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Karakterisasi Produk Biosolubilisasi Lignit oleh Kapang Indigenus dari Tanah Pertambangan Batubara di Sumatera Selatan Sugoro, Irawan; Hermanto, Sandra; Sasongko, Dwiwahju; Indriani, Dea; Aditiawati, Pingkan
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTCharacterization of Lignite Biosolubilization Products by Indigenous Moulds from Soil ofCoal Mining in South Sumatera. Biosolubilization of coal is a potential technology of convertingsolid coal to liquid fuel and chemicals at ambient condition. Our previous research hassuccessfully isolated four moulds from soil at coal mining - South Sumatera and has potency aslignite biosolubilization agent, i.e. T1, T2, T4, T5. The objective of this research was to characterizeof lignite biosolubilization products by four isolates. The method used was sub-mergedculture. Cultivation medium was MSS+ (minimal salt + sucrose 0,1% + yeast extract 0,01% +lignite 5 %). Incubation was conducted at room temperature for 28 days. The result showed thatall indigenos moulds have different ability in lignite biosolubilization. The highestbiosolubilization occurred after 7 days of incubation belonging to T1 isolate. However, GC-MSanalysis showed the largest percentage of hydrocarbon compound which equivalent to gasolineand diesel was T5 after 7 days of incubation.Key words: Biosolubilization, lignite, moulds, coal.

ACE Inhibitory and Antioxidative Bioactive Peptides Derived from Hydrolyzed Soy Milk Hermanto, Sandra; Septiana, Annisa; Putera, Deni K; Hatiningsih, Fitriah; Muawanah, Anna
Molekul Vol 14, No 1 (2019)
Publisher : Universitas Jenderal Soedirman

Soy milk is a soybean processed product rich in protein as well as sources of bioactive peptides. Bioactive peptides defined as specific protein fragments that have a positive impact on body functions and conditions and may ultimately influence health. This study was conducted to explore the potential of hydrolyzed soy milk as a source of antioxidative and antihypertensive bioactive peptides through enzymatic hydrolysis. The initial treatment of soy milk protein was acidic precipitation with hydrochloric acid. Furthermore, protein precipitate was hydrolyzed using pepsin proteolytic enzyme with an enzyme: substrate ratio (1:5, 1:10 and 1:20). Protein hydrolysis was carried out for 0?48 hours at 37 °C in an acetate buffer pH 4.5. The soy milk protein hydrolysates were subjected to determination of % DH (Degree of Hydrolysis) and protein profile by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). The antihypertensive assay was carried out by in vitro inhibition of the ACE enzyme (Angiotensin Converting Enzyme) and antioxidative activity assay using the DPPH method. The results showed that the optimum conditions for hydrolysis of soy milk were obtained at 40 hours with a % DH value of 53.24% in enzyme ratio 1:20 and the highest antihypertensive activity was obtained from 48 hours hydrolysis with % ACE inhibition value of 79.31%. The highest antioxidative activity of bioactive peptide was obtained at hydrolysis 48 hours with IC50 69.1034 µg / ml. After fractionated and LCMS characterized it was obtained 2 bioactive peptides with molecular weights of 8.954 and 2,696 kDa. These bioactive peptides from hydrolyzed of soymilk might be potential as an antihypertensive agent and reduce oxidative stress.

Virtual Screening Peptida Bioaktif Antihipertensi dari Hidrolisat Kasein Susu Kambing Etawa Hermanto, Sandra
ALCHEMY Vol 5, No 2 (2016): ALCHEMY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Penapisan peptida bioaktif dari hidrolisat kasein susu kambing Etawa yang berpotensi sebagai obat antihipertensi berdasarkan kajian in silico telah dilakukan. Protein yang digunakan adalah Î±-S1-kasein prekursor [Capra hircus] NCBI Reference Sequence: NP_001272624.1, Î±-S2-kasein prekursor [C. hircus] NCBI Reference Sequence: NP_001272514.1, Î²-kasein [C. hircus] NCBI Reference Sequence: AAA30906.1 dan Îº-kasein prekursor [C. hircus] NCBI Reference Sequence: NP_001272516.1. Perancangan struktur peptida bioaktif dilakukan melalui simulasi hidrolisis enzimatik dengan menggunakan 3 jenis enzim proteolitik (tripsin, kimotripsin dan pepsin) dan dilanjutkan dengan preparasi struktur 3D ligan hasil pemotongan secara in silico. Virtual screening terhadap fragmen peptida dilakukan melalui penentuan nilai drug likeness dan protease inhibitor. Dari 104 fragmen peptida diperoleh 10 kandidat peptida bioaktif yang dilakukan simulasi molecular docking dengan mengeksplorasi daya inhibisi fragmen melalui perhitungan nilai (âˆ†Gbinding) dan interaksi antara kandidat peptida bioaktif dengan residu asam amino pada sisi aktif enzim ACE (Angiotensin Converting Enzyme). Sebagai kontrol positif digunakan lisinopril yang merupakan inhibitor ACE komersil. Hasil penelitian menunjukkan dari 10 kandidat peptida bioaktif terdapat 6 peptida yang diduga bersifat antihipertensi dengan nilai âˆ†GbindingÂ yang lebih rendah dari kontrol positif (lisinopril). Keenam peptida tersebut diharapkan dapat berfungsi sebagai obat alternatif antihipertensi.

