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All Journal Jurnal Biologi Universitas Andalas Jurnal Biologi Tropis
Wahyu Aji Mahardhika
Diponegoro University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Characterization And Screening Of Protease, Amylase, And Cellulase From Phylloplane Fungi Isolates Of Avicennia marina (Forssk.) Vierh. Wahyu Aji Mahardhika; Warih Ramadhany; Arina Tri Lunggani
Jurnal Biologi Universitas Andalas Vol 9, No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.54-59.2021

Abstract

Mangrove (Avicennia marina) is an important mangrove species, because it has many benefits for human life, including in the ecological scope as a source of food and medicine. These plants can also be used as a source of symbiont mold isolates which can be developed as an alternative to produce bioactive compounds, one of which is enzymes. Enzymes are protein compounds that can catalyze all chemical reactions in biological systems. This study aims to determine whether mangrove symbiont fungi (A. marina) can produce amylase, protease and cellulase enzymes. This research was conducted using a method based on enzyme activity, namely amylase activity with lugol iodine staining, protease activity and cellulase activity with congo red staining. on agar media enriched with 1% skim milk, 1% starch and 1% CMC. The results showed that 4 isolates were able to show potential enzymatic activity to be developed as agents for enzyme production.
Isolation and Characterization of Mold on Furniture in Biological Laboratory Environment Using Contact Plate Method Wahyu Aji Mahardhika; Romario Dion; Mochammad Fa’iq Qoys Naufal; Warih Ramadhany; Arina Tri Lunggani
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.3416

Abstract

The presence of molds in the environment around us can be beneficial or detrimental. Mold can be found in various places, including soil, air, plants, animals, water, and on material surfaces. One of the roles of mold is as a bio-degradator and biodeterioration agent on materials that are still used, so that it will cause harm to humans. This study aims to isolate and characterize the types of molds that exist on the surface of existing materials in the biology laboratory environment of Diponegoro University with the contact plate method. The method used is to attach a contact plate containing PDA to various materials such as tables, chairs, cabinets, and so on, then observe the macroscopic and microscopic morphology. The results obtained were five genera, there are Aspergillus, Penicillium, Eurotium, Rhizopus, and Absidia.
Co-Authors Arina Tri Lunggani Arina Tri Lunggani Mochammad Fa’iq Qoys Naufal Romario Dion Warih Ramadhany Warih Ramadhany