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Uji Efek Kelasi Ekstrak Etanol Daun Mangifera foetida pada Feritin Serum Penderita Talasemia di Rumah Sakit Cipto Mangunkusumo, Tahun 2012 Anggi Pohan; Erni H Purwaningsih; Adisti Dwijayanti
eJournal Kedokteran Indonesia Vol 1, No 1 (2013): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Efek Antioksidan Kombinasi Ekstrak Etanol Acalypha indica dan Centella asiatica pada Fungsi Hati Tikus Pascahipoksia Sistemik Agnes Frethernety; Melva Louisa; Novi S. Hardiany; Adisti Dwijayanti; Erni H. Purwaningsih
eJournal Kedokteran Indonesia Vol 3, No 2 (2015): Agustus
Publisher : Faculty of Medicine Universitas Indonesia

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Hipoksia adalah defisiensi suplai oksigen ke dalam sel atau jaringan karena gagalnya sistem respirasi yang membawa oksigen sehingga mengakibatkan kerusakan jaringan. Hati merupakan organ yang sensitif terhadap hipoksia. Tanaman Acalypha indica dan Centella asiatica memiliki efek antioksidan dan dapat melindungi banyak organ dari hipoksia. Tujuan penelitian ini menganalisis pengaruh pemberian kombinasi ekstrak etanol A.indica dan C.asiatica pascahipoksia sistemik terhadap fungsi hati, stres oksidatif dan aktivitas antioksidan hati. Sebanyak 28 tikus spraquedawley dibagi secara acak menjadi 7 kelompok. Kelompok kontrol adalah perlakuan tanpa hipoksia, perlakuan enam kelompok lainnya pascahipoksia 7 hari diberikan zat uji sebagai berikut: air, kombinasi dosis 1 dan 2, dosis tunggal A.indica, dosis tunggal C. asiatica dan dosis tunggal vitamin C selama 7 hari. Parameter yang diukur adalah aktivitas ALT dan AST, kadar MDA, rasio GSH/GSSG. Tidak ada perbedaan aktivitas ALT dan AST yang bermakna pada semua kelompok. Kadar MDA meningkat pada kelompok pascahipoksia 7 hari dibanding kontrol (p=0,007). Kelompok kombinasi 1 memiliki MDA yang rendah, rasio GSH/GSSG dan aktivitas SOD yang meningkat dibandingkan kelompok pascahipoksia 7 hari. Pemberian zat uji kombinasi 1 memiliki efek perlindungan pada hati tikus terhadap pascahipoksia 7 hari melalui mekanisme stres oksidatif dan antioksidan. Kata kunci: Acalypha indica, Centella asiatica,hipoksia sistemik, antioksidan The Effect of A. Indica and C.Asiatica Ethanol Extract Combination on Rats Liver Function Post-Hypoxic Condition Abstract Hypoxia occurs due to the deficiency of oxygen supply to cells or tissue caused by the failure of the respiratory system that carries oxygen which results in cell or tissue damage. Liver is an organ sensitive to hypoxia. Acalypha indica and Centella asiatica are proven to have antioxidant effects and can protect many organs from hypoxic conditions. This study was aimed to analyse the effect of A. indica and C.asiatica ethanol extract combination on post-hypoxic condition towards liver function, oxidative stress and antioxidant activity of the liver. Twenty-eight spraque-dawley rats divided randomly into 7 groups. Control group was treated without hypoxia and the other six groups were given substances as follows on the 7th day of hypoxia: water, combination of dose 1 and 2, single dose of A. indica, single dose of C.asiatica, and single dose of vitamin C for 7 days. Parameters measured were activities of ALT and AST, MDA, GSH / GSSG ratio and SOD activity. There was no significant difference in the activity of ALT and AST in all groups. MDA levels was increased in the 7-days post-hypoxic group compared to control (p=0,007). The combination 1 group had low MDA and increased GSH/GSSG ratio and SOD activity compared to the post-7days-hypoxic group. The substance of combination 1 has protective effect on the rats’ liver on post-7-days-hypoxic through oxidative stress and antioxidant mechanisms. Keywords: Acalypha indica, Centella asiatica, systemic hypoxia, antioxidant
Comparison of Antioxidant Activity and Tannin Level of Pegagan Extract to Commercially Available Product Achmad Zaki Maulidzy; Adisti Dwijayanti
eJournal Kedokteran Indonesia Vol 4, No 1 (2016): Vol 4, No. 1 (2016): April