Differentiation of Bovine and Porcine Gelatin Based on Spectroscopic and Electrophoretic Analysis Hermanto, Sandra; Sumarlin, La Ode; Fatimah, Widya
Journal of Food and Pharmaceutical Sciences Vol 1, No 3 (2013): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

This study was conducted to explore the differentiation of bovine and porcine gelatins before and after pepsin hydrolysis based on peptide pattern from spectroscopic and electrophoretic analysis due to development of the halal food products analysis. In this study, pepsin (EC 3.4.23.1)Â was used to hydrolyze the two sources of gelatin with consideration to its ability to digest up to 20% of ingested amide bonds by cleaving preferentially after the N-terminal of aromatic amino acids such as phenylalanine, tryptophan, and tyrosine. In this study, we expect to produce the fragment of gelatins with differentiation in relative molecular weights. Gelatins fragments then analyzed by UV-Vis and FTIR spectroscopy to characterize the functional groups on each source of gelatins, followed by SDS-PAGE (Sodium Duodecylsulphate Polyacrylamide Gel Electrophoresis) to identify the molecular weight of the resulting fragments. In UV-Vis spectroscopy, both gelatin source before and after hydrolysis had different absorbance at 229 nm and 240 nm showing the proportion of C=O amida and differences in two-dimensional conformation of the peptide. In terms of FTIR spectra, both gelatin have wavenumber at 3300-3400 cm-1 (NH stretching), 1600 cm-1 (C=O stretching, amida), 1500 cm-1 (C-N stretching), and at 620-767 cm-1 (OCN bending). This indicates that the relative amino acid compositions from two sources of gelatins were relatively different. In contrast, SDS-PAGE analysis does not give a real differentiation, except for porcine gelatin, that fragments which on 2 hour incubation show two peptide fragments with molecular sizes below 36,2 kDa and 28.6 kDa.

ELECTRICAL STUNNING AND PRE-SLAUGHTERING TIME INTERVAL EFFECT IN EXPRESSION OF HEART BROILER CHICKENS PROTEIN Hermanto, Sandra
Journal of Food and Pharmaceutical Sciences Vol 3, No 3 (2015): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Pre-slaughtering process by transporting broiler chickens from farm to slaughterhouse and treatment of electrical stunning could be one of halal critical point. Stressing on chickens could be trigger a specific protein expression or increase its expression as a result of changes in metabolism that occurs. The purpose of this study was to identify the effect of treatment of electrical stunning and the pre-slaughtering interval time on broiler chicken liver protein expression through the analysis of protein profile by SDS-PAGE. Experiments conducted on 7 broiler chickens aged 35 days were given pre-slaughter treatment are different. Treatments include electrical stunning of 0 V, 25 V and 250 V as well as the pre-slaughtering time interval 0 hours, 2 hours, 2 hours + 12 hours (fasting) and 2 hours + 12 (fasting) with electrical stunning 250 V. Each of samples (chicken liver protein) isolated by homogenized in Trs-HCl buffer and the protein concentration determined by Lowry method. Identification of the protein profile was done by observing the differences in the intensity of the electrophoresis results followed by densitometry analysis. The results showed significant differences in outcomes electrical stunning treatment as indicated by the appearance of protein bands at around 109 kDa and do not appear on other treatments. The difference is predicted from particular protein expressed induced by cell metabolism responsive in chicken liver cell.

KARAKTERISASI PRODUK BIOSOLUBILISASI LIGNIT OLEH KAPANG INDIGENUS DARI TANAH PERTAMBANGAN BATUBARA DI SUMATERA SELATAN Sugoro, Irawan; Hermanto, Sandra; Sasongko, Dwiwahju; Indriani, Dea; Aditiawati, Pingkan
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3116

ABSTRACTCharacterization of Lignite Biosolubilization Products by Indigenous Moulds from Soil ofCoal Mining in South Sumatera. Biosolubilization of coal is a potential technology of convertingsolid coal to liquid fuel and chemicals at ambient condition. Our previous research hassuccessfully isolated four moulds from soil at coal mining - South Sumatera and has potency aslignite biosolubilization agent, i.e. T1, T2, T4, T5. The objective of this research was to characterizeof lignite biosolubilization products by four isolates. The method used was sub-mergedculture. Cultivation medium was MSS+ (minimal salt + sucrose 0,1% + yeast extract 0,01% +lignite 5 %). Incubation was conducted at room temperature for 28 days. The result showed thatall indigenos moulds have different ability in lignite biosolubilization. The highestbiosolubilization occurred after 7 days of incubation belonging to T1 isolate. However, GC-MSanalysis showed the largest percentage of hydrocarbon compound which equivalent to gasolineand diesel was T5 after 7 days of incubation.Key words: Biosolubilization, lignite, moulds, coal.

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