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All Journal MPI (Media Pharmaceutica Indonesiana) ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Makara Journal of Science
Gunawan Indrayanto
Graduate Program, Faculty Of Pharmacy, University Of Surabaya, Surabaya
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Makara Journal of Science

 1 
Sterol and Triterpene Profiles of the Callus Culture of Solanum mammosum Juliana, Silvy; Suciati,; Indrayanto, Gunawan
Makara Journal of Science Vol. 23, No. 2
Publisher : UI Scholars Hub

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This study aimed to compare the sterol and triterpene profiles of two types of Solanum mammosum callus cultures , i.e., compact globular structure (CGS) and normal fine (F) calluses. The CGS callus resulted from the differentiation of the F calus culture after many years of subculturing. The growth rate, microscopic characteristics, and morphologies of the two callus types were determined and compared. Sterols and triterpenes were identified through thin-layer chromatog-raphy, gas chromatography-flame ionization detection, and gas chromatography–mass spectrometry analyses. The growth rate of the CGS callus was lower than that of the F callus. Microscopic identification revealed that thick, lignincontaining cell walls formed in the CGS callus but not in the F callus. The chromatographic analysis suggested that the CGS and F callus cultures had different sterol and triterpenoid profiles. The sterols and triterpenes produced by the CGC culture were more diverse than those produced by the F callus culture
Identification of Massoia Lactone and its Hydroxy-derivative from Kabatiella caulivora, an Endophyte of the Terrestrial Plant Alyxia reinwardtii Laili, Izzatul; Gunarso, Sri; Wathan, Nashrul; Sugijanto, Noor Erma; Indrayanto, Gunawan
Makara Journal of Science Vol. 21, No. 4
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Massoia lactone (1) and its derivative, 3,5-dihydroxydecanoic acid-d-lactone (2), were identified from an ethyl acetate extract of the endophytic fungus Kabatiella caulivora, isolated from its host Alyxia reinwardtii. The compounds were identified by GC-MS. To the best of our knowledge, this is the first report of the identification of massoia lactone and its derivative from the endophytic K. caulivora fungus.
Application of Accuracy and Precision Evaluations Based on the Current United States and Indonesian Pharmacopoeias: A Critical Review Indrayanto, Gunawan
Makara Journal of Science Vol. 26, No. 4
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New methods for the evaluation of accuracy and precision are mentioned in the latest edition of the United States Pharmacopoeia (USP), whereas other validation parameters, that is, selectivity, linearity, range, and robustness, remained relatively unchanged. In obtaining reliable data from any chemical/pharmaceutical analysis, the analytical procedure must be validated or verified in accordance with the latest edition of the pharmacopoeia. Some review articles on the general validation methods have been published by the author. This present review will focus on the implementation and discussion of the accuracy and precision evaluation based on the current USP and Indonesian pharmacopoeia. Some examples of the calculation of several accuracy and precision method of determinations are also discussed.
Isolation of Pandangolide 1 from Cladosporium oxysporum, An Endophyte of the Terrestrial Plant Alyxia reinwardtii Hartanti, Dwi; Purwanti, Diah Intan; Putro, Herdayanto Sulistyo; Rateb, Mostafa Ezzat; Wongso, Suwidji; Sugijanto, Noor Erma; Ebel, Rainer; Indrayanto, Gunawan
Makara Journal of Science Vol. 18, No. 4
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that the 2011 IOD was a weak and short-lived event. It developed in July, peaked in September, decayed in October and
Co-Authors Armila Fatma Setyaningrum Cece Furwanti Dwi Hartanti Ebel, Rainer Gunarso, Sri Juliana, Silvy Kusuma Hendrajaya Laili, Izzatul Motoo Tori Nashrul Wathan Noor Cholies Zaini Noor Erma Nasution Sugijanto Noor Erma Sugijanto Purwanti, Diah Intan Putro, Herdayanto Sulistyo Rateb, Mostafa Ezzat Risma Pratiwi Suciati Suciati SUCIATI, Suyatno Suyatno Wongso, Suwidji