Garuda - Garba Rujukan Digital

The residue of trenbolone from male Garut sheep which implanted by trenbolone acetate R Widiastuti; Indraningsih .; T.B Murdiarti; R Firmansyah
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 3 (2001): SEPTEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Trenbolone acetate (TBA) is a growth hormone promoter which is implanted into animal to increase the body weight. The implantation of TBA in animal may cause the occurrence of residues of TBA and its metabolite (17-β-trenbolone). The presence of the residue might threat to human health. The aim of this research work was to study the presence of the residue of trenbolone in male Garut sheep which is implanted by TBA. The sheep were divided into 2 groups, those were D1 which were implanted by 40 mg TBA and D2 which were implanted by 60 mg TBA. One animal each from D1 and D2 were killed in the week 1, 2, 3 and 4 after implantation. And 24 hours before slaughtering the urine of D1 and D2 were collected using the metabolism cages. The samples for residue analysis were the organs such as liver, kidney, and the muscles around the implantation sites i.e inner and outer, upper front leg and upper back leg. The samples were extracted by organic solvents and analysed by HPLC. The residue of trenbolone occured in all samples of D1 and D2 which killed on 1 and 2 weeks after implantation. The residues occured on those which were killed 3 weeks after implantation were only in inner and outer samples. The residues in meat and organs were not detected in D1 nor D2 which were killed 4 weeks after implantation. The residue of trenbolone was also detected in the urine and still were detected in D1 and D2 which implanted by TBA for 4 weeks. Key words : trenbolone acetate, 17-β-trenbolone, residue, sheep

Trenbolone residue in tissues and urine of Onggole male calves treated with acetate trenbolone implant R Widiastuti; R Firmansyah; Indraningsih .
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Trenbolone acetate (TBA) is a hormone being permitted to be used as growth promoters for livestocks in several meat-exporting countries. The presence of trenbolone residu in animal products might affect human health. The purpose of this study was to determine the distribution of trenbolone residue (TBA dan 17 ß-trenbolone) in tissues and urine of Onggole male calves. The implantation of 200 mg TBA as Finaplix-H® was done subcutaneously on the back side of the medial part of ear. Urine were collected periodically until 21 days post implantation. The animals were terminated on the day 21st post implantation. Urine, meat and organs were analysed for trenbolone residues. The results showed that TBA residues were detected in tissues of inner and surrounding areas of the implantation sites and liver with an average concentration of 11 ng/g, 2,1 ng/g and 1,6 ng/g respectively. The 17 ß-trenbolone residue was only detected in tissue of inner area of the implantation site at the average concentration of 8,2 ng/g. Meanwhile, none of the residues were detected in urine. Key Words: Residue, Trenbolone, Implantation

Detection of deoxynivalenol and T-2 toxin in feeds by gas chromatography with Electron Captured Detector R. Widiastuti; Indraningsih .
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Deoxynivalenol (DON) and T-2 toxin are trichothecene mycotoxins produced by Fusarium spp. may pose to threat animal health. This research was aimed to validate an analysis method for DON and T-2 toxin detection in feed using a gas chromatography with electron captured detector ECD (GC-ECD) and to study the contamination level of DON and T-2 toxin in feed. Samples were extracted with organic solvents and derivitized with DMAP and HFBA prior to the detection with GC. Both toxins can be detected simultaneously with satisfied validation with GC. Analysis for DON showed that 17 out of 24 corn samples were positive (0.450 to 1.126 mg/kg), none was positive for 20 rice husk samples and 1 out of 14 cattle feed samples was positive (0.043 mg/kg), whereas analysis for T-2 toxin showed that 2 out of 24 corn samples were positive (0.022 and 0.063 mg/kg), 14 out of 20 rice husk samples were positive (0.027 to 0.720 mg/kg) and 6 out of 14 cattle feed samples were positive (0.039 to 0.084 mg/kg). The study showed that DON and T-2 toxin can be detected using GC-ECD. Whereas, the analysis results from field samples showed that the feeds were still safe to be used for animal feed, however a routine surveillance is needed to minimize further potential hazards. Key words: Deoxynivalenol, T-2 toxin, Gas Chromatography, Feeds

Neuropathology of organophosphate poisoning in dairy cattle Yulvian Sani; Indraningsih .
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The purpose of this study is to investigate pathological changes in brain tissues of Frisien Holstein dairy cattle affected by organophosphate (OP). The study was directed to anticipate spongiform encephalopathy. Samples consisted of animal feeds, sera and brain tissues were collected from Lembang, West Java. Animal feeds (fodders and commercial feed) were collected directly from the dairy farms around Lembang. Sera (31 samples) were from dairy cattle owned by the local farmers and brain tissues were from the local animal slaughter house. Pesticide residues were analysed following a standard procedure using gas chromatography (GC). There was an interaction between pesticide residues in animal feeds, residue level of pesticides in sera and brain tissues to cause encephalopathy in dairy cattle. Pesticide contamination in animal feeds was regarded as the source of encephalopathy in dairy cattle. The total average of OP residues (16.8 ppb) were lower than organochlorines/OC (18.7 ppb) in fodder, showing that pesticides were originated from the contaminated soils. On the other hand, the total average of OP residues in commercial feeds (12.0 ppb), sera (85.6 ppb) and brain tissues (22.7 ppb) were higher than OC (1.8; 16.7; and 5.1 ppb). The OP appears more frequently used for dairy farm activity as insecticides. Histopathological examination for brain tissues of dairy cattle showed that most cattle were diagnosed as encephalopathy with microscopic changes of vacuolation, neuronal necrosis, chromatolysis of neurons and nucleolysis of neurons. The encephalopathy was confirmed in rats intoxicated with chlorpyrifos methyl as severe brain damage with spongiform-like lesions. Key Words: Encephalopathy, Organophosphates, Cattle

