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All Journal International Journal of Evaluation and Research in Education (IJERE) Cakrawala Pendidikan Jurnal Penelitian Saintek Jurnal Pendidikan Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA) Agroista: Jurnal Agroteknologi Jurnal Edukasi Biologi
Suyitno Aloysius, Suyitno
Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Materials Science & Nanotechnology Physics Social Sciences

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Journal : Agroista: Jurnal Agroteknologi

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Optimasi Induksi Tunas Aksiler Dendrobium nobile Melalui Kombinasi 2-iP dan 2,4-D In Vitro Sofiatul Milah; Lili Sugiyarto; Ratnawati; Suyitno Aloysius; Ixora Sartika Mercuriani
AGROISTA : Jurnal Agroteknologi Vol. 7 No. 1 (2023): MEI
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55180/agi.v7i1.613

Abstract

This research aims to determine optimum combination of addition 2-iP and 2,4-D with different concentration and the nodus position for induction Dendrobium Red Emperor ‘Prince’ axial shoots. The research employed a CFR design with two factors, namely: variation of combined concentration 2-iP and 2,4-D (A: 1 ppm 2-iP + 2 ppm 2,4-D; B: 2 ppm 2-iP + 1,5 ppm 2,4-D; C: 3 ppm 2-iP + 1 ppm 2,4-D; D: 4 ppm 2-iP + 0,5 ppm 2,4-D) each treatment was repeated 6 times and the nodal position (top, middle, bottom). The basic medium is NP+CW. The axillary bud growth is measured based on the time of bud emerge, shoot growth, explant percentage which form axillary bud, root growth, and plantlet wet weight. Data obtained were analyzed using ANOVA and continued with DMRT 5%. Results show that B treatment give fastest bud emerging time, and highest stem diameter, buds height, leaves length, leaves width, roots length. A treatment shows best results on shoots number, plantlet wet weight, number of leaves, and number of roots. The optimum combined concentration of 2-iP and 2,4-D to induce axillary shoots is 2 ppm 2-iP + 1,5 ppm 2,4-D, whilst best nodus position is the top one.
Co-Authors Ayu Candra Juniarti Ayu Purnamasari Heru Kuswanto Hidayah Ina Qodriyani Ixora Sartika Mercuriani Lili Sugiyarto Lili Sugiyarto Lili Sugiyarto Nur Aeni M.P. Ariyanti Paidi, Paidi Putri Anjani Nawang Bulan Qodriyani, Hidayah Ina Ratna Dyah Hartanti Ratnawati Ratnawati Ratnawati Rifqi Rasis Rizki Khairani Shesilya Slamet Suyanto Sofiatul Milah Suhartini Tasya Novian Indah Sari