data were tested with Kruskal-Wallis test, then follow up the Man-Whitney test to assess   the difference with SPSS 16.0 for Windows. Here, we show significant difference of immunoglobulin molecule in both termination (p<0.05). Subcutaneously injection of RRV (Rhesus Rotavirus) in Balb/c mice in the of aged <24 hours is capable to stimulate the production of immunoglobulin, especially at the termination of the third week. This can be evidenced by an increase in IgD, IgA, IgD, and IgG. Dexamethasone is glucocorticoid that plays role as immunosuppressant with ability in decreasing IgD, IgM, IgD, and IgG levels in both termination. Furthermore we showed that dexamethasone was capable of stimulating the production of IgA in third week. Keywords : Balb/c baby mice, Biliary atresia, Corticosteroid, Dexamethasone, Rhesus Rotavirus
Activity Test of VipAlbumin® against Blood Glucose Levels and Lymfhocytes Profiles in Balbc mice as Type 2 Diabetes Mellitus model Ganys Tri Silvana; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : University of Brawijaya

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Activity Test of VipAlbumin® against Blood Glucose Levels and Lymfhocytes Profiles in Balb/c mice as Type 2 Diabetes Mellitus model   Ganys Tri Silvana1), Muhaimin Rifa’i2) 1) Biology Department, Faculty of Science, Brawijaya University, Malang, Indonesia   Abstract Diabetes mellitus (DM) has a prevalence that is increase from year to year, so we need an effective and economical drugs to cope. VipAlbumin® have high antioxidant content, so it can be used as an effective diabetes therapy. The purpose of this study was to determine the effect VipAlbumin® derived from Fish Cork (Channa striata) in decreasing blood glucose levels and assessing relative number of T cells. Mice models of  diabetes mellitus (DM) were created by injecting treptozotocin in  mice at the age of 5 days (streptozotocin in 1 ml of citrate buffer solution, a dose of 100 mg / kg BW). Mice were divided into 5 groups (Normal mice, DM, DM-D1, DM-D2 and DM-D3). After 3 weeks, the levels of blood glucose was examined and then oral therapy with VipAlbumin® was performed for 15 days. Profiles of CD4+, CD8+, and CD4+CD62L+ T cells were analyzed by flow cytometry by isolating splenic cells. One-way ANOVA was used to analyze the data. The differences between groups were considered significant at P<0.05. All results were presented as the mean of ± SD values of 5 mice in each group. In this experiment we showed that VipAlbumin® have a capability to prevent T cells activation by increasing naïve type status of both CD4 and CD8 population in mice model of DM. The number of CD4+ and CD8+ T cells decreased significantly in a dose of D3. Effective dose to decrease blood sugar in mice with type 2 diabetes is on the D3.   Keyword : Diabetes Melitus, Flowcytometry, Lympocytes, Streptozotocin, VipAlbumin®.
Expression of Erythroid Progenitor Cells and Erythrocytes on Dexamethasone Induced-Mice Wira Eka Putra; Muhaimin Rifa&#039;i; Aris Soewondo
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : University of Brawijaya

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Dexamethasone (Dex) is synthetic corticosteroid, known as anti-inflamation drug to ameliorate autoimmune diseases. It worked by inhibiting production of proinflamatory citokines. The aim of this experiment was to confirm the effect of administration of Dex on erythroid progenitor cells, TER-119+VLA-4+ and erythrocytes, TER-119+VLA-4- expression from bone marrow compartments. Two weeks old BALB/c mice were used and grouped into 3 injection treatments of Dex with six replications i.e. no injection (control), 0.5 mg/kg BW (dose 1), 10 mg/kg BW (dose 2). To investigate the therapeutic effect of dexamethasone, the mice were sacrified on day-7. The bone marrow cells were isolated and analyzed by flow cytometry. Data analysis was confirmed with the Kruskal-Wallis test followed by Mann-Whitney test with significance level (α) of 0.05. The result showed that administration of Dex on BALB/c mice increase the expression of erythroid progenitor cells, TER-119+ VLA-4+ and erythrocytes, TER-119+VLA-4- from bone marrow.Key words: Dex, erythrocytes, erythroid progenitor cells
Uji Aktifitas Biologis Fraksi Ethanol Daun Sambiloto (Andrographis paniculata) Terhadap Perubahan Kuantitatif Sel T Regulator Pada Mencit BALB/c (Mus musculus) Uwais Al Qarni; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 1, No 5 (2013)
Publisher : University of Brawijaya