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Centella asiatica (pegagan) is a well-known traditional medicinal plant that has been processed into many commercial herbal products. Those herbal products were claimed to have antioxidant effects which were beneficial to overcome free radicals in the body. Antioxidant activity of those commercial products compared to the extracts has not been studied yet. The aim of this study is to compare the antioxidant activity and tannin levels of water and ethanol extracts of C.asiatica compared to three herbal products of C.asiatica. Water extract is made by infusion while ethanol extract is made by maceration. Three brands of C.asiatica products were obtained from the market. Levels of antioxidants were measured by comparing the EC50 value using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Tannin levels were measured qualitatively and semiquantitatively using FeCl3. Results showed that antioxidant activity of the water and ethanol extract of C.asiatica wer significantly higher than the commercial products (p≤0.05). Tannin’s semiquantitative test showed that all samples contained tannins but the amount was very low.
Implementation of Electronic Prescribing Siti Farida; Desak Gede B. Krisnamurti; Rani Wardani Hakim; Adisti Dwijayanti; Erni H. Purwaningsih
eJournal Kedokteran Indonesia Vol 5, No 3 (2017): Vol 5, No. 3 (2017): Desember
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Medication errors often occur in general practice and in hospitals partly due to an error in the prescription or prescribing error because of incorrect medical decisions that affect patient safety and healthcare quality. Therefore, it is necessary to prevent and reduce such errors, among others, by writing an electronic prescribing (e-prescribing). This review will address the results of studies on the prevalence of prescription errors and the benefits of implementing e-prescriptions to avoid misinterpreting doctor’s handwriting on prescription papers and excessive drug prescribing, efficiency of drug preparation time at pharmacies, and the ability of e-prescribing manage databases that can analyze the history of drug allergy, proper dosage and potential drug interactions that harm patients. Moreover, it also discusses the implementation strategy, infrastructure support and government monitoring system (Food and Drug Supervisory Agency) regularly. Implementation of e-prescribing with appropriate strategies will have a positive impact on healthcare quality and patient safety.
Penyuntikan Human Wharton’s Jelly Mesenchymal Stem Cells terhadap Perbaikan Fungsi Testis pada Tikus Tua Fisiologis (HUMAN WHARTON’S JELLY MESENCHYMAL STEM CELLS INJECTION AMELIORATE TESTICULAR FUNCTION ON PHYSIOLOGICAL AGING MALE RATS) Alif Iman Fitrianto; Adkhilni Utami; Wining Astini; Adisti Dwijayanti; Frans Dhyanagiri Suyatna; Kelvin Yaprianto; Indra Bachtiar; I Ketut Mudite Adnyane; Arief Boediono
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The most common therapy on men who suffered fertility decline due to aging was called “T Therapy”, but that’s therapy has long-term risks of sexual dysfunction, metabolic syndrome, prostate, and cardiovascular system. Stem cells are an alternative therapy can be used for ameliorate testicular fuction because of their ability to differentiate into various cell types. The aim of this study was to evaluate the injection of hWJ-MSC in physiologic aging male rats on testicular function. This study was used 3 young male rats (8-12 weeks) and 6 physiological aging male rats (22-24 months) which divided into 3 groups, (i) the young rats, (ii) physiological aging male rats, and (iii) physiological aging male rats that injected with hWJ-MSCs. The young rat group did not give any treatment, physiological aging male rats received NaCl (0.9%) 0.4 mL, and the treatment group received 1x106 cells/kg BW of hWJ-MSCs. The observations were performed on the macroscopical and histological analysis. The result indicates that the younger group had the lowest body weight (154.6 g) and the percentage of the testis weight on the body weight was highest (2.2%) compared to the other groups (P>0.05). The physiological aging rats group had the smallest tubule (9726.9 ìm2) with a largest interstitial area (1117.1 ìm2) compared the other groups (P>0.05). After injection of hWJ-MSC, the tubule area became wider followed by narrowing of the interstitial area (P>0.05). The difference in the body weights is due to the different age of the rat. Improvement of tubule area and interstitial area due to the ability of hWJ-MSCs to improve spermatogenic cells within the tubule. Injection of hWJ-MSCs has been shown to increase fertility in aging rats.
Perpanjangan Usia Tikus Tua Setelah Xenotransplantasi Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Elpita Tarigan; Adisti Dwijayanti; Frans Dhyanagiri Suyatna; Indra Bachtiar; Sandy Qlintang; I Ketut Mudite Adnyane; Arief Boediono
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.261 KB) | DOI: 10.19087/jveteriner.2020.21.4.539

