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All Journal The Journal of Pure and Applied Chemistry Research
Harold Eka Atmaja
Pharmacokinetic Laboratory, Faculty of Medicine Universitas Padjadjaran, Bandung, Indonesia
Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Medicine & Pharmacology

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Partial Validation of Ultra Performance Liquid Chromatography Method for Quantification of Isoniazid-Pyrazinamide in Human Samples Vycke Yunivita; Triana Nurul Meirina; Atu Purnama Dewi; Harold Eka Atmaja; Rovina Ruslami
The Journal of Pure and Applied Chemistry Research Vol 10, No 3 (2021): Edition September-December 2021
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2021.010.03.600

Abstract

Isoniazid (INH) and pyrazinamide (PZA) are first-line drugs in the treatment of meningitis of tuberculosis, in addition to rifampicin. The use of these drugs will determine the success of therapy to kill Mycobacterium tuberculosis in brain tissue that is difficult to penetrate by other drugs. Therefore, it is necessary to conduct research and monitor the use of this drug in plasma and cerebrospinal fluid (CSF) patients with TBM. This study aimed to determine the method of analysis of INH and PZA using Ultra Performance Liquid Chromatography Ultra Violet (UPLC-UV). The samples were taken from TBM patients who taken INH-PZA and others besides TBM patients who did not take INH-PZA. The analytic method carried out includes a comparison of the results of the analysis method for measuring levels of standard INH-PZA and INH-PZA in plasma and CSF samples. Analysis of INH-PZA in plasma and CSF can be performed using UPLC with UV detector, at least 100 uL plasma or CSF samples volume, with reproducible and accurate results.
A Validated Ultra Performance Liquid Chromatography Method for Quantification of Metformin in Human Plasma Vycke Yunivita Kusumah Dewi; Atu Purnama Dewi; Budi Setiabudiawan; Edhyana Sahiratmadja; Triana Nurul Meirina; Harold Eka Atmaja; Rovina Ruslami
The Journal of Pure and Applied Chemistry Research Vol 7, No 3 (2018): Edition September-December 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.45 KB) | DOI: 10.21776/ub.jpacr.2018.007.003.414

Abstract

Metformin is oral hypoglycemic or blood sugar-lowering drug which is used for the first- line drug in the treatment of diabetes mellitus type 2. This study presented the validated  Ultra Performance Liquid Chromatography UltraViolet (UPLC-UV) method for the determination of metformin in human plasma. Metformin levels were measured using UPLC with a UV detector and liquid-liquid extraction method. Separation was carried out on an Acquity UPLC HSS T3 100mm × 2.1mm i.d. column (1.8μm particle size) using gradient elution of acetonitrile and phosphate buffer 0.02 M (0.6 mL/min) as mobile phase at 30°C. The analyte was monitored at 236 nm. No endogenous substances were found to interfere with the peaks of drug and internal standard. The value of percent deviation and the coefficient variation obtained respectively less than the percentage set in the FDA guidelines. The linearity factor values were more than 0.997 and LOD was 0.01µg/mL. UPLC with UV detector is able to analyze metformin in a short time with good precision and accuracy which is useful for bioequivalence and bioavailability studies.
Co-Authors Atu Purnama Dewi Budi Setiabudiawan Edhyana Sahiratmadja Rovina Ruslami, Rovina Triana Nurul Meirina Vycke Yunivita Vycke Yunivita Kusumah Dewi