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All Journal El-Hayah : Jurnal Biologi Biosaintifika: Journal of Biology & Biology Education Jurnal Labora Medika
Arya Iswara
Jurusan Biologi Fakultas MIPA Universitas Negeri Semarang Jalan Raya Sekaran Gunungpati Semarang 50229
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology

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Journal : El-Hayah : Jurnal Biologi

 1 
Antibacterial Activity of Combination Cold Plasma and Parijoto (Medinilla speciosa) Against Staphylococcus aureus and Pseudomonas aeruginosa on Diabetic Ulcer Anastasia, Fajar Farra; Aziz, Itsna Risqii; Oktaviola, Vanny; Iswara, Arya
El-Hayah : Jurnal Biologi Vol 7, No 4 (2020): EL-HAYAH (VOL 7, NO 4 MARET 2020)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i4.10313

Abstract

This study aims to determine the potential of cold plasma (cold plasma) as an antibacterial if combined with Parijoto (Medinilla speciosa) extract against the dominant bacteria in diabetic ulcer infection, namely Gram-positive Staphylococcus aureus and Gram-negative bacteria Pseudomonas aeruginosa with a concentration of 25%, 50 %, 75%, and 100%. This study was an experimental test in vitro using the well diffusion method. The well diffusion method uses an MHA given a 5mm diameter and inserted a 100uL sample then incubates 35 ± 2 ° C for 16-18 hours. The results showed antibacterial activity from Cold Plasma with a 10 mm shot distance for 3 minutes with inhibitory zones against S. Aureus 3 mm and against P. aeruginosa 3.35 mm, a combination of Cold Plasma antibacterial activity with parijoto extract with a zone of inhibition of S. aureus 11.7 mm (25%), 14.1 (50%), 16.5 (75%), 17.1 (100%) and against P. aeruginosa 7.7 mm (25%), 9.3 mm (50%), 10.1 mm (75%), 11.7 (100%). the results of the Cold Plasma inhibition zone and Cold Plasma combination with parijoto extract 100% concentration of S. aureus were greater than the inhibition zone for P. aeruginosa. Cold Plasma combination inhibition zones with starch extracts against S. aureus compared to Vancomicin (VA) antibiotics with a 19.9 mm inhibition zone included in the intermediate category, Cold Plasma combination inhibition zone with parijoto juice against P. aeruginosa bacteria compared with Meropenem antibiotics ( MRP) with a 9.7mm inhibition zone included in the sensitive category
Antimicrobial Activity and Identification The Compounds of Methanol Extract from The Pleurotus Ostreatus Fruiting Body Prastiyanto, Muhammad Evy; darmwati, Sri; iswara, Arya; Setyaningtyas, Agnis; Trisnawati, Laily; Syafira, Anna
El-Hayah : Jurnal Biologi Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i1.4082

Abstract

Pleurotus ostreatus is an edible mushroom that also has potential as medicinal values. In this study, fruiting body of P. ostreatus was tested for its ability to inhibit the growth of fungi and bacteria. The fruiting body powder of P. ostreatus was extracted using methanol by maceration method. Analysis of this compound was done by using anisaldehid sulfuric acid, Dragendorff reagent, and FeCl3. Using the agar well diffusion technique, the extracts were tested for their antimicrobial activity against Staphylococcus aureus (Gram positive), Enterobacter aerogenes (Gram negative) and Candida albican (yeast). The spot results on TLC using crude extract of P. ostreatus is terpenoids. Zone of inhibition for the various extracts varied between 10.9 - 23.2 mm. Ten miligrams extract exhibit maximum antimicrobial activity against most of the tested pathogens 
Bacterial Plasmids Profile from Escherichia coli Resistant to Metronidazole and Nalidixic Acid Iswara, Arya; Dewi, Sri Sinto
El-Hayah : Jurnal Biologi Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i1.4079

Abstract

bacteria that cause an illness. Antibiotic treatments to a patient have a purpose to eliminate the pathogen bacteria. Bacteria resistance to antibiotic was influenced by the intensity of antibiotic treatment in a region, the uncontrolled antibiotics treatments would increase the antibiotic resistance of bacteria. Plasmids was an extrachromosomal DNA that encodes a functional protein that would eliminate the antibiotic activity. Plasmid is the determinant of bacteria sensitivity to antibiotics. In this case it would be important to find out the bacterial plasmid profile on the E.coli resistant to metronidazole and nalidixic acid antibiotics. This research was using four different sample from faces of diarrhea, ice block, waters from well, and ketchup to cultivate the E. coli. lasmid isolation method was carried out by lyses alkali method. Plasmid profile of the E. coli that resistant to metronidazole and nalidixic acid antibiotics and analyzed using electrophoresis on 1% agarose. E. coli plasmid DNA profile was observed as a fluorescent DNA band in ultraviolet rays. In result, isolated plasmids from bacteria that resistant to antibiotics metronidazole and nalidixic acid having similar size approximately 500 bp, different from bacteria that sensitive to antibiotics metronidazole and nalidixic acid has a smaller size in region of 100 bp.
Co-Authors Anastasia, Fajar Farra Aziz, Itsna Risqii darmwati, Sri Nur Rahayu Utami Oktaviola, Vanny Prastiyanto, Muhammad Evy Setyaningtyas, Agnis Sri Sinto Dewi Syafira, Anna Trisnawati, Laily Wulan Christijanti