Organophosphate poisoning in Ongole cattle in Sukamandi Yulvian Sani; Indraningsih .
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An integrated farming system between rice and beef cattle was initiated in 2000 in Sukamandi, West Java. However, since 2002 some cattle were suffering from neurological and ophthamological signs, and some of them were found dead after consuming rice straws. Field studies showed that 6 out of 12 cattle were suffered from eye disorders such as blindness, corneal opacity and sereous lacrimation. Two of 6 cattle were severely affected. But, neurological signs were not found during field observation. Pesticide analysis in rice straws shows that both groups of pesticide, organochlorines (OP) and organophosphates (OP) were detected. Residues of OC were higher than OP as shown as 14.41 vs 2.84 ppm (before processing) for OC and 1.80 vs 0.0003 ppm (processed) for OP consisting aldrin, lindane, dieldrin, endosulfan and chlorpyrifos methyl. There was only OC detected in sera consisting lindane (0.6–37.6 ppb); heptachlor (0.03–4.4 ppb); and aldrin (0.8–20.4 ppb) with an average total of 21,4 ppb. The neurological and ophthalmological signs were suspected to be due to organophosphates (OPs) contaminated-rice straws and any other factors such as insufficient vitamin A, magnesium and calcium. Feed replacement with fresh grasses for two cattle reduced corneal changes. Similar symptoms were also reproduced in Wistar rats dosed intraperitoneally with chlorpyriphos methyl. Clinical signs included incoordination, cachexia, eye disorder and corneal opacity. Necropsy showed hepatic injury (hepatic necrosis, mottling of hepatic surface and pale); pale kidneys; swollen of spleen and hyperaemic brain. Microscopic changes were found in brain, liver and eyes. Hepatic changes included haemorrhages, centrolobular hepatic necrosis and focal coagulative necrosis. Brains were showing focal necrosis, haemorrhages, vacuolisation, neuronal necrosis, chromatolysis and nucleolysis. Eyes appeared to have necrosis of tunica muscularis, haemorrhages and eosinophilic infiltration. The study indicates that organophosphates contamination in rice straws as animal feed may lead chronic neurological and ophthalmological symptoms. Key Words: Contamination, Pathology, Organophosphates, Rice Straws, Cattle

Pesticide Residues in Animal Products: Problems and its Alternative Prevention Indraningsih .; Yulvian Sani
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 14, No 1 (2004): MARCH 2004
Publisher : Indonesian Center for Animal Research and Development

Pesticides as agrochemicals are used to control plant diseases, however the use of pesticides may cause poisoning for human health and non-target animals, residual formation in animals and crops, and environmental contamination (soils and water). The impacts of pesticide are generally due to excessively or inappropriate use of pesticides. The surveillance on pesticide residues and/or contamination in animal products, crops and environment conducted in West Java and Lampung shows that some pesticide residues were detected in milk from West Java (Bogor and Pangalengan) including lindane, heptachlor, diazinon and endosulfan. In general, the pesticide residues in milk ofBogor were below the maximum residue limits (MRL), but two pesticide residues in milk of Pangalengan: diazinon (239 ppb) and heptachlor (60,3 ppb) appeared to be sufficiently high for human consumption. Some pesticide residues were also detected in meat, liver and fat of cattle in Bogor and Lampung. Both organochlorine and organophosphate residues were detected in samples of Bogor including: lindane (not detected/nd-135,5 ppb) and diazinon (nd-754,4 ppb) in meat; lindane (nd-16,7 ppb), diazinon (nd-969 ppb) and endosulfan (nd-191,8 ppb) in liver; and diazinon (nd-908,1 ppb) in fat. The residue level of diazinon detected in meat, liver and fat appeared to be higher than the MRL level. On the other hand, there was only organochlorine being detected in meat of Lampung. Regarding to pesticide residues being detected in soils, the source of contamination was then suspected from the contaminated soils where fodders being grown (grass, cabbages and corn). By-products of cabbage and corn are commonly used as animal feed for dairy and beef cattle respectively. The organic fanning system can be used as an alternative to minimise agricultural land contamination and pesticide residues in agricultural products. The low pesticide residues in agricultural by-products were able to reduce pesticide residues in animal products (meat and milk). The possibility of pesticide contamination should be taken into account in using agricultural by-products for animal feed to prevent pesticide residual effects in animal products. Key words: Residue, pesticides, minimalization, animal, organic

