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Pemberian Fikosianin Spirulina Meningkatkan Jumlah Sel Darah, Aktivitas Fagositosis, dan Pertumbuhan Ikan Kerapu Bebek Juvenil (ADMINISTRATION OF SPIRULINA PHYCOCYANIN ENHANCES BLOOD CELLS, PHAGOCYTIC ACTIVITY AND GROWTH IN HUMPBACK GROUPER JUVENILE) Woro Hastuti Satyantini; Sukenda .; Enang Harris; Nur Bambang Priyo Utomo
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to investigate effects of Spirulina phycocyanin on the total  blood cell count,phagocytic activity, and growth of humpback grouper fish, Cromileptes altivelis juvenil.  Fishes were fedwith a diet containing   0, 150, 250, 350 dan 450 mg  phycocyanin per kg diet for four weeks and eachtreatment was triplicates.  Initial body weight  of  grouper was  8.46 ± 0.22 g with a density of 10 fish per56 litre volume. The total count of  erythrocytes and leucocytes increased until the fourth week of rearingperiod. The highest of total erythrocyte and leucocytes were observed in fish treated with 150 mg phycocyaninper kg diet ( 13.17 x  105 cells/mm3 and 8.93 x 105 cells/mm3 respectively) which were not significantlydifferent (P>0.05) to those treated with 250 mg phycocyanin per kg diet. The total leucocytes and phagocyticactivity of fish fed diet containing  250 mg phycocyanin  per kg diet (8.49 x 105 cells/mm3 and 59.67%respectively) were significantly higher  (P <0.05) to those of control group. The highest of final weight(Wt=14.32 g) and weight growth (G=5.89g) and lowest of feed conversion ratio (FCR=1.13) were obtainedin fish treated with  250 mg phycocyanin per kg diet which were  significantly  higher  (P <0.05) than thosefed control diet. The data showed that  the addition of  phycocyanin 250 mg/kg diet enhances the totalleukocyte count, phagocytic activity and the growth of humpback grouper juvenil.
Enhancement of Nonspecific Immune Response and Growth Performance of Litopenaeus vannamei by Oral Administration of Nucleotides (PENINGKATAN RESPONS IMUN NONSPESIFIK DAN PERFORMA PERTUMBUHAN LITOPENAEUS VANNAMEI MELALUI PEMBERIAN NUKLEOTIDA SECARA ORAL) Henky Manoppo; Sukenda .
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research evaluated the nonspecific immune responseand growth of Litopenaeus vannamei fednucleotide diet.  In Laboratory, juveniles were reared in two groups of glass aquaria, each with threereplications.  Shrimps in group one were fed nucleotide diet and in group two were fedpellet four consecutiveweeks. Total Haemocyte Count and Phenoleoxydase activity were evaluated at the end of feeding whilegrowth was measured at two weeks interval. At the end of feeding, shrimps were intramuscularlyinjectedwith Vibrio harveyi  0.1x106 cfu.shrimp-1.  In tambak, juveniles were raised in two groups of net cages(hapa), each with three replications. One group was fed nucleotide diet while the other wasfed pellet forfour weeks. Total Haemocyte Count of shrimp fed nucleotide diet significantly increased up to 87% higherthan shrimps fed pellet.  Phenoleoxydase activity of shrimp fed nucleotides diet also increased isignificantlyas compared to shrimp fed pellet (p=0.02). Higher resistance and growth were observed in shrimp fednucleotide diet. In tambak, weight gain of shrimp fed nucleotide was 35.75% greater than shrimp fedpellet. Survival rate (83.24%) was higher than shrimp fed pellet (81.71%).  As conclusion, oral administrationof nucleotide at 400 mg.kg-1 diet could enhancethe nonspecific immune response, resistance, and growth ofL. vannamei.
Kandidat Vaksin Potensial Streptococcus agalactiae untuk Pencegahan Penyakit Streptococcosis pada Ikan Nila (Oreochromis niloticus) (POTENTIAL VACCINE CANDIDATE OF STREPTOCOCCUS AGALACTIAE FOR PREVENT STREPCOCOCOSIS ON NILA TILAPIA (OREOCHROMIS NILOTICUS) Esti Handayani Hardi; Sukenda .; Enang Harris; Angela Mariana Lusiastuti
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The effectiveness of Streptococcus agalactiae vaccine in tilapia (Oreochromis niloticus) was evaluatedfor prevention of streptococcal disease. The vaccine was prepared using formalin-killed whole cell andconcentrated extracellular products/ECP (62.3 and 55.8 kDa) of â-haemolityc isolate and 62.3; 55,8 and51.8 kDa protein of  non-haemolityc ECP of S. agalactiae.  Vaccination and challenged (103 colony-formingunits (CFU)/fish of â-haemolityc and 105 CFU/fish of non-haemolityc S. agalactiae) trial was conducted byintraperitonial (IP) injection into fish with average body weight of 15 g.  Fish were vaccinated with wholecell, ECP and mixed (whole cell and ECP) vaccine.  Tilapia vaccinated with whole cell of â-haemolitycisolate had a relative percent survival (RPS) rates higher than those of ECP â-haemolityc vaccine. However,fish  vaccinated with mixed (whole cell and ECP) of â-haemolityc has a better protection rates as comparedto those of two type of S. agalactie infection. Whereas those vaccinated with mixed (whole cell non-haemolitycand ECP of â-haemolityc) vaccine has protection rate of 79% from â-haemolityc and 42% from non-haemolitycinfection.  Tilapia vaccinated with whole cell of non-haemolityc was only able to protect fish from non-haemolityc infection and was unable to protect fish from other types.  Tilapia vaccinated with ECP non-haemolityc had a worse RPS than others vaccines in which mix whole cell and ECP vaccine of non-haemolitychad a protection 50-56% from  S. agalactiae infection. Whereas vaccinated with mixed (whole cell â-haemolityc and ECP of non-haemolityc) vaccine showed a better to protect from â-haemolityc than non-haemolityc infection.  It showed thatvaccination with mixed (whole-cell and extracellular product)  vaccineof S. agalactiae â-haemolityc  was more effective to protect tilapia against Streptococcosis.