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Antagonis Pseudomonas fluorescens indegenous terhadap Ralstonia solanacearum pada Tanaman Tomat (Lycopersicum esculentum) Armaleni Armaleni; Nasril Nasir; Anthoni Agustien
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p19

Abstract

Tomato (L. esculentum) is one of the most important vegetable crops produce in West Sumatra. In this province, tomato are cultivated at highland regions of Solok, Padang Panjang, Tanah Datar and Bukittinggi. Annually, the increasing demand of this crop comes from other provinces in Sumatra and Java. This opportunity stimulate tomato’s agribusiness activity in West Sumatra. However, the crop succumb to the most destroyed bacterial wilt disease caused by Ralstonia solanacearum. The aims of this study to select antagonist Pseudomonas fluorescent as a biological control agent against R.solanacearum. The candidates of antagonist P. fluorescetn were collected from diseased tomato with R. solanacearum’s symptom in several tomato’s plantations in West Sumatra. Study of the antagonist was conducted from March to July 2016 at the Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences Andalas University. To find the antagonist work, each of the collected candidate was grown opposite R. solanacearum on Nutrient Agar medium. The result found that there were16 isolates of P. fluorescent occurred. The best antagonist ability amongst the candidates was from Pfi with diameter zone was 4,95 cm.Keywords: tomato, Ralstonia solanacearum, Pseudomonas fluorescens, biological control agent, Pfi