Review on Sources and Handling Method of Pesticide Residues in Animal Products Indraningsih .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development

Field studies and literature search showed that some pesticide residues either organochlorines (OC) or organophosphates (OP) were detected in animal products (meat and milk) . Pesticide residues in meat collected from West Java were detected at the level of 0 .8 ppb lindane and 62 ppb diazinon . While in meat from Lampung was detected at the level of 7 ppb lindane . 2 .7 heptachlor, 0 .8 endosulfan and 0 .5 ppb aldrin . Furthermore, pesticide residues were also detected in the milk collected from West, Central and East Java . The levels of lindane were 2,3 ; 15,9 ; 0,2 ppb ; heptachlor 8 ; 0 .4 and 0,05 ppb; diazinon 8 ; 0 and 1,8 ppb; CPM 0,4 ; 0,8 and 0 ppb ; endosulfan 0,1 ; 0,04 and 0,05 ppb for West, Central and East Java, respectively . The source of pesticide contamination in animal products is generally originated from feed materials, fodders . contaminated soils and water around the farm areas . Minimalization approach of pesticide residues in animal products could be conducted integratedly, such as through chemical process, biodegradation using microorganisms . Organic farming system is recognised as an alternative that may be applied to minimise contamination on agricultural land, eventually reducing pesticide residues in the agricultural products . Feeding with organic agricultural by-products with low pesticide residues appears to reduce pesticide residues in animal products . In order to eliminate pesticide contamination in soil, it has to be conducted progressively by implementing sustainable organic farming . Key words : Pesticide residues, meat, milk

Food Safety for Animal Products: The Need in The International Free Trade Sjamsul Bahri; Indraningsih .; R Widiastuti; T B Murdiati; R Maryam
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 12, No 2 (2002)
Publisher : Indonesian Center for Animal Research and Development

Issues of food safety, includes the food safety for the animal products grows to inhibit the incoming of low quality imported animal product which is unsafe for human being. Therefore several countries enhance to produce high quality of animal product and safe to be consumed. This paper reviews several factors that distribute to the quality and food safety in every single chain of the preparation of animal products. In pre-production step, many factors involve to the quality and safety because several contaminants and diseases can be happened in this step. Several data of diseases and contaminants which causes the negative impacts on economic, political and health will be provided in this paper. An effort to produce high quality and safe animal products can be done by implementing the HACCP in every step of preparation of it (GAP, GHP, GMP, GDP, and GRP). To assure the distribution of high quality and halal of imported animal products in Indonesia, it is necessary to give more strict regulation which is supported by infrastructure, facilities, human resources development and law enforcement. Key words: Food safety, animal products, international free trade, HACCP

The Use and Effect of Carbamate Insecticide on Animal Health and Products Indraningsih .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 18, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development

The use of carbamate in Indonesia is relatively new, in particular after prohibition on the use of most organochlorines (OC). Carbamates that commonly used for agricultural activities are carbofuran (Furadan), aldicarb (Temik) and carbaryl (Sevin). When properly used, they will provide benefit, but misuse of insecticides would affect productivity, poisoning, public health problems, environmental contamination and residues in foods. A monitoring result of carbamate used in Java indicates that carbofurans were detected in soils (0,8 – 56,3 ppb); water (0,1 – 5,0 ppb); rice (nd – 5,0 ppb); soybeans (1,2 – 610 ppb); animal feed (12 – 102 ppb); beef (110 – 269 ppb); and sera of beef cattle (167 – 721 ppb). The residue level was above the maximum residue limits (MRL) released by Indonesian Standardization Agency (Badan Standardisasi Nasional) in some samples. The presence of carbofuran in foods should be taken into account since the carbofuran is regarded highly toxic for public and animal health. This paper describes the toxicity of carbamate, clinical signs of poisoning, residue in foods and environment, handling of poisoning and residue control. Key Words: Carbamate, toxicity, residue, food, environment

Factors Affecting Food Safety of Animal Origin in Indonesia Sjamsul Bahri; Yulvian Sani; Indraningsih .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development

Based on the Indonesian Regulation Number 7 year 1996, food is defined as everything derived from biological sources and water, either processed and non-processed materials to be used as food and drinks, including food additives, food raw materials and other materials for preparation process, processing and production of food or drinks . Furthermore, food safety is a condition and an approach required to prevent the food from contamination of pathogenic microbes, toxic compounds and other xenobiotics that may affect and hazardous to human health . Food safety is basically a complexity and close related to policy . toxicity, microbiology, chemicals, nutrition status, health and public welfare . On the other hand, food safety problem is a dynamic process following the changing of public society including socioculture, health, development of science and technology as well as everything related to human life . In general, there are three main stages of process as the critical point in food safety of animal origin, namely : (1) preharvest ; (2) production ; and (3) postharvest . The main factors of these stages are discussed in this paper . Key words : Food safety, livestock, preharvest, harvest, post harvest

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