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Genetic Relationship between Tongka Langit Bananas (Musa troglodytarum L.) from Galunggung and Maluku, Indonesia, Based on ITS2 Fenny Martha Dwivany; Giasintha Stefani; Agus Sutanto; Husna Nugrahapraja; Ketut Wikantika; Adriana Hiariej; Topik Hidayat; I Nyoman Rai; Nisrina Sukriandi
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.258

Tongka Langit or Fe’i banana (Musa troglodytarum L.) has the T genome and a very high content of beta-carotene. It only grew and spread around the regions of Maluku islands and Papua. However, recently our team found this banana on the foot of mount Galunggung, West Java, so this raised the question about its origin. The objective of this study was to understand the genetic relationship between Tongka Langit from Galunggung and Maluku islands and compared it with other bananas with different genomes. Genetic diversity analysis was done using ITS2 DNA marker and dendrogram analysis showed three groups. From the comparison of the ITS2 sequences, there were no difference (100% identity) between the ITS2 sequence of Tongka Langit originating from Galunggung and Maluku. In conclusion, based on the ITS2 marker, the Tongka Langit were more distantly related to cultivars with A and B genomes, and there was no difference in the ITS2 sequence of Tongka Langit originating from Galunggung and Maluku. To the best of our knowledge, there is no previous report of genetic relationship between Tongka Langit from Galunggung and other regions.

Development Of Appropriate Technology Adoption For Coffee Farmers In Sukawangi Village, Sumedang Bentang Arief Budiman; Hari Purnama; Husna Nugrahapraja; Muhammad Abdur Rasyid; Natas Setiabudhi Daryono Putra; Sri Aswin Suryani; Annisa Ratna Nurillah; Ima Mulyama Zainuddin; Rindia Maharani Putri; Wervyan Shalannanda; Raden Aswin Rahadi
Jurnal Ilmu Sosial Politik dan Humaniora (Jisora) Vol 2 No 2 (2019): Jurnal Ilmu Sosial Politik dan Humaniora
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik Universitas Garut

In this paper, the authors propose solutions to increase the added value of coffee in the form of the process of making the process of making coffee using appropriate technology adoption. This paper uses qualitative methods of locational survey and literature study. Survey activities are conducted in the form of interviews and observations. The team conducted interviews with coffee farming communities in the area and observed the conditions of coffee farming. The literature study was conducted by synthesizing literature reviews and continued by formulating the theory and principles of appropriate technology in the context of local agriculture in Sukawangi Village, Sumedang Regency, West Java. In this paper, the authors describe the solution proposals related to the problems of coffee farmers in Sukawangi, Sumedang. Two suggestions for increasing the value added of coffee beans are by using appropriate technology adoption. With both of these solutions, it is expected that the quality of coffee plantation in Sumedang can be increased and can be marketed in both domestic and international markets. As a result, the welfare of farmers in Sumedang will increase.

Phylogenetic analysis of 23 accessions of Indonesian banana cultivars based on Internal Transcribed Spacer 2 (ITS2) region Karlia Meitha; Intan Fatmawati; Fenny Martha Dwivany; Agus Sutanto; Sigit Nur Pratama; Husna Nugrahapraja; Ketut Wikantika
Indonesian Journal of Biotechnology Vol 25, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.49506

Pisang Kepok (Musa spp. [ABB ’Saba’ subgroup]) has several unique characteristics, such as tolerance to drought and Fusarium Foc (TR4) disease. Currently, the genetic diversity of Pisang Kepok in Indonesia is not well identified, although it is widely cultivated. Information on genetic diversity is essential for developing breeding strategies to achieve efficient cultivar improvement in the future. Aims of this research were to analyze the genetic variation of Pisang Kepok from some islands in Indonesia and to determine the genetic relationship between Pisang Kepok and other accessions banana cultivars based on ITS2 region, as a basis for future research in improving banana quality through molecular breeding. We have conducted the multiple sequence alignment and built the phylogenetic tree analysis using the Bayesian Inference Phylogeny method of one million generations (ngen = 1,000,000). The ITS2 region showed two clade ingroups: first clade consists of banana with B genome (balbisiana), while the second clade consists of banana with only A genome (acuminata). In general, all accessions of Pisang Kepok cultivars were clustered in the B genome of bananas cultivars. In addition, the ITS2 sequences and secondary structures among Pisang Kepok from various regions are identical, suggesting that there was no genetic variation in the ITS2 region of Pisang Kepok from multiple areas in Indonesia.

Expression profiling of the CHS8, CHI1A, IFS2, and CHR genes in black soybean seed [Glycine max (L). Merr.] of F4 generation Dadang Sumardi; Aulia Marwah Mumtaza; Rijanti Rahaju Maulani; Adi Pancoro; Husna Nugrahapraja; Sony Suhandono; Tati Suryati Syamsudin; Agung Kurniawan
Indonesian Journal of Biotechnology Vol 24, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.51888

Black soybean [Glycine max (L.) Merr.] produces isoflavones as secondary metabolites, which have many benefits for human health and plant defense system. Expression profiling can guide potential work in functional genomics of the isoflavone biosynthesis pathway. Previous studies showed the vital role of the CHS8, CHI1A, and IFS2 genes in isoflavone biosynthesis. However, expression profiling of these genes in the local black soybean varieties is still limited. This study investigated the gene expression levels of the CHS8, CHI1A, IFS2, and CHR genes in local varieties, namely, UP106 (high isoflavone) and UP122 (low isoflavone) and its progenies, i.e., UP106xUP122 and UP122xUP106. Relative gene expression profiling was conducted on the basis of Reverse Transcriptase Polymerase Chain Reaction (RT‐PCR) with ACT2/7 as a housekeeping gene. As a result, the expression level of CHS8 in UP122 is lower than that in UP106. No significant difference in the expression level of CHI1A was observed in all samples. The expression levels of CHS8 and CHI1A in both progenies were higher than that in the parental line, whereas the expression levels of IFS2 in both progenies were lower than that in the parental line. CHS8 and IFS2 expression from UP106xUP122 was higher than that from UP122xUP106, whereas CHI1A expression from UP122xUP106 was higher than that from UP106xUP122. CHR showed a high expression in the reciprocal cross; however, this expression did not exceed from UP106. In conclusion, the crossing between parental lines did not affect the gene expression level in the isoflavone biosynthesis pathway.

Revisiting pollen-pistil interaction and cross incompatibility in maize Husna Nugrahapraja; Edoardo Bertolini; Mario Enrico Pe
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2019.1.1/DTCS2650

The review addressed aspects of plant fertilisation and the phenomenon of genetic cross-incompatibility in maize controlled by the Gametophyte1 locus. This phenomenon determines the failure to accomplish successful fertilisation and a full seed set when pollen grains carrying the ga1 allele pollinate female inflorescences carrying the Ga1-strong (Ga-1s) allele in the homozygous state (Ga1-s/Ga1-s). We divided the review work into several topics — first, the introduction of sexual plant reproduction. Second, pollen-pistil interactions in plants. Third, reproductive barriers during plant reproduction. Third, Incompatibility in plants. Fourth, fine mapping of the Ga1 locus in maize. Fifth, recent researches on Ga1-related cross-incompatibility in maize.

MINI REVIEW: PENGEMBANGAN BIOSENSOR KOLORIMETRI BERBASIS AGREGASI NANOPARTIKEL EMAS Hana Verdiani; Dinda Syafira; Husna Nugrahapraja
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.14657

Gold nanoparticle (AuNP) is a material commonly used in colorimetric detection for biological sensors due to its low production cost, simplicity, and ease use. AuNP is known to generate different colors depending on the particle aggregation level. In the last two decades, changes in AuNP aggregation status have become the basis for developing a sensor to detect analytes in the sample. The solution color changes could be observed without sophisticated equipment, allowing ,more straightforward interpretation of the detection result. This review focuses on developing AuNP aggregation-based colorimetric tests for detecting various biological analytes. It discusses multiple mechanisms, strategies, functionalization, the latest research, and perspectives of AuNP use in colorimetric detection, which could be considered for future biosensor development. The concept of colloidal gold nanoparticle aggregation and stabilization is emphasized in this review.

