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All Journal Biogenesis: Jurnal Ilmiah Biologi Biotropika The Journal of Pure and Applied Chemistry Research Biota Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Wira Eka Putra
Universitas Negeri Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Expression of Erythroid Progenitor Cells and Erythrocytes on Dexamethasone Induced-Mice Putra, Wira Eka; Soewondo, Aris
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.018 KB)

Abstract

Dexamethasone (Dex) is synthetic corticosteroid, known as anti-inflamation drug to ameliorate autoimmune diseases. It worked by inhibiting production of proinflamatory citokines. The aim of this experiment was to confirm the effect of administration of Dex on erythroid progenitor cells, TER-119+VLA-4+ and erythrocytes, TER-119+VLA-4- expression from bone marrow compartments. Two weeks old BALB/c mice were used and grouped into 3 injection treatments of Dex with six replications i.e. no injection (control), 0.5 mg/kg BW (dose 1), 10 mg/kg BW (dose 2). To investigate the therapeutic effect of dexamethasone, the mice were sacrified on day-7. The bone marrow cells were isolated and analyzed by flow cytometry. Data analysis was confirmed with the Kruskal-Wallis test followed by Mann-Whitney test with significance level (α) of 0.05. The result showed that administration of Dex on BALB/c mice increase the expression of erythroid progenitor cells, TER-119+ VLA-4+ and erythrocytes, TER-119+VLA-4- from bone marrow.Key words: Dex, erythrocytes, erythroid progenitor cells
Expression of Erythroid Progenitor Cells and Erythrocytes on Dexamethasone Induced-Mice Wira Eka Putra; Muhaimin Rifa'i; Aris Soewondo
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : University of Brawijaya

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Abstract

Dexamethasone (Dex) is synthetic corticosteroid, known as anti-inflamation drug to ameliorate autoimmune diseases. It worked by inhibiting production of proinflamatory citokines. The aim of this experiment was to confirm the effect of administration of Dex on erythroid progenitor cells, TER-119+VLA-4+ and erythrocytes, TER-119+VLA-4- expression from bone marrow compartments. Two weeks old BALB/c mice were used and grouped into 3 injection treatments of Dex with six replications i.e. no injection (control), 0.5 mg/kg BW (dose 1), 10 mg/kg BW (dose 2). To investigate the therapeutic effect of dexamethasone, the mice were sacrified on day-7. The bone marrow cells were isolated and analyzed by flow cytometry. Data analysis was confirmed with the Kruskal-Wallis test followed by Mann-Whitney test with significance level (α) of 0.05. The result showed that administration of Dex on BALB/c mice increase the expression of erythroid progenitor cells, TER-119+ VLA-4+ and erythrocytes, TER-119+VLA-4- from bone marrow.Key words: Dex, erythrocytes, erythroid progenitor cells
Effect of Dexamethasone Administration toward Hematopoietic Stem Cells and Blood Progenitor Cells Expression on BALB/c Mice Wira Eka Putra; Aris Soewondo; Muhaimin Rifa'i
The Journal of Pure and Applied Chemistry Research Vol 4, No 3 (2015)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (815.457 KB) | DOI: 10.21776/ub.jpacr.2015.004.03.221

Abstract

Hematopoiesis play important roles on maintaining body homeostasis. Dexamethasone (Dex) known as synthetic glucocorticoids and widely affect many regulations i.e. immune system, inflammation, reproduction and all aspects of metabolism. Generally Dex were used as therapeutic agentsto ameliorate many kind of diseases including cancer and auto-immune diseases. The aims of this research were to investigate the effect of Dex administration toward expression of hematopoietic stem cells and blood progenitor cells. Two weeks old BALB/c mice were used and treated with 3 groups of Dex administration i.e. untreated-mice, 0.5 mg/kg BW, and 10 mg/kg BW. Mice were sacrificed on day-7 after administration of Dex. The bone marrow stromal cells were isolated, then followed by flow cytometry analysis. The result showed that, Dex administration with dose 0.5 mg/kg BW significantly increase the expression of hematopoietic stem cells, CD34+, chemokines, SDF-1+, erythroid progenitor cells, Gr-1-TER-119+, granulocytes, Gr-1+, and B progenitor cells, B220+.
Identification of Edible Macrofungi at Kerandangan Protected Forest & Natural Park, West Lombok Regency, Indonesia Ahmad Hapiz; Sukiman Sukiman; Faturrahman Faturrahman; Bagus Priambodo; Facthur Rohman; Hendra Susanto; Wira Eka Putra
Biota Vol 13 No 1 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i1.250

