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Raden Wasito
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Veterinary

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Gambaran Histopatologi Insang Ikan Mas di Daerah Endemik Koi Herpesvirus (HISTOPATHOGIC FINDINGS OF GILLS OF THE COMMON CARPS IN THE ENDEMIC AREA OF KOI HERPESVIRUS) Raden Wasito; Hastari Wuryastuti; Bambang Sutrisno
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Koi herpesvirus (KHV)  is the cause of  a lethal disease that affects common carp (Cyprinus carpio)  andkoi (Cyprinus carpio koi). Although, it has been reported that common carps could act as carriers for KHV,their histopathologic findings, especially the gills  have not been identified up to now. In the present study,12  normal, healthy looking common carps including their gills were collected from the endemic area ofKHV in  Sleman, Yogyakarta. All fish were necropsied and the gills were collected and fixed in 10% bufferformalin. Then, the gills were processed histopathologically using routine hematoxyline-eosin stain andexamined under the microscope. Histopathologic examination of the gills exhibited an apparent infiltrationof inflammatory cells, especially lymphocytes. The large oval to polygonal basophilic cells containing largeintranuclear inclusion bodies were also identified. Gills epithelial cells show mass hyperplasia and adhesionwith necrotic changes. Thus, results of this study has led to a reasonable conclusion that KHV infection ispresent in the normal, healthy common carps. One possibility is those KHV  are live viruses (carriers forKHV) and might could act as a source of infection is being discussed.
Identifikasi Koi Herpesvirus dengan Uji Imunopatologi Imunohistokimia Streptavidin Biotin pada Ikan Mas Karier (IDENTIFICATION OF KOI HERPESVIRUS USING IMMUNOPATHOLOGIC IMMUNOHISTOCHEMISTRY OF STREPTAVIDIN BIOTIN IN THE COMMON CARP CARRIERS) Raden Wasito; Hastari Wuryastuti; Bambang Sutrisno
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In  managing the koi herpesvirus (KHV) outbreaks as a routine national program in Indonesia, testingbased on biotechnology, such as  immunopathologic immunohistochemical approach(es) using antibodythat is safe, rapid  and accurate need to be applied. This will hopely assist the Government of Indonesianin improving and enhancing the sustainability of national animal proteins program. The present studywas aimed to develop and apply the immunopathologic immunohistochemistry of streptavidin biotin (IHCSB) for detection of KHV in the apparently normal carps. The gills from 48 common carps  (Cyprinuscarpio) that appear to be healthy were prepared for  DNA-based KHV  by IHC SB.  Common carps werecollected from fish farms which had an outbreak of KHV in 2008-2009 in Yogyakarta.  All fish werenecropsied. The gills were processed histopathologically and then stained for IHC SB with monoclonalantibody anti-KHV. We demonstrated that all of the fish gills were positive for KHV antigen. Thus, it isconcluded that method is useful and consistent, very sensitive and rapid, and is a reliable method to beapplied for field condition to detect antigen KHV in the gills of normal, healthy looking carps.   In addition,and more importantly, the fish can act as a source of KHV (carriers for KHV) and may result in the spreadof diseases among susceptible fish.
Co-Authors Bambang Sutrisno Hastari Wuryastuti