I Nyoman Pugeg Aryantha
Kelompok Keahlian Mikrobiologi-Genetika-Biologi Molekuler, Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung

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Penanda Molekul DNA Mikrosatelit untuk Karakterisasi Bibit Jamur Kuping (Auricularia polytricha [Mont.] Sacc.) I Nyoman Pugeg Aryantha; Yuniar Mulyani; Rahmat Ariffudin
Jurnal Matematika & Sains Vol 13, No 1 (2008)
Publisher : Institut Teknologi Bandung

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This study aims to find out a method of spawn characterization for wood ear mushroom (A. polytricha [Mont.] Sacc.)based on microsatellite DNA marker. Four strains of wood ear mushroom cultivated in several regions of Java i.e AUC(West Java), AUCN (Central Java), AUP (West Java) and AUT (West Java) were evaluated. A pair of designed primers(5’ -GGGAAAGTGATCCCATCTT-3’ and 3’-AGTTGTGGGAACATCGAACT-5’) was able to amplify microsatelliteDNA from those four strains of wood ear mushroom. Strains AUC, AUCN and AUP share the same positions (2 loci) ofmicrosatellite motive (TC)n with PCR products of 212 and 201 bp, while the same motive for strain AUT was found atdifferent loci with PCR products of 127, 108, and 96 bp. The same motive was also found at another locus of strainAUC and AUP with a PCR product of 228 bp. Another different locus with a PCR product of 85 bp also gave positiveamplification result for strain AUC. The data on macroscopic characters (mycelial growth rate and quality, fruitingpercentage and mushroom yield) revealed that AUC, AUCN and AUP are good strains, while AUT is bad strain.Overall, there is a good correlation between molecular (microsatellite DNA) patterns and macroscopic data on mycelialgrowth rate and quality, as well as flushing percentage and mushroom yield. Therefore, microsatellite DNA motive of(TC)n can be adopted for spawn characterization of wood ear mushroom.
Assessment of double screening programmes via solid substrate fermentation (SSF) in a flask system and identification of lovastatin potential producer Syarifah A. Rashid; Darah Ibrahim; I Nyoman Pugeg Aryantha
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Local economical substrates namely rice bran and unprocessed brown rice was applied into fermentation condition to produce a potent secondary metabolite compound, lovastatin. A basis condition of fermentation viz. 70% (v/w) of moisture content (adjusted to pH 6.0), 1x107 spore/ml of inoculum size, mixture of 1:1 substrates and 7 days of incubation period, was applied into SSF system. During a preliminary test, all of 72 fungi disclosed positive dark spot onto the thin layer chromatography plate (TLC). In order to verify the existence of lovastatin, the secondary screening which involving high performance liquid chromatography (HPLC) was conducted. Out of 72, only 71 fungi were detected as lovastatin producers and the highest production was stated from SAR I isolate with 68.72±0.84 mg lovastatin/g dry substrate and 0.87±0.03 mg glucosamine/g dry substrate of fungal growth. SAR I isolate was identified via colony and microscopic morphologies. Through the observations, SAR I isolate was identical to Aspergillus niger
Development of Simple-Sequence Repeats Markers from Durian (Durio zibethinus Murr. cultv. Matahari) Genomic Library Panca Jarot Santoso; Adi Pancoro; Sony Suhandono; I Nyoman Pugeg Aryantha
AGRIVITA, Journal of Agricultural Science Vol 39, No 3 (2017): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i3.1171


Simple sequence repeats have been proved as powerful markers and widely used in molecular breeding to reduce cycles and cost efective. The availability of the marker is, however, very limited in durian. This research aimed to develop SSR markers from durian genomic library. Genomic DNA was isolated from durian shoot leaf, whilst SSR motifs were isolated using membrane-based oligonucleotide enrichment hybridization protocol. Annotation made on the library found 527 unique motifs from 354 durian libraries which form 425 loci. The SSR motifs obtained were generally short repeats which reached 89.6 %, whilst longer repeats were found consisted of compound motifs. Eleven loci were selected as representative for further test to prove their informativity. A number of unique allels were successfully amplified from 17 durian genomes. The analysis showed the polymorphic information content (PIC) values ranged from 0.000 to 0.662 with an average of 0.390. The SSR loci also showed their ability to be used for durian diversity analysis as the evident that the loci could be used as genetic markers for assisting further durian breeding program.
Diversity of Culturable Bacterial in Various Parts of Luwak’s (Paradoxurus hermaprodithus javanica) Gastrointestinal Tract SONY SUHANDONO; HERI SETIADI; TATI KRISTIANTI; ALI BUDHI KUSUMA; ANDINI WARIH WEDARINGTYAS; DEMI TRISTAN DJAJADI; I NYOMAN PUGEG ARYANTHA
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (622.379 KB) | DOI: 10.5454/mi.10.2.4


Luwak coffee is a highly-priced coffee produced exclusively by the palm civet or luwak (Paradoxurus hermaphrodites ssp.). The purpose of this study was to determine the diversity of culturable bacteria in the gastro intestinal tract of luwak. The bacterial isolates were phenotypically characterized by their morphology and molecularly by analysis of their1,500bp 16s rDNA sequence. The results showed that Enterobacter cloacae and Lactobacillus brevis were found all over luwak’s digestive tract. Enterobacter cloacae was the most common species. The most diverse bacterial population was found in small intestine. Seven bacterial generawere successfully identified from the small intestine and colon, compared to only five genera found in the stomach.