Abstract

Indonesia is considered as a mega-biodiversity country that has a massive amount of vascular and non-vascular plants. The tropical environment condition of Indonesia could support the growth of macrofungi. Information about edible macrofungi from the Forest of Lombok Island is based on limited data. This research aims to characterize the edible macrofungi at Kerandangan Protected Forest & Natural Park, West Lombok Regency, Indonesia. This research was a descriptive and explorative study. The edible mushrooms were observed through the Cruise method by following the particular track inside the forest. The sample found in the forest then documented and evaluated. A morphological analysis procedure was performed to assess the profile and similarity between the microscopic evaluations with the mushrooms identification book. In this study, we also offered a phylogeny analysis based on morphological characters similarity. The Dendogram tree was reconstructed using PAST 3.0. software. The result showed that there are eight species of edible mushrooms found that were group into Basidiomycota, namely, Termitomyces clypeatus, Termitomyces umkowaan, Termitomyces sp.1, Pleorotus flabelatus, Pleurotus ostreatus, Coprinus desimenatus, Tremella fuciformis, and Polyporus sp. All of them involved in Basidiomycota. Based on characterization and identification, the species are grouped into three Ordos and five Families. Most of the edible macrofungi found at Kerandangan Protected Forest & Natural Park growth at soil and deadwood.
Anti-liver fibrosis of green materials Moringa oleifera seed oils from Madura Island against hepatocellular carcinoma development Hendra Susanto; Ahmad Taufiq; Nik Ahmad Nizam Nik Malek; Wira Eka Putra
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 1 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i1.27464

Abstract

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Target protein prediction of Indonesian jamu kunyit asam (Curcumin-tamarind) for dysmenorrhea pain reliever: A network analysis approach Galuh Wening Permatasari; Mochammad Fitri Atho'illah; Wira Eka Putra
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 12, No 3, (2021)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol12.Iss3.art7

Abstract

Background: Dysmenorrhea is a monthly problem for reproductive-age women before or during menstruation. Dynamic changes of prostaglandins (PGs) and progesterone in the late reproduction cycle regulating signal pathways of PGE2 and PGF2 can contribute to menstrual cramps and other dysmenorrhea symptoms. Curcuma longa and Tamarindus indica (CT) are traditional medicines more preferred by Indonesian women to overcome menstrual pain than painkiller drugs.Objective: This study aims to observe potentially active compounds and detailed mechanism of Curcuma longa and Tamarindus indica (CT) as a pain reliever.Methods: Bioactive compounds in the CT were collected from two databases and then screened by bioavailability and drug-likeness parameter by utilizing SwissADME software. The target proteins and genes associated with dysmenorrhea were retrieved from HitPick and GeneCard database. The possibility of how CT healed dysmenorrhea was predicted by protein-protein interaction (PPI) using STRING Webserver and was visualized by Cytoscape v.3.7.0 and REVIGO.Results: Totally, 147 active compounds were obtained from C. longa and 212 active compounds from T. indica. Respectively, 36 and 66 compounds were obtained from C. longa and T. indica by the ADME screening. Finally, 20 and 22 target proteins were identified in both herbs. Then, an overlap analysis of this study showed that four target proteins had strong interaction with pain-related dysmenorrheaConclusion: This study found possible target proteins of the CT to relieve dysmenorrhea. The target proteins included HSD17B1, ALOX5, GSTM1, and ESR2 genes. Further, these findings are needed to be validated by using in vitro and in vivo approaches.
Penyegaran Keterampilan Dasar Bekerja di Laboratorium Mikrobiologi untuk Persiapan Tugas Akhir dan Menghadapi Dunia Kerja Wira Eka Putra; Mardiana Lelitawati; Intan Chairun Nisa; Evi Susanti; Salsabila Kasta Hygiea Iswara; Syafira Maharani
ABDI: Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol 4 No 2 (2022): Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat
Publisher : Labor Jurusan Sosiologi, Fakultas Ilmu Sosial, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/abdi.v4i2.310

Abstract

Basic laboratory skills are essential for supporting the quality of learning outcomes and processes, and they are also very beneficial as preparation for the workplace. The goal of this activity is for students and graduates of the Faculty of Mathematics and Natural Sciences (FMIPA) to refresh and develop their basic abilities in working in a microbiological laboratory in preparation for their final assignment and the workplace. During COVID-19 pandemic, the agenda is carried out in two methods, namely online and offline systems. The training was conducted online using a video conferencing platform and an online academic information system, as well as offline at the microbiology laboratory, Department of Biology, FMIPA, Universitas Negeri Malang. The participants' comprehension of basic microbiology concepts and basic abilities for working in a microbiology laboratory improved as a result of this exercise according to the pretest and posttest result.
Co-Authors Ahmad Hapiz Ahmad Taufiq Aris Soewondo Bagus Priambodo Evi Susanti Facthur Rohman Faturrahman Galuh Wening Permatasari Hendra Susanto Intan Chairun Nisa Mardiana Lelitawati Mochammad Fitri Atho'illah Muhaimin Rifa'i Muhaimin Rifa'i Nik Ahmad Nizam Nik Malek Salsabila Kasta Hygiea Iswara Sukiman Sukiman Syafira Maharani