Article Per Year (5 Year)
p-Index From 2019 - 2024
0.983
P-Index
This Author published in this journals
All Journal BERKALA SAINSTEK BioWallacea Journal of Biological Research JIPHO ( Jurnal Ilmiah Peternakan Halu Oleo) Indonesia Chimica Acta Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) JURNAL ILMU KEFARMASIAN INDONESIA
Ardiansyah Ardiansyah
Jurusan Biologi, Fakultas MIPA Universitas Halu Oleo Kendari.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Veterinary Other

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
Pengaruh Ekstrak Daun Tembelekan (Lantana camara) Terhadap Penampakan Histopatologi Thallus Rumput Laut (Kappaphycus alvarezii) Emand Syapriawan Tolanamy; Agusrinal Agusrinal; Ardiansyah Ardiansyah
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 7, No 2 (2020): Biodiversitas on Asian Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1064.259 KB) | DOI: 10.33772/biowallacea.v7i2.14593

Abstract

The purpose of the study was to analyze the ability of Lantana camara extract on preventing and decreasing the transmission ratio of bacteria causing ice-ice disease of seaweed thallus Kappaphycus alvarezii by cohabitation and to find out the difference of histopathology of the thallus. The effect of thallus submersion interaction and the growths were analyzed statistically. The results showed tembelekan leaf extract solution can inhibit the growth of the bacteria by cohabitation method. Submersion treatment for 60 minutes was able to suppress the ability of pathogens transmitting than 30 and 90 minutes by the cohabitation method. Pathologically, an 60 minutes submersion treatment shows better than any other treatments Abstrak Penelitian ini bertujuan menguji kemampuan larutan ekstrak daun tembelekan Lantana camara dalam mencegah dan mengurangi tingkat transmisi bakteri penyebab penyakit ice-ice pada thallus rumput laut Kappaphycus alvarezii secara kohabitasi, prevalensi secara in vivo dan melihat perbedaan pada thallus secara histopatologi. Hasil penelitian menunjukkan larutan ekstrak daun tembelekan dapat menghambat pertumbuhan bakteri  penyebab ice-ice secara kohabitasi. Perlakuan perendaman selama 60 menit lebih mampu menekan kemampuan transmisi bakteri pathogen dibandingkan selama 30 dan 60 menit. Dilihat dari penampakan secara histopatologi, perlakuan 60 menit perendaman memperlihatkan jaringan yang lebih baik dibandingkan dua perlakuan lainnya
Isolasi dan Seleksi Bakteri Pendegradasi Solar Dari Pelabuhan Penyeberangan Kendari – Wawonii, Sulawesi Tenggara M. Rajab Sutra Mijaya; Nur Arfa Yanti; Ardiansyah Ardiansyah; Nurhayani H. Muhiddin
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.347 KB) | DOI: 10.33772/biowallacea.v6i2.8825

Abstract

This study aimed to obtain the ability of bacteria to degrade diesel fuel. Method of this research was exploration method. Bacteria were isolated by enrichment method used SMSSe enriched diesel 2% (v/v). Selection of hidrocarbonoclastic bacteria based on the ability of to grow on agar medium solid. The bacteria in the test made in the form of suspension with Mc Farland Standard 0.5. Test of bacterial isolates were used 10% of the inoculum put in 150 mL media with different concentrations of diesel fuel were 1%, 2% and 3% and incubated on a rotary shaker at 120 rpm. Samples were taken on 1, 5, 10, 15 and 20 days to test diesel emulsion by centrifugation at a speed of 3500 rpm ±15 minutes, the comparison between the media and diesel 4:1. Growth in the amount of bacteria accounted by a Standard Plate Count method. The levels of the diesel rest calculated every sampling during incubation. The selection results of obtained diesel degrading bacteria isolates that PSI.1 PSII.1 and PSIII.2. All of bacteria have the ability to degrade diesel fuel in different treatment, the best result in lowered diesel fuel that were a concentration of 3% during 20 days of incubated at PSII.1 isolate, that have the highest ability to reduce levels of diesel up to 70,70%.Keywords: Hydrocarbonoclastic, Degradation, Diesel fuels ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan bakteri dalam mendegradasi solar. Penelitian ini adalah penelitian eksploratif. Isolasi bakteri menggunakan metode enrichment dengan media yang digunakan yaitu SMSSe yang diperkaya solar 2% (v/v). Pemilihan bakteri hidrokarbonoklastik berdasarkan kemampuan bakteri tumbuh pada media agar padat. Inokulum bakteri dibuat dalam bentuk suspensi dengan Standar Mc Farland 0,5. Pengujian kemampuan degradasi solar dilakukan menggunakan media minimal dengan variasi konsentrasi solar 1%, 2% dan 3% dan diinkubasi pada rotary shaker. Sampel diambil pada hari ke 1, 5, 10, 15 dan 20 untuk uji emulsi solar dengan menghitung volume solar yang teremulsi. Pertumbuhan jumlah bakteri dihitung dengan metode Standard Plate Count. Kadar sisa solar dihitung setiap pengambilan sampel selama inkubasi. Hasil seleksi bakteri pendegradasi solar diperoleh tiga isolat yaitu PSI.1 PSII.1 dan PSIII.2. Ketiga isolat bakteri memiliki kemampuan mendegradasi solar pada perlakuan yang berbeda, hasil terbaik dalam menurunkan kadar solar yaitu konsentrasi 3% selama 20 hari inkubasi pada isolat PSII.1 yang memiliki kemampuan tertinggi menurunkan kadar solar hingga 70,70%. Kata kunci : Hidrokarbonoklastik, Pendegradasi, Solar
Aktivitas Antibakteri Kombucha Daun Sirsak (Annona muricata L.) Dengan Konsentrasi Gula Berbeda Nur Arfa Yanti; Sri Ambardini; Ardiansyah Ardiansyah; Wa Ode Leni Marlina; Kartika Dwi Cahyanti
BERKALA SAINSTEK Vol 8 No 2 (2020)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v8i2.15968