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Andrografolid merupakan senyawa aktif sejenis diterpenoid yang terkandung dalam daun sambiloto dan berperan sebagai imunomodulator di dalam sistem imun. Penelitian ini bertujuan untuk mengetahui perubahan kuantitatif sel T regulator setelah pemberian fraksi ethanol daun sambiloto secara oral, mengetahui dosis optimum fraksi ethanol daun sambiloto dalam perubahan kuantitas sel T regulator, dan mengetahui histopatologis jaringan hepar setelah pemberian fraksi ethanol daun sambiloto. Metode meliputi ekstraksi daun sambiloto dengan pelarut ethanol 95%, perlakuan oral pada mencit BALB/c normal selama 2 minggu dengan dosis 0; 0,1; 0,5; dan 1 mg/g BB, isolasi sel limfosit organ spleen, analisis kuantitatif sel limfosit menggunakan haemocytometer dan flowcytometry, dan pembuatan preparat histologi hepar dengan pewarnaan HE. Analisis data menggunakan Rancangan Acak Lengkap (RAL) dengan uji ANOVA selang kepercayaan 95% menggunakan program SPSS 16 for Windows. Pemberian fraksi ethanol daun sambiloto secara oral meningkatkan jumlah sel T CD4+CD25+, pada Dosis 1 (0,1 mg/g BB) dan Dosis 2 (0,5 mg/g BB), selain itu terjadi penurunan jumlah sel T CD4+, CD4+CD62L+, dan CD8+CD62L+ pada Dosis 1 (0,1 mg/g BB) dan Dosis 3 (1 mg/g BB). Dosis optimum fraksi ethanol daun sambiloto dalam peningkatan sel T regulator adalah 0,1 mg/g BB. Fraksi ethanol daun sambiloto dosis 1 mg/g BB menimbulkan efek toksik yang signifikan pada jaringan hepar.   Kata kunci: andrografolid, daun sambiloto, fraksi ethanol, histopatologi, imunomodulator
Studi Ekstrak Metanol Biji Mahoni (Swietenia mahagoni) terhadap SOD Serum Tikus Putih (Rattus novergicus) Pasca Induksi MLD-STZ Deasy Luandayanti; Muhaimin Rifa&#039;i; Aulanni&#039;am Aulanni&#039;am
Biotropika: Journal of Tropical Biology Vol 1, No 6 (2013)
Publisher : University of Brawijaya

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Diabetes mellitus (DM) merupakan suatu gejala yang timbul pada seseorang dimana keadaan hiperglikemia (kadar gula yang tinggi) yang kronik dengan disertai berbagai kelainan metabolik akibat gangguan hormonal. Salah satu tumbuhan yang digunakan masyarakat untuk pengobatan DM adalah mahoni (Swietenia mahagoni). Bagian yang digunakan dari tumbuhan tersebut adalah bijinya. Namun mekanisme ekstrak metanol biji mahoni belum banyak diketahui. Pada penelitian ini menggunakan tikus model DM dengan induksi MLD-STZ. Tikus dikelompokkan dalam 5 kelompok, yaitu kelompok kontrol negatif, kontrol positif, dan  kelompok DM dengan terapi 100, 250 dan 400 mg/KgBB. Metode yang digunakan dalam penelitian ini meliputi pengekstrakan biji mahoni, induksi DM dengan injeksi MLD-STZ secara intraperitonial dan uji aktivitas SOD serum. Hasil penelitian menunjukkan bahwa  nilai SOD serum yang didapatkan berdasarkan kelompok, yaitu 98,99; 61,12; 70,05; 79,29 dan 84,49 unit/ml. Pemberian terapi ekstrak metanol biji mahoni pada tikus model DM mampu menaikkan aktivitas SOD. Kata Kunci : diabetes mellitus (DM), SOD (superoksida dismutase), STZ (streptozotocin)
Analisa Flow Cytometry pada Subpopulasi Sel T-Limfosit Bursa Fabricius Ayam Pedaging Pasca Infeksi Salmonella typhimurium dan Pemberian Pakan Tambahan Polyscias obtusa septi utami dewi; Mochammad Sasmito Djati; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 1, No 5 (2013)
Publisher : University of Brawijaya

