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All Journal El-Hayah : Jurnal Biologi Biosaintifika: Journal of Biology & Biology Education Jurnal Labora Medika
Arya Iswara
Jurusan Biologi Fakultas MIPA Universitas Negeri Semarang Jalan Raya Sekaran Gunungpati Semarang 50229
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology

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Efek Pemberian Antioksidan Vitamin C dan E terhadap Kualitas Spermatozoa Tikus Putih Terpapar Allethrin Christijanti, Wulan; Utami, Nur Rahayu; Iswara, Arya
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1148

Abstract

Allethrin  is  one  of  synthetic  pyrethroid types which  is  used  in many mosquito repellents and has toxic potential. The aim of this research was to examine the infl uence of vitamin C and E antioxidant activity to the sperm quality of allethrin-induced albino rats. This research used a post-test randomized design. The population of the research were  8  hr/day  allethrin-induced male  albino  rats. The  sample  of  the  research was allethrin-induced male albino rats which were divided into 4 groups, i.e. group I was the control group, group II was the group treated with 1.8 mg vitamin C per day, group II was the group treated with 1.44 mg vitamin E per day, and group IV was treated with a combination of 1.8 mg vitamin C per day and 1.44 mg vitamin E per day. The duration of the treatment was 45 days. The sperm sample was taken in day 46. The data consisted of sperm count or amount, sperm motility, sperm viability, and sperm morphology, and then was analyzed with ANOVA followed by BNT. The result showed that vitamin C and E antioxidants signifi cantly infl uenced the the sperm quality of the 45-day mosquito repellent-induced rats. Overall, the allethrin-induced albino rats treated with vitamin C and E antioxidants were better in maintaining the sperm quality than single treatment of vitamin antioxidants.Keywords: allethrin, vitamin C, vitamin E, antioxidant, sperm  quality.
Pengaruh Variasi Waktu Clearing Terhadap Kualitas Sediaan Awetan Permanen Ctenocephalides Felis Iswara, Arya
Jurnal Labora Medika Vol 1, No 1 (2017): Jurnal Labora Medika
Publisher : Jurnal Labora Medika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sediaan awetan permanen adalah teknik pengawetan preparat untuk berbagai macam parasit, salah satunya adalah Ctenocephalides felis. Proses pembuatan preparat awetan melalui tahapan clearing. Clearing merupakan proses perendaman didalam larutan xylol selama 15 menit yang bertujuan menjadikan struktur Ctenocephalides felis terlihat jernih. Hasil penjernihan akan menghasilkan hasil yang maksimal apabila digunakan waktu perendaman dalam xylol selama semalam. Hasil perendaman semalam dapat memperlihatkan struktur tubuh Ctenocephalides felis yang lebih jelas, jernih dan transparan. Lamanya waktu penjernihan yang diperlukan dirasa kurang efektif sehingga dirasa untuk dilakukan penelitian penggunakan waktu yang lebih pendek, penelitian ini bertujuan untuk mengetahui pengaruh variasi waktu clearing terhadap kualitas sediaan awetan permanen Ctenocephalides felis. Penelitian ini merupakan penelitian eksperimen. Sampel yang didapatkan diproses untuk dilakukan pembuatan sediaan permanen dengan menggunakan 3 variasi waktu clearing yaitu 5, 15 dan 25 menit.Hasil penelitian menunjukkan kualitas sediaan dengan perlakuan clearing 25 menit lebih baik dibandingkan kualitas sedian dengan perlakuan clearing 15 menit dan 5 menit. Penelitian ini membuktikan bahwa semakin lama dilakukan proses clearing maka semakin baik kualitas sediaan awetan permanen yang didapatkan.
Antibacterial Activity of Combination Cold Plasma and Parijoto (Medinilla speciosa) Against Staphylococcus aureus and Pseudomonas aeruginosa on Diabetic Ulcer Anastasia, Fajar Farra; Aziz, Itsna Risqii; Oktaviola, Vanny; Iswara, Arya
El-Hayah : Jurnal Biologi Vol 7, No 4 (2020): EL-HAYAH (VOL 7, NO 4 MARET 2020)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i4.10313

