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Gede Suastika
Departemen Proteksi Tanaman, Fakultas Pertanian Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry

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PEMANFAATAN BAKTERI PELARUT FOSFAT PENGINDUKSI HORMON IAA (Indol Acetic Acid) UNTUK PENINGKATAN PERTUMBUHAN KEDELAI ( Glycine max) Desak Putu Sudarmini; I Made Sudana; I Putu Sudiarta; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Soybean is one of the agricultural commodities that are needed to fulfil food nutrients of Indonesian people. The low production of this commodity in Indonesia has not yet been able to meet the high demand for soybean that reaches 2.8 million tons per year, so that the Indonesian government still import soybeans at the amount of 2.26 million tons annually. The low production of soybeans in Indonesia is due to several factors, one of which is the expensive price of chemical fertilizers The use of rhizobacteria as bio-controlling agents can increase the availability of nutrients as well as crop production. Phosphate solubilizing bacteria are rhizobacteria that have the capacity to solubilize bound phosphate as well as trigger the growth hormone IAA that is the main hormone that controls various physiological processes in plants. The current study aimed to investigate whether isolates derived from rhizospheres of Leguminosae plants can induce IAA hormone and foster the vegetative growth of soybeans.The research results showed that five out of nine isolates of the phosphate solubilizing bacteria are able to induce IAA hormone and foster the vegetative growth of soybeans. Isolate treatment that showed the best impact toward soybean growth was identified molecularly through PCR. The result of RHC6 isolate identification shows a DNA ladder size of 1300bp which is suitable with the primer used. Based on the phylogenetic analysis conducted, nucleotide sequence of RHC6 isolate from Bali has a high similarity (97%) to and a low genetic distance (0.018) from Brevundimonas diminuta isolate from the US.
BAKTERI PELARUT FOSFAT MEMICU KETAHANAN TANAMAN KEDELAI (Glycine max) TERHADAP PENYAKIT SOYBEAN MOSAIC VIRUS Ni Nyoman Alit Purwaningsih; I Dewa Nyoman Nyana; I Gede Rai Maya Temaja; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.983 KB)

Abstract

Soybean (Glycine max) is an important commodity in Indonesia. The high utilization of soybean has not been accompanied by the sufficiency of amount of its production, even decreased from year to year. One of the factors that cause the decreased of soybean productivity is soybean mosaic disease caused by viral pathogens. Soybean mosaic virus is one of the most important types of virus in soybean could cause a decreased in yield until 25-90%.This study aimed to test rhizobacteria isolated from the rhizosphere of Leguminosae plants that able to providing resistance to SMV disease on soybean plant. Six isolates rhizobacteria that could be isolated from Leguminosae plants had used as a treatment in the test. The result of field test showed three isolates namely R13, R12, and R9 able to increase soybean plant resistance, so each isolates made influence to crop yield. The results of identification based on the analysis of 16S rRNA gene, isolate R13 which gave the best response to soybean plant resistance and had percentage similarity between 94-98% with species Empedobacter brevis (Flavobacterium breve).
Co-Authors Desak Putu Sudarmini I Dewa Nyoman Nyana I G. R. M. TEMAJA I Made Sudana Ni Nyoman Alit Purwaningsih Putu Sudiarta