Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal The Journal of Pure and Applied Chemistry Research
Edhyana Sahiratmadja
Division of Biochemistry and Molecular Biology, Department of Biomedical Sciences, Faculty of Medicine Universitas Padjadjaran, Bandung, Indonesia
Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Medicine & Pharmacology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
A Validated Ultra Performance Liquid Chromatography Method for Quantification of Metformin in Human Plasma Vycke Yunivita Kusumah Dewi; Atu Purnama Dewi; Budi Setiabudiawan; Edhyana Sahiratmadja; Triana Nurul Meirina; Harold Eka Atmaja; Rovina Ruslami
The Journal of Pure and Applied Chemistry Research Vol 7, No 3 (2018): Edition September-December 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.45 KB) | DOI: 10.21776/ub.jpacr.2018.007.003.414

Abstract

Metformin is oral hypoglycemic or blood sugar-lowering drug which is used for the first- line drug in the treatment of diabetes mellitus type 2. This study presented the validated  Ultra Performance Liquid Chromatography UltraViolet (UPLC-UV) method for the determination of metformin in human plasma. Metformin levels were measured using UPLC with a UV detector and liquid-liquid extraction method. Separation was carried out on an Acquity UPLC HSS T3 100mm × 2.1mm i.d. column (1.8μm particle size) using gradient elution of acetonitrile and phosphate buffer 0.02 M (0.6 mL/min) as mobile phase at 30°C. The analyte was monitored at 236 nm. No endogenous substances were found to interfere with the peaks of drug and internal standard. The value of percent deviation and the coefficient variation obtained respectively less than the percentage set in the FDA guidelines. The linearity factor values were more than 0.997 and LOD was 0.01µg/mL. UPLC with UV detector is able to analyze metformin in a short time with good precision and accuracy which is useful for bioequivalence and bioavailability studies.
Co-Authors Atu Purnama Dewi Budi Setiabudiawan Harold Eka Atmaja Rovina Ruslami, Rovina Triana Nurul Meirina Vycke Yunivita Kusumah Dewi