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All Journal Jurnal Fitopatologi Indonesia IPTEKMA SOCA: Jurnal Sosial Ekonomi Pertanian AGRITROP International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology Bumi Lestari E-Jurnal Manajemen Universitas Udayana Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Ilmu Lingkungan Cropsaver : Journal of Plant Protection

I G. R. M. TEMAJA

Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana

Author-ID : 2452906

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Medicine & Pharmacology

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PEMBERIAN EKSTRAKURIKULER PERTANIAN PADA SISWA SD DI DESA GUNAKSA KABUPATEN KLUNGKUNG, BALI Rahmawatiningsih, Ayu; Prayoga, I Made Angga; Yanthi, Ni Putu Widyami; Temaja, I Gede Rai Maya
IPTEKMA Volume 2 No.1 - Desember 2010
Publisher : Bidang Kemahasiswaan Universitas Udayana

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Kata-kata Henry Kissinger,“Control oil and you can control the nations, control food and you control the people”, menjelaskan bahwa makanan berperan sangat penting dalam kehidupan manusia. Pertanian memiliki peran yang sangat penting bagi manusia. Namun sektor pertanian di Indonesia mulai terpinggirkan. Salah satunya dengan minimnya pemuda yang menggeluti bidang ini. Jika hal ini dibiarkan terus dikhawatirkan kelak pertanian akan kekurangan tenga kerja yang berkualitas. Melihat hal tersebut, perlu pengenalan pertanian kepada generasi muda sejak dini agar tumbuh minat generasi muda menekuni pertanian kelak. Salah satunya melalui pemberian ekstrakurikuler pertanian pada siswa SD di Desa Gunaksa, kabupaten Klungkung. Tujuan dari ekstrakurikuler ini adalah mengenalkan dan menjelaskan tentang peran penting pertanian, menjelaskan tentang potensi pertanian dan mempraktekkan langsung metode pertanian yang memungkinkan dilakukan oleh siswa SD. Pengenalan pertanian diberikan dalam bentuk ekstrakurikuler karena selama ini pertanian tidak diajarkan dan dipraktekkan di SD. Materi yang akan disampaikan seputar teknologi pertanian yang sederhana seperti perbanyakan tanaman, pembuatan pupuk, teknik menanam serta penjelasan-penjelasan ilmiah mengenai proses tumbuh suatu tanaman. Hasil dari kegiatan ekstrakurikuler ini siswa SD dapat mengetahui dan mempraktekkan cara menanam yang tepat. Hal ini dapat dilihat dari meningkatnya jumlah tanaman yang hidup ditiap minggunya serta terjadinya peningkatan minat siswa terhadap pertanian. Selain itu, terjadi peningkatan persentase kesukaan siswa terhadap pertanian sebanyak 16% serta terjadi peningkatan persentase keinginan be kerja di bidang pertanian sebanyak 29%.

KARAKTERISASI CHRYSANTHEMUM STUNT VIROID (CSVD) YANG MENGINFEKSI KRISAN DI INDONESIA (CHRYSANTHEMUM STUNT VIROID (CSVD) CHARACTERIZE INVECTED CHRYSANTHEMUM IN INDONESIA) TEMAJA, I G.R.M.
AGRITROP Vol. 29, No. 1 Maret 2010
Publisher : AGRITROP

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Chrysanthemum stunt viroid (CSVd) is an important disease of the florist’s chrysanthemum caused by viroid. The symptoms of CSVd disease were variable and are highly depend on both of cultivar and environmental conditions. The main symptom is stunting, causing the reduction of weight in overall of mature plants 30 to 50%. The other common symptoms in floral showed the infected plants having reduction of flower size and demonstrating premature flowering. CSVd is mainly transmitted by propagation, when cuttings have been taken from infected mother plants. Our survey on growing fields in Baturiti (Bali), Cipanas and Bandung (West Java), and Medan (North Sumatera) found 51.22% of samples to be infected by CSVd based on the bioassay using S. cruentus. The infections of CSVd were found in all of survey location. Using the specific primers for CSVd, a 250 bp DNA product was successfully amplified by RT-PCR technique. By sequencing analysis, Indonesian isolates were classified into two groups. The first group consist of the isolates from Cipanas, Medan and Bandung, and another group was Baturiti isolate.