Abstract

This study aims to determine the potential of cold plasma (cold plasma) as an antibacterial if combined with Parijoto (Medinilla speciosa) extract against the dominant bacteria in diabetic ulcer infection, namely Gram-positive Staphylococcus aureus and Gram-negative bacteria Pseudomonas aeruginosa with a concentration of 25%, 50 %, 75%, and 100%. This study was an experimental test in vitro using the well diffusion method. The well diffusion method uses an MHA given a 5mm diameter and inserted a 100uL sample then incubates 35 ± 2 ° C for 16-18 hours. The results showed antibacterial activity from Cold Plasma with a 10 mm shot distance for 3 minutes with inhibitory zones against S. Aureus 3 mm and against P. aeruginosa 3.35 mm, a combination of Cold Plasma antibacterial activity with parijoto extract with a zone of inhibition of S. aureus 11.7 mm (25%), 14.1 (50%), 16.5 (75%), 17.1 (100%) and against P. aeruginosa 7.7 mm (25%), 9.3 mm (50%), 10.1 mm (75%), 11.7 (100%). the results of the Cold Plasma inhibition zone and Cold Plasma combination with parijoto extract 100% concentration of S. aureus were greater than the inhibition zone for P. aeruginosa. Cold Plasma combination inhibition zones with starch extracts against S. aureus compared to Vancomicin (VA) antibiotics with a 19.9 mm inhibition zone included in the intermediate category, Cold Plasma combination inhibition zone with parijoto juice against P. aeruginosa bacteria compared with Meropenem antibiotics ( MRP) with a 9.7mm inhibition zone included in the sensitive category
Antimicrobial Activity and Identification The Compounds of Methanol Extract from The Pleurotus Ostreatus Fruiting Body Prastiyanto, Muhammad Evy; darmwati, Sri; iswara, Arya; Setyaningtyas, Agnis; Trisnawati, Laily; Syafira, Anna
El-Hayah : Jurnal Biologi Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i1.4082

Abstract

Pleurotus ostreatus is an edible mushroom that also has potential as medicinal values. In this study, fruiting body of P. ostreatus was tested for its ability to inhibit the growth of fungi and bacteria. The fruiting body powder of P. ostreatus was extracted using methanol by maceration method. Analysis of this compound was done by using anisaldehid sulfuric acid, Dragendorff reagent, and FeCl3. Using the agar well diffusion technique, the extracts were tested for their antimicrobial activity against Staphylococcus aureus (Gram positive), Enterobacter aerogenes (Gram negative) and Candida albican (yeast). The spot results on TLC using crude extract of P. ostreatus is terpenoids. Zone of inhibition for the various extracts varied between 10.9 - 23.2 mm. Ten miligrams extract exhibit maximum antimicrobial activity against most of the tested pathogens 
Bacterial Plasmids Profile from Escherichia coli Resistant to Metronidazole and Nalidixic Acid Iswara, Arya; Dewi, Sri Sinto
El-Hayah : Jurnal Biologi Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i1.4079

Abstract

bacteria that cause an illness. Antibiotic treatments to a patient have a purpose to eliminate the pathogen bacteria. Bacteria resistance to antibiotic was influenced by the intensity of antibiotic treatment in a region, the uncontrolled antibiotics treatments would increase the antibiotic resistance of bacteria. Plasmids was an extrachromosomal DNA that encodes a functional protein that would eliminate the antibiotic activity. Plasmid is the determinant of bacteria sensitivity to antibiotics. In this case it would be important to find out the bacterial plasmid profile on the E.coli resistant to metronidazole and nalidixic acid antibiotics. This research was using four different sample from faces of diarrhea, ice block, waters from well, and ketchup to cultivate the E. coli. lasmid isolation method was carried out by lyses alkali method. Plasmid profile of the E. coli that resistant to metronidazole and nalidixic acid antibiotics and analyzed using electrophoresis on 1% agarose. E. coli plasmid DNA profile was observed as a fluorescent DNA band in ultraviolet rays. In result, isolated plasmids from bacteria that resistant to antibiotics metronidazole and nalidixic acid having similar size approximately 500 bp, different from bacteria that sensitive to antibiotics metronidazole and nalidixic acid has a smaller size in region of 100 bp.
Co-Authors Anastasia, Fajar Farra Aziz, Itsna Risqii darmwati, Sri Nur Rahayu Utami Oktaviola, Vanny Prastiyanto, Muhammad Evy Setyaningtyas, Agnis Sri Sinto Dewi Syafira, Anna Trisnawati, Laily Wulan Christijanti