Abstract

Kombucha daun sirsak merupakan minuman hasil fermentasi simbiosis antara bakteri asam asetat Acetobacter xylinum dan khamir Saccharomyces sp. yang menggunakan bahan baku rebusan daun sirsak. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kombucha daun sirsak dengan variasi konsentrasi gula yang berbeda. Konsentrasi gula yang digunakan dalam pembuatan kombucha daun sirsak adalah 10%, 20%, 30% dan 40% (b/v). Pengujian aktivitas antibakteri dilakukan dengan metode difusi sumuran menggunakan bakteri Escherichia coli dan Staphylococcus aureus. Kombucha daun sirsak memiliki aktivitas antibakteri dengan spektrum luas. Kombucha daun sirsak dengan konsentrasi gula 20% memiliki aktivitas antibakteri tertinggi. Dengan demikian, kombucha daun sirsak sangat berpotensi sebagai minuman kesehatan.
Isolasi Dan Karakterisasi α-amilase Isolat Bakteri Amilolitik Asidofilik Dari Taman Nasional Rawa Aopa Watumaohai Sapto Raharjo; Ardiansyah Ardiansyah; Agus Chahyadi
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 1 No 1 - December 2008
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v1i1.2451

Abstract

Isolation and characterization α-amylase acidophilic amylolytic bacteria isolate from Rawa Aopa Watumohai National Park has been carried out. The aims of these studies are to know the characteristics of acidophilic amylolytic bacteria isolate, the optimum concentration of substrate and temperature α-amylase activity yielded, and specific activity in each enzyme purification steps. Activity of α-amylase was measured with DNS (Dinitrosalisilic acidI) method at λ 550 nm and the rate of protein enzyme was measured with Biuret method at λ 540 nm. The content of this research were obtained the isolate bacteria TR 1 which higher amylolytic activity is the colonies are pale-yellow with convexelevated, undulate, bacill sel, and negative Gram. The maximum activity and protein content obtained with (NH4)2SO4 extracted at 60% (w/v). The specific activity of α-amylase in crude extract was 0.038 U/mg enzyme, after extracted with (NH4)2SO4 60% (w/v) was 0.146 U/mg enzyme with purification fold 3.8, and after dialysis was 0.255 U/mg enzyme with purification fold 3.8, respectively. The optimum concentration of substrate (amylose) was 1.25% (w/v) and the optimum incubation temperature was 45oC.Keywords : Rawa Aopa Watumohai National Park, acidophilic amylolytic bacteria, α-amilase
Isolasi dan Identifikasi Jatrophone dari Akar Jarak Merah (Jatropha gossyptfolia) serta Evaluasi Sifat Toksik dan Sitotoksiknya SAHIDIN I; ARDIANSYAH ARDIANSYAH; MUHAMAD TAHER
JURNAL ILMU KEFARMASIAN INDONESIA Vol 8 No 2 (2010): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1282.056 KB)

Abstract

Isolation and identitication of secondary metabolites from root of Jatropha gossypyolia, and also its biological activity test toward microbes and tumor cells have been conducted, Isolation was carried out by using vacuum liqiud chromatography (VLC) method (adsorbent: silica gel, eluent: mixture of n-hexane, ethylacetate, chloroform, and methanol). The isolate molecular structure Was determined by spectroscopic technique i.e. FTIR, NMR-ID, 13C-DEPT, NMR-2D, comparing the data obtained with related data from literatures. Its biological activity was evaluated against bacteria (Acetobacter sp., Escherecia coli, Staphylococcus aureus, and Streptococcus sp.), fungi (Aspergillus nigcr, Pentcillium sp. [grey], Penicillium sp. [White] and Rhizopus sp.), and murine leukemia P-388 cells. The results showed that the isolate is a white needle crystal which is identified as jatrophone. Jatrophone is very active toward Acetobacter sp. (bacteria) and A. niger (fungi) with growth inhibition Zone of 3,6 cm and 4.4 cm, respectively. Meanwhile, jatrophone has low activity against murine leukemia P-388 cells with IC50, > 100 µg/mL.
Inhibition of HMG-CoA Reductase Activity by Kersen Leaves (Muntingia calabura L.) to Prevent Hypercholesterolemia: Inhibisi HMG-CoA Reduktase Menggunakan Ekstrak Daun Kersen (Muntingia calabura L) Untuk Mencegah Hiperkolesterolemia Tien Tien; Nishar Rakhman Ardiansyah; Carla Wulandari Sabandar; Laode Kardin; Pranita Aritrina
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 9 No. 1 (2023): (March 2023)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2023.v9.i1.16086