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Tujuan penelitian  ini adalah mengetahui adanya aktivitas imunomodulator daun Polyscias obtusa pada organ bursa fabricius ayam  pedaging  yang terinfeksi bakteri Salmonella typhimurium, serta mengetahui dosis optimum penambahan daun Polyscias obtusa dalam mempengaruhi jumlah subpopulasi T-limfosit pada organ bursa fabricius ayam pedaging yang terinfeksi Salmonella typhimurium. Prosedur kerja yang dilakukan adalah konfirmasi isolat bakteri Salmonella typhimurium, pemberian daun Polyscias obtusa, infeksi bakteri Salmonella typhimurium, pembedahan, isolasi sel, analisis Flow Cytometri, analisis data. Hasil penelitian menunjukkan bahwa Pemberian pakan alami dengan tambahan daun Polyscias obtusa dengan variasi dosis 0,08%, 0,16%, tidak memberikan pengaruh terhadap jumlah relatif CD8+ dan B220+ pada Bursa fabricius. Sehingga, tidak ada perbedaan yang signifikan antara pakan pabrik dan pakan alami dengan tambahan daun Polyscias obtusa.. Namun, pada dosis 0,26 % fase finisher terlihat peningkatan jumlah relatif sel CD4+ yang siginifikan dibandingkan fase starter. Dapat disimpulkan bahwa, dosis 0,26% merupakan dosis optimum dalam meningkatkan proses proliferasi jumlah sel CD4+ sebagai agen imunomodulator pada bursa fabricius ayam pedaging.   Kata kunci :  Ayam pedaging, bursa fabricius, flow cytometry,   Polyscias obtusa, Salmonella typhimurium typhymurium
Dexamethasone Activities toward Population of B cells, Gr-1, and TNF-α cytokine in Mice (Musmusculus) Balb/c Biliary Atresia Model Riza Rahmawati; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : University of Brawijaya

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Biliary atresia iscondition caused by Rotavirus (RRV) infection. The aims of this study were to know the immune responses of mice model of biliary atresia treated with corticosteroid.Mice were splitinto 3 treatment groups: control (K), RRV injection (R), and RRV injection in the present of dexamethasone(R+D). In R treatment, the baby mice born in <24 hours were injected with 20 µl of phosphate buffered saline containing 1.5 x 106 fluorescence-forming units Rhesus Rotavirus (RRV). First termination was performed in the day 7 to 14, while second termination was done in the day 14 to 21. The dosage of dexamethasone which is applied in this experiment is 0.5mg/kg body weight.Immunocompetent cells were isolated from spleen,  and cell surface molecules were then analyzed by flowcytometry. The data was tested by SPSS 16.0 for Windows program. The results showed that dexamethasone given as corticosteroid for biliarry atresia theurapy couldsuppress TNF-αproduction as well as Gr-1 proliferation. In the other hand dexamethasonecan promote B220+ cell proliferation inrotavirus infected mice. Key word: Baby mice, biliary atresia, dexamethason, flowcytometry, rotavirus
The Effect of Dexamethasone Treatment to Humoral Immunity in BALB/C Mice Models Ovi Ardiana; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 3, No 3 (2015)
Publisher : University of Brawijaya

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ABSTRACT Glucocorticoids (GCs) are a class of steroid hormones which regulate a variety of essential biological functions. The profound anti-inflammatory and immunosuppressive activity of synthetic GCs, combined with their power to induce lymphocyte apoptosis place them among the most commonly prescribed drugs worldwide. Endogenous GCs also exert a wide range of immunomodulatory activities, including the control of T cell homeostasis. Dexamethasone is kind of glucocorticoid which is used to control some deseases. The purpose of this experiment is to know the effect of dexamethasone on B220. There are three different doses of treatments applied: 0.0 mg/kg BW (control), 0.5 mg/kg BW, and 10 mg/kg BW.  Each treatment uses 6 mice (2 weeks old) with intraperitoneal injection. The treated mice were observed for 7 days and spleen cells were isolated for flow cytometric immunophenotyping . The data were analyzed by BD CellQuest and tested using One-way ANOVA (p<0,05) then Tukey’s test with SPSS 16.0 for Windows program. The result of this experiment showed that the dose of 0.5 mg/kg BW did not decrease B220 cell number significantly, but the dose of 10 mg/kg BW did. Keyword : B220, Dexamethasone, Glucocorticoids
Co-Authors Aris Soewondo Aulanni'am, Aulanni'am Churi Wardah Deasy Luandayanti Emi Rohmawati Firda Agustin Ganys Tri Silvana Iga Dwi Januarisasi Lailiya Vina Rochmatika Moch Sasmito Djati Ovi Ardiana Qonitatul Khasanah Riza Rahmawati septi utami dewi Uwais Al Qarni Wira Eka Putra Yonna Ayundria Yuyun Ika Christina