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PENGARUH TEMPERATUR TERHADAP PATOGENESITAS OOSISTA Eimeriu tenella PADA AYAM PEDAGING Ery Sri Rohayati; Dewi Rahmawati; Bambang Sutrisno
Jurnal Sain Veteriner Vol 29, No 1 (2011): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Koksidiosis adalah problem industri ayam yang pencegahan penyakit tersebut masih menjadi masalah di Indonesia. Penelitian ini bertujuan untuk mengetahui pengaruh temperar terhadap patogenesitas oosistaEimeria tenella pada ayam pedaging. Sebanyak 15 ekor day old chick (DOC) pedaging umur 17 hari dibagi menjadi tiga kelompok, masing-masing terdiri dari 5 ekor ayam. Setiap ayam dari kelompok A, B, dan C,masing-masing kelompok diinfeksi secara oral dengan 5000 oosista yang ditretmen dengan temperatu 4"C selama 17 hari, dengan 5000 oosista yang ditretmen pada temperatu 50"C selama 15 hari dan dengan 5000oosista yang ditretmen dengan temperatur ruang(27"C). Ayam dinekropsikan pada hari ke-5 dan ke-7 setelah infeksi untuk dilihat gambaran histopatologinya. Gambaran lesi sekum dianalisis dengan metode deskriptif, sedangkan derajat lesinya dianalisis dengan metode Rank test. Hasil penelitian menyimpulkan bahwa temperaturberpengaruh terhadap patogenesitas oosista E . tenella dan untuk mematikano osista E . tenella disarankan dengan pemanasan 50 "C selama 15 hari.Kata kunci: koksidiosis, Eimeria tenella, lesion score, gambaran histopatologi

STUDI PERKEMBANGAN DAN SITOPATOLOGI Eimeria tenella PADA MEMBRANCHORIOALLANTOIS Bambang Sutrisno; Joko Prastowo
Jurnal Sain Veteriner Vol 21, No 1 (2003): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Penelitian ini bertujuan untuk mengetahui perkembangan dari siklus hidup Eimeria tenella gambaran histopatologik membran chorioallontois sebagai akibat infeksi sporosista dan oosista Eimeria tenella, untuk mengetahui kemungkinan penggunaan membran chorioallantois sebagai medium k-ultur Eimeria tenella. Penelitian ini menggunakan 50 butir telur ayam berembrio umur 10 hari yang dibagi ke dalam 5 kelompok, masing-masing 10 butir. Kelompok 1, telur diinfeksi dengan 5.000 oosista, kelompok II diinfeksi dengan 10.000 oosista, kelompok 111 diinfeksi dengan 25.000 sporosista, kelompok IV diinfeksi dengan 50.000 sporosista dan kelompok V diperlukan sebagai kontrol. Telur-telur tersebut diinkubasi pada suhu 41°C selama 6 hari. Mulai hari ke 2 setelah infeksi, dua telur dad masing-masing kelompok diperiksa dengan membuka kerabangnya, membran chorioallantois diamati untuk melihat perubahan makroskopik dan histologik. Hasil yang didapat adalah kelompok I dan II tidak ada perbedaan dan oosista tidak berkembang bahkan mengalami nekrosis. Kelompok III dan /V menunjukkan perkembangan di dalam membran chorioallantois, hari ke 2 setelah infeksi sel epitel membesar dan terisi oleh sporozoit, hari ke 3 setelah infeksi sudah ada skizon generasi I, hari ke 4 setelah infeksi sel-sel epitel membesar dan ada stadium makrogamet, skizon generasi II dan zigot, dan had ke 5 setelah infeksi sudah terdapat stadium oosista. Inokulasi sporosista Eimeria tenella pada membran chorioallantois dapat berkembang menjadi stadium berikutnya, sedang inokulasi oosista Eimeria tenella pada membran chorioallantois tidak menunjukkan perkembangan. Membran chorioallantois dapat digunakan sebagai medium kultur Eimeria tenella.Kata kunci : Chorioallantois, Eimeria tenella, Skizon , Makrogamet

Lesi Patologik Organ dan Jaringan Ikan Nila (Oerochromis niloticus) yang Diinfeksi Bakteri Staphylococcus sp. Bambang Sutrisno; Yuli Purwandari K.
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Lesi Patologik Organ Dan Jaringan Ikan Nila (Oerochromis nilaticus) Yang Diinfeksi Bakteri Staphylococcus sp. Bambang Sutrisno; Yuli Purwandari K.
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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STUDI HISTOPATOLOGIS TUMOR PADA HEWAN KESAYANGAN AND WILD ANIMAL Sitarina Widyarini; Sugiyono Sultan; Yuli Purwandari Kristiangingrum; Bambang Sutrisno
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70506

Penelitian ini dilakukan untuk mengamati gambaran histomorfologis (berdasarkan pada histogenesis dan sifat pertumbuhan tumor), pada berbagai sampel tumor dari hewan kesayangan (anjing dan kucing) dan wild animals (beruang) koleksi Departemen Patologi Fakultas Kedokteran Hewan Universitas Gadjah Mada. Duapuluh satu sampel (22) dari organ yang diduga jaringan tumor yang diperoleh dari Dokter Hewan Praktisi maupun Rumah Sakit Hewan selama tahun 2019 digunakan dalam penelitian ini. Semua sampel tersimpan dalam kontener berisi larutan formalin 10%. Selanjutnya sampel diproses dan diwarnai dengan Hematoxylin-Eosin. Pengamatan terhadap histogenesis dan sifat pertumbuhan tumor dilakulan dengan menggunakan mikroskop cahaya dengam pembesaran 200X dan 400X dan selanjutnya dilakukan dokumentasi dengan pengambilan foto. Analisis dilakukan secara deskriptif dengan membandingan dengan referensi histomofologis tumor pada hewan. Hasil penelitian memperlihatkan bahwa selama tahun 2019, jumlah sampel tumor kulit dilaporkan paling banyak 63.63% (14/22), diikuti dengan jumlah sampel tumor pada kelenjar mammae 22,72% (5/22), liver-duktus biliverus 18.18% (4/22), tulang 4,54% (1/22) dan metastasis paru-paru dan metastasis paru-paru dari jaringan tulang sejumlah 4,54% (1/22). