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All Journal Biotropika Journal of Degraded and Mining Lands Management Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian
Luqman Qurata Aini
Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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EKSPLORASI KAPANG ANTAGONIS DAN KAPANG PATOGEN TANAMAN APEL DI LAHAN PERKEBUNAN APEL PONCOKUSUMO Galuh Setyanto Pradana; Tri Ardiyati; Luqman Qurata Aini
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : University of Brawijaya

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Abstract

Poncokusumo merupakan daerah pertanian di Kabupaten Malang yang memiliki komoditas utama yaitu apel. Serangan kapang patogen menyebabkan apel menjadi rusak dan busuk. Selama ini, pengendalian kapang patogen hanya dilakukan dengan menggunakan pestisida dan mampu menyebabkan terjadinya resistensi kapang patogen. Penelitian ini bertujuan mengetahui spesies kapang antagonis dan kapang patogen yang terdeteksi serta mengetahui tingkat dan mekanisme penghambatan kapang antagonis terhadap pertumbuhan kapang patogen dari lahan perkebunan apel Poncokusumo. Isolasi kapang antagonis dilakukan menggunakan sampel tanah top soil 10 cm, sedangkan isolasi kapang patogen berasal dari sampel organ tanaman yang terserang penyakit dengan melakukan sterilisasi menggunakan larutan NaOCl 5 %. Isolat kapang antagonis diuji tingkat penghambatannya terhadap kapang patogen tanaman apel dengan menggunakan metode dual culture dan slide culture. Persentase penghambatan masing-masing kapang antagonis dianalisis secara statistik menggunakan one-way ANOVA. Hasil eksplorasi didapatkan tiga genus kapang patogen yaitu Venturia sp., Colletotrichum sp., dan Monilia sp., sedangkan kapang antagonis yang didapat antara lain Trichoderma sp.(I), Trichoderma sp.(II), Trichoderma sp.(III), Aspergillus sp.(I), dan Aspergillus sp.(II). Penghambatan terbaik ditunjukkan kapang antagonis Trichoderma sp.(I) yaitu menghambat Venturia sp. sebesar 50,51%, Colletotrichum sp. sebesar 73,30%, dan Monilia sp. sebesar 66,97%. Hasil pengamatan mikroskopis diketahui bahwa mekanisme penghambatan kapang antagonis terhadap kapang patogen menggunakan metode slide culture diketahui bahwa isolat Genus Trichoderma yaitu kompetisi dan parasitisme, sedangkan isolat Genus Aspergillus dengan antibiosis. Kata kunci: antagonis, eksplorasi, patogen, penghambatan.
Application of bacterial isolates to mitigate the effects of salt stress on red chilli growth and yields Nurul Aini; Wiwin Sumiya Dwi Yamika; Luqman Qurata Aini; Puput Wahyuningsih
Journal of Degraded and Mining Lands Management Vol 6, No 3 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2019.063.1771

Abstract

The objective of this research was to study the effect of bacterial isolates on red pepper at the saline condition. The research conducted in the station of Brawijaya University in Jatikerto village, Malang regency. The study used a randomized block design with bacteria isolated from the soil of saline-prone regions of Lamongan, in coastal East Java Indonesia. The treatments consisted of P0: saline soil without bacteria, P1: saline soil + bacteria SN 13, P2: saline + bacteria SN 22, P3: saline soil + bacteria SN 23, P4: saline soil + bacteria (SN 13 + SN 22), P5: saline soil + bacteria (SN 13 + SN 23), P6: saline soil + bacteria (SN 22 + SN 23), and P7: saline soil + bacterial (SN 13 + SN 22 + SN 23). Red chilli variety used was Gada MK F1. The results showed that the application of bacterial isolates increased leaf area, dry weight of roots and shoot. Uptake of N, P, K and Na also increased with bacterial isolate application. The number of fruit and fruit weight increased by 11.94% and 15.93%, respectively, compared with no bacteria. Proline content increased with the application of bacterial, while the capsaicin content decreased. 
KARAKTERISASI BAKTERI PATOGEN PENYEBAB LAYU PADA TANAMAN BAWANG MERAH (Allium ascalonicum L.) Intan Fuji Arriani; Abdul Latief Abadi; Luqman Qurata Aini
Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Vol 14 No 1 (2020): Mei 2020
Publisher : Universitas Islam Balitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35457/viabel.v14i1.1004

Abstract

Shallot (Allium ascalonicum L.) is one of the vegetable commodities in the form of tubers that have a high economic value. The development of shallot cultivation in Indonesia often experiences obstacles, one of which is an obstacle in the process of shallot cultivation, namely the attack of Plant Disturbing Organisms (OPT). Information about diseases caused by pathogenic bacteria is still very limited. This study aims to determine the symptoms and identification of pathogenic bacteria that cause wilt in shallots. Bacterial isolation was carried out using Nutrient Agar (NA) media and 36 bacterial isolates were collected from shallots. Bacterial isolates were then tested for pathogenicity to determine the ability of bacteria to cause wilt disease in shallots. The results of isolation obtained 10 bacterial isolates that can show symptoms on red onions namely wilted leaves, yellow and soft rotten tubers. Four isolates including positive can show hypersensitivity symptoms, namely M11, N20, N17 and N14. Based on the identification of bacteria in physiology showed 2 groups of different isolates. Biochemical test results of Isolate M11, N20 and show species suspected of B. cepacia. N3 and N14 isolates are suspected to be E. carotavora subsp. carotavora. The bacterial isolates N7, N17, P5 and P7 were suspected to be E. carotavora subsp. betavasculorum. The isolate of N4 bacteria is suspected to be E. cacticida.
Co-Authors Abdul Latief Abadi Galuh Setyanto Pradana Intan Fuji Arriani Nurul Aini Puput Wahyuningsih Tri Ardiyati Wiwin Sumiya Dwi Yamika