Abstract

Currently, mesenchymal stem cells (MSCs) for implementing regenerative medicine in aging become interest in medical research science, especially in degerative disease and other aging problems. This research was aimed to determine the effectiveness of hUC-MSCs on inhibiting the aging process through the lifetime of the rat and the effect of intravenous administration of hUC-MSCs in phisiologycally aging female rat on the blood analysis. This study was used 40 aged female rats with 29-30 months of age divided into four groups with 10 rats each. The control rat group was given physiological NaCl (0.9%) 0.5 mL, and the treated rat group was given hUC-MSCs 1x107 cells/kg body weight in 0.5 mL NaCl 0.9%, wasinjected intravenously in caudo lateralis tail vein with stratified frequency; one time injection (SC1), three times injections (SC3) and five times injections (SC5). Perifer blood was collected from retro-ortbital sinus vein 30 days before and after injection of hUC-MSCs for hematology and blood chemistry analysis. Based on the results were obtained, it indicated that hUC-MSCs increased the survival of aging rat werein treatment group, life span of rats was extended up to 40 months compared to the average life of control rat aged up to 34±2 months. The injection of hUC-MSCs 1x107 cells/kg of body weight with one, three andfive times injection were affected to blood profiles and blood chemistry with correlation were low. The conclusions are hUC-MSCs extend the lifespan of aging rat and were affect the blood in general but in normal range of aging rat, affect in ALT and creatinin as tissue repair and tolerated by aging rat.
Follicular Development of Aged Rats Ovarian Injected Human Umbilical Cord Mesenchymal Stem Cells Resti Rahma Dianti; Alif Iman Fitrianto; Adkhilni Utami; Wining Astini; Adisti Dwijayanti; Frans Dhyanagiri Suyatna; Kelvin Yaprianto; Indra Bachtiar; Aryani Sismin Satyaningtijas; Adi Winarto; Arief Boediono
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 1, JANUARY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i1.6889

Abstract

Female reproductive system showing the fastest signs of aging. The ovarian aging characterized by a decrease in follicular development. Stem cells are undifferentiated cells and can form a variety of different cells as the foundation of tissues and organs. Previous studies reported that Bone Marrow Mesenchymal Stem Cells (BM-MSCs) transplantation can restore follicular development in damaged ovarian rats. This study aimed to analyze the number of follicular development in aged rats and to analyze the capability of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) to improving follicular development in aged rats. This study used 3 mature rats (4 months old), and 9 nine aged rats (22-24 months old), Spraque Dawley (SD) strain. They were divided into four groups. The first and the second group was mature rats and aged rats without injection. The third and the fourth group was aged rats injected hUC-MSCs dose 106 cells/kgBW and hUC-MSCs dose 107 cells/kgBW. The injection carried out 4 times at 3-month intervals. The parameters observed were follicular development and homing image of hUC-MSCs in ovarian tissue. The results showed that the number of follicular developments in aged rats 22-24 months decreased significantly compared to mature rats 4 months old. Injection of hUC-MSCs at dose 106 cells/kgBW and 107 cells/kgBW did not increase follicular development in aged rats. hUC-MSCs did not found in ovarian tissue. It could be concluded that aged rats 22-24 months old no longer productive indicated from the number of follicular developments and corpus luteum decreased. The injection of hUC-MSCs intravenously did not indicate an improvement of follicular development in aged rats 22-24 months old.
Perubahan Parameter Biokimia, Histopatologi Ginjal Tikus Spraque Dawley Pascahipoksia Oleh Ekstrak Akar Acalypha indica dan Herba Centella asiatica Nurfitri Nurfitri; Erni Hernawati Purwaningsih; Vivian Soetikno; Adisti Dwijayanti; Novi Silvia Hardiany
Jurnal Sains Farmasi & Klinis Vol 5, No 3 (2018): J Sains Farm Klin 5(3), Desember 2018
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (731.281 KB) | DOI: 10.25077/jsfk.5.3.160-168.2018