Abstract

Background: Hypercholesterolemia is a condition of total cholesterol level >200 mg/dL and LDL >130 mg/dL. HMG-CoA (3-hydroxy-3-ethylglutaryl-coenzyme A) reductase is an enzyme that has a role in cholesterol biosynthesis. Hence, inhibition of this enzyme led to the decrement of cholesterol level. The extract of Kersen leaves (Muntingia calabura L.) is known to contain flavonoids, terpenoids, steroids, tannins, phenolic, and alkaloids. Flavonoids work by inhibiting the HMG-CoA reductase activity, so that mevalonate cannot be formed and thus decrease the cholesterol synthesis. Objectives: The present study aimed to determine the effect of Kersen leaves extract (M. calabura L.) in inhibiting the HMG-CoA reductase activity in vitro. Material and Methods: The study is a true experimental study with a post-test-only control group design. The independent variables were ethanol, methanol, and n-hexane extracts of Kersen leaves. Moreover, the percentage inhibition of the enzyme was the dependent variable. The test was conducted in vitro using UV-Vis spectrophotometry with pravastatin as a positive control. Results: The inhibitory effects of ethanol, methanol, n-hexane extracts of Kersen leaves, and pravastatin towards HMG-CoA reductase activity were 85.56%, 59.75%, 92.03%, and 99.58%, respectively. Post Hoc One-Way ANOVA showed that the p-values of pravastatin with ethanol, methanol, and n-hexane extracts were 0.687, 0.048, and 0.931, respectively. The n-hexane and ethanol extracts were potent for inhibiting the enzyme activity (p>0.05) comparable to pravastatin. Conclusion: The n-hexane and ethanol extracts of Kersen leaves could serve as a natural source of HMG-CoA reductase inhibitor to prevent hypercholesterolemia.
Karakteristik Fisiko Kimia Pelepah dan Daun Kelapa Sawit yang Diamoniasi dengan Larutan Urea-Molases Level Berbeda Muh Ardiansyah; La Malesi; Ali Bain
Jurnal Ilmiah Peternakan Halu Oleo Vol 5, No 2 (2023): JIPHO (Jurnal Ilmiah Peternakan Halu Oleo)
Publisher : Fakultas Peternakan Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56625/jipho.v5i2.35396

Abstract

Abstrak. Pelepah dan daun kelapa sawit merupakan limbah padat perkebunan kelapa sawit yang memiliki potensi untuk digunakan sebagai pakan basal ternak ruminansia, Penelitian ini bertujuan untuk mengevaluasi efektivitas amoniasi menggunakan larutan urea-molases yang berbeda dalam proses amoniasi terhadap warna, kadar protein kasar dan serat kasar pelepah dan daun kelapa sawit amoniasi.  Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) yang terdiri atas 4 perlakuan dan masing-masing perlakuan terdiri atas 4 ulangan sehingga terdapat 16 unit satuan percobaan.  Jenis perlakuan terdiri atas :  P1 (pelepah dan daun kelapa sawit tanpa amoniasi), P2 (pelepah dan daun kelapa sawit yang diamoniasi dengan  larutan urea 1% + molases 5%),  P3 (pelepah dan daun kelapa sawit yang diamoniasi dengan larutan urea 2% + molases 6%), P4 (pelepah dan daun kelapa sawit yang diamoniasi dengan larutan urea 3% + molases 7%).  Variabel yang diamati dalam penelitian ini adalah fisik yaitu (warna), kimia (protein kasar) dan (serat kasar).  Hasil penelitian menunjukkan bahwa amoniasi menggunakan larutan urea-molases mempengaruhi (P<0,05) warna, protein kasar dan serat kasar pelepah dan daun kelapa sawit.  Semakin tinggi pemberian dosis larutan urea-molases maka menghasilkan perubahan warna yang berbeda setiap perlakuan, peningkatan kadar protein kasar dan penurunan kadar serat kasar dari pelepah dan daun kelapa sawit yang diamoniasi. Dosis larutan urea-molases yang memberikan hasil terbaik adalah amoniasi dengan menggunakan larutan dengan dosis urea 2% + molases 6%.          Kata Kunci :  Pelepah dan daun kelapa  sawit, amoniasi, karakteristik fisik dan kimia.
Co-Authors Agus Chahyadi Agusrinal Ali Bain Carla Wulandari Sabandar Emand Syapriawan Tolanamy Haji Muhiddin, Nurhayani Kartika Dwi Cahyanti La Malesi Laode Kardin M. Rajab Sutra Mijaya MUHAMAD TAHER Nur Arfa Yanti Pranita Aritrina SAHIDIN I Sapto Raharjo Sri Ambardini Tien Tien Wa Ode Leni Marlina