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All Journal The Journal of Experimental Life Sciences (JELS) Journal of Enviromental Engineering and Sustainable Technology
Nunung Harijati
Universitas Brawijaya
Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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SELECTING SEEDING TECHNIQUES AND PLANT MEDIA TO PRODUCE GENERATION ZERO (G0) TUBERS OF PORANG (Amorphophallus muelleri Blume) Imaniah Bazlina Wardani; Nunung Harijati
Journal of Environmental Engineering and Sustainable Technology Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jeest.2020.007.01.3

Abstract

Provision of seedlings A. muelleri in bulk can be done using seeds. The problem is seeds A.muelleri will be die if planted directly in the field. That caused seeds losing competition with weeds. So  needs a treatment to grow the seeds until zero generation (G0) tubers is formed. The technique used to maximize the formation of G0 tubers A. muelleri is selection of seed types and planting media. There are 3 types of seeds used, namely R  (red fruit seeds), Y (yellow fruit seeds) and G (green fruit seeds) where each type has a different level of maturity. Meanwhile there are 4 types of planting media used, namely M1 (soil: husk charcoal), M2 (soil: cocopeat), M3 (soil: cocopeat: husk charcoal) and M4 (soil). Every R, Y and G seeds were planted on M1, M2, M3 and M4 media. The parameters observed were weight, thickness and diameter of the G0 tubers. The data obtained were analyzed with 5% ANOVA and if there were differences followed by the 5% Tukey test. The results showed that the best G0 tubers were produced from planting R type seeds on M3 (RM3) media weighing 3.63 grams but were not significantly different from the treatment of planting R type seeds on media M1 (RM1) which was weighing 3.06 grams.  
The Relationship of Chemical Compounds and Crystal Development in Porang Tuber (Amorphophallus muelleri Blume) Nurul Chairiyah; Nunung Harijati; Retno Mastuti
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.01

Abstract

Chemical compounds are presumed to interact with each other and potentially affect the crystal development of porang tubers. This study focused on glucomannan and calcium oxalate (CaOx) as chemical compounds found in porang. Crystal development was analyzed by microscopic observation. The tuber tissue was used for microscopic slides and was harvested two weeks before the plants lost their leaves, when the plants lost their leaves, and two weeks after the plants lost their leaves. The CaOx and glucomannan content was measured using a modified extraction method. The increasing of CaOx content tends to increase the number of CaOx crystals. The analysis showed that the crystal density could affect the increasing of tuber weight possibility. There was a development process in raphide crystals that showed by different sizes at three harvest times, which the others were not. The results also indicated that glucomannan might influence the crystal density and the CaOx content. It can be concluded that glucomannan can potentially induce CaOx synthesis and crystal count increase. Keywords: Caox, Crystal, Development, Glucomannan, Interact, Porang.
The Study of Growth and Its Polyembryonic Properties of Porang Seeds (Amorphophallus muelleri Blume) from Various Fruit Colors in Different Planting Media Imaniah Bazlina Wardani; Nunung Harijati; Retno Mastuti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1199.582 KB) | DOI: 10.21776/ub.jels.2019.009.02.10

Abstract

The ripening of Porang compound fruit (Amorphophallus muelleri Blume) in one cob does not occur simultaneously but moves forward from the distal region to proximal. The ripe fruit is red, almost ripe is yellow and unripe is green. The study aimed to examine the growth potential and polyembryonic properties of Porang seeds derived from red, yellow and green fruits. These seeds were grown in different media with the expectation the best plant growth can be obtained. This research was a factorial experiment which was arranged in a completely random design and each unit observation has three replication. The factor I was the source of seeds. Seeds came from various fruit colors. Each of them is red (R), yellow (Y) and green (G). Factor II is a planting medium which had 4 types, M1, M2, M3, and M4.  M1 is combination of soil and husk charcoal (70%: 30%), M2 = soil: cocopeat (70%: 30%), M3 = soil: husk charcoal: cocopeat (70%: 15%: 15%) and M4 = just soil (100%). The results showed that the seeds derived from red fruit and planted on medium 2 (soil: cocopeat = 70%: 25%) potentially producing the best plant. The seeds also showed 100% polyembryony at 182 days after planting. In addition, the first, second and third shoots (originating from one seed) have different in height, canopy diameters, and petiole diameter. Keywords: Fruitcolors, planting media, polyembriony, Porang (Amorphophallus muelleri Blume).
Co-Authors Imaniah Bazlina Wardani Nurul Chairiyah Retno Mastuti