During extensive field surveys in the 2015 year, Eurycoma apiculata A.W. Benn has been found for the first time in the forest reserve of Kenegerian Rumbio, Kampar District of Riau province. This finding is a new record of the medicinal plant in Indonesia. The taxonomic description and photographs of the species are given in this article.
The production of the double haploid plant in vitro through anther culture technique is a plant breeding technique used to obtain pure strain rapidly. A variety of pretreatment has been reported to induce callus and regenerate planlets efficiently. This study aims at describing the influence of cold anther pretreatment towards the callus formation of curly red chili pepper (Capsicum annuum L.). This research was conducted in the laboratory of Genetics and Breeding, Faculty of Agriculture and Animal Science, Universtas Islam Negeri Sultan Syarif Kasim Riau. The explants used are anther of local genotype of curly red chili pepper. The anthers are stored at low temperatures (4 Â°c) with different time intervals of 0, 24, 48 and 72 hours. The results showed that the percentage of highest callus formation was obtained at 24 and 72 hours length storage, ie 50%. Cold pretreatment of 72 hours anther storage results in a faster callus with a percentage of the highest yellowish white callus color of 17.65% and a compact structure. The cold pretreatment with 72 hours anther storage is the most optimal acceleration in the development stage of anther culture and induces te formation of curly red chili pepper (Capsicum annuum L.) local genotypes.
Eurycoma longifolia Jack is one of the extensively exploited medicinal plants in Indonesia. The objectives of this study were to obtain information on genetic diversity and population genetic structure of E. longifolia to formulate effective conservation plan. RAPD marker was used to assess the genetic diversity of E. longifolia collected from 5 natural populations in Riau Province. A total of 25 plants were analyzed using 5 RAPD primers, which amplified produced 44 scored DNA bands. The mean observed number of alleles per locus (No), number of effective alleles (Ne), and percentage of polymorphic loci (PPL) of E. longifolia were 1.57, 1.34, and 56.80%, respectively. The degree of differentiation among populations of E. longifolia was 0.31 (Ht = 0.29; Hs = 0.20). The mean value of estimated gene flow among populations of E. longifolia was 1.11 individual per generation. The UPGMA dendogram formed 2 significant clusters. The first cluster consisted of Pelalawan and Kampar populations, while the second cluster was formed from Kuansing, Rohul, and Rohil population. The genetic diversity information in this study is very important to perform efficient conservation and effective future management of its genetic resources.
Herbal and aromatic plants are attracting more attention among contemporary plant researchers because some human diseases resulting from bacterial antibiotic resistances have gained worldwide concern. Tropical forests in Kampar district were harboring potential plants genetic resources for herbal medicine, that it was economically potential to develop the next future. Presently, the information on potential plants genetic resources for herbal medicine is unknown. The objective of this research was to explore the plants genetic resources of herbal medicine in Kampar district. The result of exploration was found 32 species potential for herbal medicinal and aromatic. Most of them have been cultivated by society as house garden, but partially growth in nature wild. Local people have used as traditional medicine in decade. A part of potential plant was found difficult due to occur the heavy harvesting by society and conservation required
Forest of the UIN SUSKA Riau was harboring most of the genetic resources, one of them is pitcher plants (Nepenthes spp). Pitcher plants have high economic value because of they were ornamental and medicinal plants. This research aims to determine population density, diversity index, evenness index and dominance index of Nepenthes in the campus forest of UIN SUSKA Riau. This research used method was purposive sampling with square Jalur method. The result of this study found two species of Nepenthes in the campus of UIN SUSKA Riau, namely Nepenthes gracillis and Nepenthes ampularia. The average of population density of Nepenthes gracillis and Nepenthes ampularia were 2000 individuals/ha and 200 individuals/ha, respectively. The Diversity index of Nepenthes in this study was low (H’ <1), while average of evenness index (E) of Nepenthes was 0.352.
Rice is a cereal crop that belongs to the graminae family of high economic value, and is the main food for more than a portion of the world's population. This study aims to determine the relationship genetic of rice in Kampar by RAPD marker. Eight genotypes of rice from district in Kampar were amplified using eight primers (OPA 5, OPB 7, OPC 19, OPD 2, OPD 3, OPD 8, OPD 11, and OPD 13). The analysis of molecular data was performed by using NTSys pc -2.02 and GenAlex 6.1 . The results of the amplification of rice produced 48 loci with DNA band sizes obtained ranging from 350-1700 bp. The average percentage of polymorphic loci of the eight primers was 49.80%, where the highest percentage of polymorphic loci produced by OPA-7 primer was 75%, while the lowest polymorphic locus percentage was observed in OPD-13 primer which was 14.28%. The value of genetic distance between rice from Kampar in this study is in the range of 0.06 - 0.37. At a genetic distance of 0.79, rice plants can be grouped into two groups, namely the first group consisting of sokan and coku. The second group consisted of suntiong, korean , cupak putio, kuniong, jangguik, and cupak tenggi. The results of this study are expected to be taken into consideration in developing rice breeding strategies in the future.