Deteksi Chrysanthemum B Carlavirus (CVB) pada Tanaman Krisan di Indonesia TEMAJA, I G. R. M.; SUASTIKA, G.; HIDAYAT, SH.; KARTOSUWONDO, U.
AGRITROP Vol. 26, No. 1 Maret 2007
Publisher : AGRITROP

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In a survey of chrysanthemum growing fields and greenhouses in Brastagi, Sumatera Utara; Cianjur, JawaBarat; Malang, Jawa Timur; and Tabanan and Buleleng, Bali, some chrysanthemum cultivars were found showingmild leaf mottling, vein-clearing or vein banding of leaves and slight loss of flower quality. Out of 287 samplessurveyed, the disease incidence ranged between 7.50% and 67.44%. Through DAS-ELISA, the saps extractedfrom diseased plant samples, 34.84% were found to be positively reacted with serum anti-CVB, but not with seraanti- CMV, -TSWV, -PVY and -TMV. The virus isolate was then designed as CVB Indonesian isolate. The viruswas sap-transmissible to narrow host range. The virus induced systemic symptom on Nicotiana benthamiana, N.clevelandii, N. tabacum var. Burley, N. tabacum var. Havana; local necrotic lesion on Chenopodium amaranticolo,C. quinoa and both local and systemic symptom on Petunia hybrida.

Penyakit Layu Bakteri Stewart pada Jagung di Bali I Gede Rai Maya Temaja; G.N. Alit Susanta Wirya; Ni Made Puspawati; Khairun Nisak Syahdu
Jurnal Fitopatologi Indonesia Vol. 13 No. 5 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Stewart’s wilt is a serious disease of sweet corn (Zea mays). The typical symptoms of the disease are pale-green to yellow linear streaks parallel to the veins. The symptoms were observed on sweet corn in Denpasar, Tabanan, Gianyar, and Karangasem areas during a survey in 2015. Pathogen detection based on a polymerase chain reaction was carried out using total DNA obtained from symptomatic leaf samples and the pairs of primers, CPSL1/CPSR2c. The expected sized (~1100 bp) amplicon was detected in samples from Denpasar. Sequence analysis confirmed that Stewart’s wilt disease symptoms are caused by Pantoea stewartii subsp. stewartii. Nucleotide sequence and phylogenetic analysis showed that P. stewartii subsp. stewartii from Bali has high homology (98.97-99.08 %) and placed in the same clade with isolates from Canada, USA and Japan. This is the first report of P. stewarti subsp. stewartii on corn in Bali.

Kloning Gen Transcriptional Activator Protein dan Replication-Associated Protein dari Squash leaf curl China virus Penyebab Penyakit Daun Kuning pada Tanaman Mentimun Ni Nengah Putri Adnyani; I Dewa Made Putra Wiratama; I Gede Rai Maya Temaja; Sari Nurulita; Gede Suastika
Jurnal Fitopatologi Indonesia Vol. 14 No. 1 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow disease on Cucurbitaceae caused by Squash leaf curl China virus (SLCCNV; Begomovirus) is an important disease in many countries. Typical yellow leaf curl symptoms was found on cucumber in Tabanan, Bali. The objective of this research is to clone Transcriptional Activator Protein (TrAp) and Replication-Associated Protein (Rep) of SLCCNV Indonesian isolate. Universal primer for Begomovirus, i.e. SPG1/SPG2 was used to amplify DNA fragment of Begomovirus which cover the TrAp and Rep gene. The amplicon of ± 912 bp in size was then cloned into pTZ57R/T plasmid vector by TA-cloning method to obtain plasmid recombinant pTZ-SLCCNV. The plasmid recombinant was successfully confirmed by PCR colony and DNA sequencing. The nucleotide sequences analysis of plasmid recombinant showed highest homology (98%) of the corresponding gene to pumpkin isolate of SLCCNV from Malaysia.

