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All Journal Jurnal Penelitian Hutan Tanaman Indonesian Journal of Biotechnology Jurnal Wana Tropika
Sapto Indrioko
Faculty of Forestry, Gadjah Mada University, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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PEROLEHAN GENETIK PADA UJI KLON JATI (Tectona Grandis L.F) UMUR 3 TAHUN DI KHDTK KEMAMPO, SUMATERA SELATAN Sofyan, Agus; Indrioko, Sapto
Jurnal Penelitian Hutan Tanaman Vol 8, No 3 (2011): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Peningkatan Produktivitas Hutan

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Paternity Analysis of Tea (Camellia sinensis L. Kuntz) Hybrids Using Isozyme Marker Setyorini, Titin; ., Taryono; ., Suyadi; Indrioko, Sapto
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Tea plant has been categorized as self-incompatible crop. This is the reason behind the high genetic diversity. Natural pollination is possible to occur and the male parent is usually unknown, therefore, there is a need of method to identify male parent of hybrids through paternity analysis. Isozyme markers have been successfully used for paternity analysis due to their co-dominant polymorphism. This research aimed to predict male parents of hybrids by figuring out the mating system through isozyme banding patterns. In this experiment, seven enzyme systems were evaluated, of which only two of the enzyme systems i.e. esterase and shikimate dehydrogenase showing clear band pattern of Est-1, Est-2, and Shd-1 loci. The mating system of tea could be categorized as a mixed mating model, with high estimated out-crossing rate of 98.6 %. The pollen contributors were not always originated from the vicinity of the female parents.Key words: isozyme markers, paternity analysis, tea
Allozyme variation of the endemic and vulnerable Dyera lowii Hook.f. in Central Kalimantan: Implications for genetic resources conservation Wahyudiningsih, Tri Suwarni; Naiem, Mohammad; Indrioko, Sapto; Sumardi, Issirep
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found inpeat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existenceis now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity withinfour natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in CentralKalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectricfocusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Meannumber of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Geneticdifferentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozymevariation was found within population (93.2%). Special attention is essential to conserve a private allele ofGot-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as foundin other populations. Selat Nusa population is expected to enhance the effective management for geneticresources conservation of this vulnerable species in the future.
EVALUASI DAMPAK PROGRAM BENIH UNTUK RAKYAT (SEED FOR PEOPLE) DI DESA KASEGERAN, KECAMATAN CILONGOK, KABUPATEN BANYUMAS Farobi, Ibnu; Na'iem, Mohammad; Sabarnurrdin, Sambas; Indrioko, Sapto
Jurnal Wana Tropika Vol 5 No 2 (2015)
Publisher : Fakultas Kehutanan Institut Pertanian STIPER Yogyakarta

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Co-Authors Agus Sofyan Farobi, Ibnu Issirep Sumardi Mohammad Naiem Na'iem, Mohammad R. Sapto Hendri Boedi Soesatyo Sabarnurrdin, Sambas Suyadi . Titin Setyorini, Titin Tri Suwarni Wahyudiningsih