Pitcher plant (Nepenthes sp) is a member of Nepenthaceae family. In Riau Province, pitcher plant is known locally as Periuk monyet. This species is economically important due to used as ornamental and medicine plants. In the present study, we want to know the kind of pitcher plant in peat swamp forest of State Islamic University of Sultan Syarif Kasim (UIN SUSKA) Riau. The result of exploration found that there are four species of Nepenthes growth in peat swamp forest of UIN SUSKA Riau, namely: N. ampularia, N. gracillis, N. mirabilis, and one species is estimated hybrid between N. ampularia x N. gracillis (Nepenthes x trichocharpa). The number of hybrid between N. ampularia x N. gracillis is found restricted, so that it is required another study to ensure that phenomenon.
Chili is an important horticultural plant in Indonesia. This research aims to carry out RAPD analysis on Mutant M2 of chili pepper (Capsicum annuum L.). Six M2 genotypes of chili irradiated by gamma ray and control plants were amplified by 16 random primers. The amplification results of M2 chili with 16 primers produced 118 loci, with fragment sizes ranging from 150-2000 bp. The number of polymorphic loci was 96 loci and the percentage of polymorphic loci was 83.23%. The DNA fragment polymorphism produced in this research was relatively high and it showed that the gamma ray mutagen applied produced high chili genetic diversity. The value of genetic similarity between control plants and mutant plants ranged from 0.7474 to 0.4874. UPGMA dendogram classified seven genotypes tested into three groups, the first group consisted of mutants R2U6 and R2U17, the second group was mutants R1U14 and R1U17, and the third group was mutants R2U8, mutants R2U2 and control plants. The finding of this research can be used as a basic selection of genetic material for chili’s breeding in the future.
The main obstacles in the commercialization of seedlings mass propagation through tissue culture techniques is the high cost of the culture media component. therefore, the production of lowcost tissue culture is required. This study aims to develop low cost in-vitro media for the production of the seedlings of Barangan banana. The research was composed following Factorial Completely Randomized Design, the first factor was Terra Novalgro liquid fertilizer with three concentrations, namely 1, 2, and 3 ml L-1, while the second factor was Gandasil with 3 concentrations namely 1, 2, and 3 mg L-1, so obtained nine treatments, each treatment was repeated 10 times, so that there were 90 experimental units. MS media was used as control. The parameter observed was number of shoots, number of leaves and number of roots. The results of this study exhibited that the treatment of 1 ml L-1 liquid fertilizer + 2 mg L-1 foliar fertilizer produced 9.30 shoots/explant and 1.90 leaves/explant, that no significantly different from MS medium (control) which produced 9.0 shoots/explant and 0.3 leaves/explants. Therefore, the using completed liquid fertilizers and foliar fertilizers as medium in vitro propagation of barangan bananas can become an alternative replace MS medium, as well as reduced the cost of culture media as 91% -93% compared to MS media.
Genetic diversity is an important aspect for plant to adapt on environment changes. Information on genetic diversity at individual, species and population levels are required for a basic consideration to formulate strategy conservation and breeding program and utilizing of genetic resources. In the last few decades, the development of DNA based molecular markers techniques has been a significant increase and its are routinely being used in ecological, evolutionary, taxonomical, phylogenetic, population genetic, identification studies of plant sciences. These DNA based markers are distinguished in two types, first non PCR based techniques such as RFLP (Restriction Fragment Length Polymorphism) and second is PCR based techniques such as RAPD (Random Amplified Polymorphic DNA), SSR (Simple Sequence Repeats) and AFLP (Amplified Fragment Length Polymorphism), Cleaved Amplified Polymorphic Sequences (CAPS), Sequence Characterized Amplified Region (SCAR), Single-Strand Conformation Polymorphism (SSCP) and DNA barcoding. These techniques are well established and their advantages as well as limitations have been realized. The review details account of techniques used in identification of markers and their applicability in plant sciences.