MORPHOLOGICAL CHARACTERS AND GENETIC VARIABILITY OF CHAMPACA IN BALI I Made Sukewijaya; Made Sudiana Mahendra; I Nyoman Rai; I Gede Rai Maya Temaja
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Bali community utilize champaca flower for offering materials and worship, besides beauty salon purposes, the SPA aromatic ingredients, essential oils, perfumes, cosmetics, and drugs. Various champaca plants in Indonesia have not been studied as one of Indonesia's biodiversity that can be used as excellent genetic resources (germplasm). The objective of the study was to determine the genetic diversity of champaca in Bali. The results revealed that 12 (twelve) champaca accession morphologically was characterized. All of accessions obtained from cultivation centers champaca in Bali. Based on the characteristic was observed by morphological characters i.e.: (a) Cempaka Putih Wilis (b) Cempaka Putih Pateh, (c) Cempaka Putih Patemon, (d) Cempaka Putih Sibang, (e) Cempaka Kuning Muda Petemon, (f) Cempaka Kuning Kecil Patemon, (g) Cempaka Kuning Besar Patemon, (h) (i) Cempaka Kuning Kecil Sibang, (j) Cempaka Kuning Tua Sibang, (k) Cempaka Kuning Muda Sibang, and (l) Cempaka Kuning Punah Sibang. Morphologically, champaca in Bali can be grouped into 4 clusters and therefore, and based on RAPD analysis champaca in Bali could be grouped into 5 clusters.

DETECTION AND ELIMINATION VIRUS ON POTATO (Solanum tuberosum L.) Debora Margareth; Ida Ayu Astarini; I Gede Rai Maya Temaja
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

This study aims to determine the symptoms of virus attacks on potato crops, identification of viruses that attack potato plants in Pancasari village, Bali, efectivity of thermotherapy technique to eliminate in vivo potato. The research was taken using purposive sampling technique and complete random program. Virus symptoms were observed on two potato fields. Serological test employed DAS-ELISA with PVX, PVS, PVY and PLRV antiserum. In vivo elimination virus were done by thermoteraphy in 37°C±1°C (4 days), 34°C±1°C (3 days) on 3 weeks and 33°C±1°C (4 days), 30°C±1°C (3 days) on 2 weeks. Results of the study at Pancasari Village Bali shown varied symptoms on potato leaves, such as mottle, mosaic, malformation, leafroll, witchesbroom. Through serological test, had identified viruses were 11.11% PVX, 55.56% PVS, 80% PVY and 11.11% PLRV which were infected farmer’s potato. In vivo thermotherapy was succeed decreasing PVS and PVY level during 2 weeks on 33°C±1°C (4 days) and 30°C±1°C (3 days).

APLIKASI TEKNOLOGI PEMBUATAN PUPUK ORGANIK PLUS UNTUK MENINGKATKAN PRODUKTIVITAS GAPOKTAN UMA DESA KABUPATEN I.G.R.M. temaja; G.N.A.S. Wirya; N.L.G. Sumardani
Buletin Udayana Mengabdi Vol 17 No 1 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

The short course on organic fertilizer plus was held on Thursday, April 20, 2017 at Simantri 376. There are32 members of Gapoktan Uma Desa participated in this program. The purpose of the short course is toprovide knowledge, understanding and skill to members of Gapoktan about increasing productivity throughthe utilization of bali cattle dung as an organic fertilizer that has higher economic value. The problem solvingmethod is to give theory and practice about formulating fermented organic fertilizer, Trichoderma on carriermedia and organic fertilizer plus. During the delivery of theory and practice the participants' responses werevery enthusiastic, so the discussion took place actively