Abstract

Hipoksia kronik merupakan salah satu penyebab penyakit ginjal akibat peningkatan pembentukan Reactive Oxygen Species (ROS)  dalam sel. Kombinasi ekstrak akar Acalypha indica 250 mg/KgBB (AI250) dan Centella asiatica 150 mg/kgBB (CA150) memiliki efek neuroterapi pada tikus Spraque Dawley pascahipoksia. Penelitian dilakukan untuk membuktikan manfaat kombinasi ekstrak etanol dan/atau ekstrak tunggalnya dapat memperbaiki kerusakan ginjal tikus pascahipoksia melalui mekanisme antioksidan. 28 tikus jantan dikelompokkan dalam 7 kelompok: kontrol normal; kontrol hipoksia+air; hipoksia+(AI200+CA150); hipoksia+(AI250+CA100); hipoksia+AI250; hipoksia+CA150; hipoksia+vitamin C. Hipoksia selama 7 hari dalam hypoxic chamber berisi O2 10% dan N2 90%, 1 atm. Setiap kelompok diberi perlakuan selama 7 hari. Pada akhir studi hewan diterminasi. Darah dan organ ginjal diambil untuk pemeriksaan biokimia dan histopatologi.Kombinasi  (AI250+CA100) menurunkan kadar MDA ginjal dan plasma secara bermakna dibandingkan kontrol hipoksia (p=0,001 dan p=0,021) dan AI250 (p=0,003 dan 0,043). Kombinasi AI250+CA100 terjadi penurunan ekspresi relatif mRNA HIF-1α (p=0,014), kadar urea plasma (p=0,001) dan perbaikan lesi intra-glomerulus p=0,013.Kesimpulan: Kombinasi (AI250+CA100) dan tunggal AI250 memiliki aktivitas antioksidan terbaik dalam mencegah kerusakan ginjal pascahipoksia, secara biokimiawi dan histopatologinya.
Brain Derived Neurotrophic Factor Levels in Aged Rats Post-Systemic Human Mesenchymal Stem Cell Administration adisti dwijayanti
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 2 (2018): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.863 KB) | DOI: 10.20473/jscrte.v2i2.11895

Abstract

Brain-Derived Neurotrophic Factor (BDNF) levels were affected by aging. Brain BDNF levels were known to decrease along with advanced age thus correlated with any diseases such as cognitive impairment and Alzheimer. Mesenchymal Stem Cell (MSC) is one of the potential modalities actively investigated against age-related diseases. This study evaluated the effect of human MSC administration to brain BDNF levels in aged rats. Intravenous injection of 10 million per body weight human MSC were given four times in 3 months interval to 22-24 months old female and male Spraque–Dawley rats. As control group, aged rats were injected by normal saline at the same volume and frequencies. Moreover, young 3-6 months rats also examined as negative control.  By the end of the experiment, we analyzed three rats from each group. Brain BDNF levels were measured by enzyme-linked immunosorbent assay and normalize to the protein levels. One-way ANOVA and LSD post hoc analysis was performed to compare the differences between groups. BDNF levels in male appeared similar between young, aged, and MSC treated groups. Meanwhile, control aged female groups had significantly lower BDNF levels compared to young (p = 0.019) and MSC-treated aged rats (p = 0.001). There was no difference of BDNF levels between young and MSC-treated aged in female rats (p = 0,068). Both sex had similar BDNF levels (p = 0.249) in control-aged groups. In contrast, female young and MSC-treated aged rats achieved significantly higher BDNF levels (p = 0.009 and p <0.001) compared to the male groups, respectively. These results suggest that human mesenchymal stem cell intravenous injection can increase brain BDNF levels in female aged rats.