I Gede Krisna Putra Pratama
JURUSAN TEKNOLOGI INDUSTRI PERTANIAN FAKULTAS TEKNOLOGI PERTANIAN UNIVERSITAS UDAYANA

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AKTIVITAS BIODESULFURISASI DIBENZOTIOFENA DALAM MODEL MINYAK TETRADEKANA PADA RASIO MINYAK AIR DAN KONSENTRASI RESTING SEL ISOLAT BAKTERI SBJ8 I Gede Krisna Putra Pratama; Ida Bagus Wayan Gunam; G.P. Ganda Putra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 5 No 1 (2017): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

This study aims to determine the best ratio of oil / water and concentration of resting cells and determine the time course of resting cells bacteria isolates SBJ8 to be known the biodesulfurization capability in two-phase media using media mineral salt sulfur free-tetradecane(MSSF-TD). Oil/water ratio used in this study is 1 : 1 , 1 : 2 and 1 : 4 in combination with cell concentration by OD66010, 20, and 30. The study was conducted using 200 mg · l–1dibenzothiophene as a source of sulfur dissolved in tetradecane as a model of petroleum (oil phase). Isolates were incubated for 24 hours at 37°C and the residue analyzed by gass cromathography mass selective (GC-MS). Testing the degradation of resting cells is accomplished by two replications. The results showed that the ratio of oil water 1 : 4 by and cellconcentration OD66030 shows the results of the highest degradation of 84.84% and the time course of 24 hours resting cells show degradation rate dibenzothiophene up to 81.84%. Keywords : Biodesulfurization, dibenzothiophene, tetradecane, resting cells