MINI REVIEW: PENGEMBANGAN BIOSENSOR KOLORIMETRI BERBASIS AGREGASI NANOPARTIKEL EMAS Hana Verdiani; Dinda Syafira; Husna Nugrahapraja
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.14657

Gold nanoparticle (AuNP) is a material commonly used in colorimetric detection for biological sensors due to its low production cost, simplicity, and ease use. AuNP is known to generate different colors depending on the particle aggregation level. In the last two decades, changes in AuNP aggregation status have become the basis for developing a sensor to detect analytes in the sample. The solution color changes could be observed without sophisticated equipment, allowing ,more straightforward interpretation of the detection result. This review focuses on developing AuNP aggregation-based colorimetric tests for detecting various biological analytes. It discusses multiple mechanisms, strategies, functionalization, the latest research, and perspectives of AuNP use in colorimetric detection, which could be considered for future biosensor development. The concept of colloidal gold nanoparticle aggregation and stabilization is emphasized in this review.Â

Development Of Appropriate Technology Adoption For Coffee Farmers In Sukawangi Village, Sumedang Bentang Arief Budiman; Hari Purnama; Husna Nugrahapraja; Muhammad Abdur Rasyid; Natas Setiabudhi Daryono Putra; Sri Aswin Suryani; Annisa Ratna Nurillah; Ima Mulyama Zainuddin; Rindia Maharani Putri; Wervyan Shalannanda; Raden Aswin Rahadi
Jurnal Ilmu Sosial Politik dan Humaniora (JISORA) Vol 2 No 2 (2019): Jurnal Ilmu Sosial Politik dan Humaniora
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik - Universitas Garut

In this paper, the authors propose solutions to increase the added value of coffee in the form of the process of making the process of making coffee using appropriate technology adoption. This paper uses qualitative methods of locational survey and literature study. Survey activities are conducted in the form of interviews and observations. The team conducted interviews with coffee farming communities in the area and observed the conditions of coffee farming. The literature study was conducted by synthesizing literature reviews and continued by formulating the theory and principles of appropriate technology in the context of local agriculture in Sukawangi Village, Sumedang Regency, West Java. In this paper, the authors describe the solution proposals related to the problems of coffee farmers in Sukawangi, Sumedang. Two suggestions for increasing the value added of coffee beans are by using appropriate technology adoption. With both of these solutions, it is expected that the quality of coffee plantation in Sumedang can be increased and can be marketed in both domestic and international markets. As a result, the welfare of farmers in Sumedang will increase.

Diversity analysis of moray eel (muraenidae) on artificial reef structure in mengiat beach, Nusa Dua, Bali Nathanael Wilbert; Husna Nugrahapraja
Environmental and Materials Vol. 1 No. 1: Juni (2023)
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/eam.v1i1.2023.62

Optimal coral growth has a positive impact on increasing biodiversity. This can be observed through the increase in the number of marine species as the transplanted coral reefs age. One natural indicator in coastal ecosystems is the presence of moray eels. Within the coral reef ecosystem, the diversity of moray eels as natural predators belonging to the category of reef fish can serve as an indication of a healthy and balanced ecosystem. The method used in this research is visual counting or visual observation of several species of marine eels on three different major installed reef star media, as part of coral reef restoration efforts. The data collection locations include three points of reef star media installation: BTN, Coral Garden, and Cluster 5. The water conditions in these locations, such as salinity, temperature, pH, and conductivity, indicate the adequate quality to support coral reef restoration efforts. In this study, five species of marine eels frequently encountered in the three data collection locations identified as: Echidna nebulosa, Gymnothorax thyrsoideus, Gymnothorax fimbriatus, Gymnothorax pictus, and Gymnothorax richardsonii. The highest abundance and diversity were found in Coral Garden, followed by Cluster 5, with BTN being the lowest.

Study of the sea urchins (echinoidea) influence on the coral reef communities in the Nusa Dua Bali conservation area Vonny Angellia; Husna Nugrahapraja
Environmental and Materials Vol. 1 No. 1: Juni (2023)
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/eam.v1i1.2023.63

Coral reef ecosystems are crucial for marine ecosystems as they support various other marine organisms. Sea urchins, such as Diadema setosum and Echinometra mathaei influence the coral reef communities by consuming algae that disrupt coral growth. This study aims to determine reef star coral media quantity, identify the dominant coral species at three points (BTN, Coral Garden, and Cluster 5) in Nusa Dua Beach, and assess the influence of sea urchins on coral reefs. The sampling method used was Purposive sampling, and visual observations which were conducted from June 27 to June 30, 2022. Results showed the varying sea urchin populations, with Acropora sp. and Pocillopora sp. dominating at BTN, Acropora sp. and Pectinia sp. at Coral Garden, and Acropora sp. at Cluster 5. The findings suggest that sea urchins play a key role in maintaining coral reefs by consuming macroalgae and supporting coral survival.

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