TEKNIK DAN MANAJEMEN PRODUKSI BIBIT SAPI BALI DI SUBAK KACANG DAWA DESA KAMASAN KLUNGKUNG N.L.G. Sumardani; I.G.R. Maya Temaja; G.N.A. Susanta Wirya; N.M. Puspawati
Buletin Udayana Mengabdi Vol 16 No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Penyuluhan dan pelatihan mengenai manajemen produksi peternakan dan pemanfaatan Inseminasi Buatan (IB) pada ternak sapi bali telah dilaksanakan pada tanggal 9 Oktober 2016 di Simantri 451 Sedana Murti Desa Kamasan, Kabupaten Klungkung. Kegiatan ini diiukuti oleh 15 petani yang tergabung dalam kelompok petani Simantri 451 Sedana Murti. Hasil dari kegiatan ini adalah peningkatan produksi bibit sapi bali dengan menerapkan program IB. Metode yang digunakan dalam kegiatan ini meliputi diskusi mengenai pembibitan, manajemen kesehatan ternak, program IB, dan praktek langsung mengenai IB pada sapi bali. Dari kegiatan ini dapat disimpulkan bahwa peserta penyuluhan dan pelatihan sangat antusias dalam menerima materi mengenai IB pada sapi bali.

PELATIHAN PENGENDALIAN PENYAKIT TUNGRO DAN BLAS PADA TANAMAN PADI DI SUBAK BASANGKASA IGRM TEMAJA; M SUDANA; IP SUDIARTA; GNA SUSANTA WIRYA; NM PUSPAWATI
Buletin Udayana Mengabdi Vol 14 No 1 (2015): April 2015
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Training on tungro and blast diseases was held at Subak Basangkasa, Kerobokan village, Badung regency onApril 27, 2014. The activities was conducted to educate farmers how to control tungro and blast diseases. Themethods used in this activity were lectures, demonstration and practice in the paddy field. The training wasattended by 30 participants from local farmer groups of Subak Basangkasa. Based on post test, more than 90% ofthe farmers managed to answer the questions about tungro and blast diseases, including pathogens, symptoms ofdiseases, factors affecting the growing of diseases as well as the control of diseases. The data indicated on the finalof training all participants completely understand about the topics. All participants participated enthusiasticallyand hope they have the next intensive training again.Key words :disease control, tungro disease, blast disease, trainin

Co-Authors A. A. GEDE PUTRA ADHITYA Abd. Rasyid Syamsuri Ayu Rahmawatiningsih Chiharu Hongo Debora Margareth Dewa Ngurah Suprapta Dewa Nyoman Darmayasa G. SUASTIKA Gede Bayu Rahanatha GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Gusti Ngurah Alit Susanta Wirya I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I Dewa Nyoman Nyana I Dewa Nyoman Nyana I Gede Rian Pramarta I Komang Candra Giri Prayoga I Made Angga Prayoga I MADE SUDANA I Made Sudana I Made Sudana I MADE SUDARMA I Made Sukewijaya I NYOMAN RAI I PUTU WIRYA SUPUTRA I WAYAN WIKA CENDANA PUTRA IDA AYU ASTARINI IDA BAGUS GDE PRANATAYANA Ifa Manzila KETUT AYU YULIADHI Khairun Nisak Syahdu Khamdan Khalimi l MADE SUDARMA LASTRI APRIANI M SUDANA M. Sudiana Mahendra Masahiro Shishido Muhammad Ikhsan Nulzaen Ni Luh Gde Sumardani Ni Luh Gede Sriyanti Ni Luh Putu Citra Innosensia NI LUH PUTU CITRA INNOSENSIA Ni Made Puspawati NI NENGAH DARMIATI Ni Nengah Dwi Fatmawati Ni Nengah Putri Adnyani Ni Nyoman Alit Purwaningsih NI NYOMAN ARI MAYADEWI Ni Nyoman Kerti Yasa Ni Putu Widyami Yanthi Nyoman Bintang Kartika Sari Putu Sudiarta Reymas M.R. Ruimassa RIZWAN FEBIANTA BRAHMANA Sari Nurulita SH. HIDAYAT SONIA ASHA HASARI SUSAN CAMILA FOEH TRISNA AGUNG PHABIOLA U. KARTOSUWONDO Valente Quintao Wahyu Hidayati Yuyun Fitriani

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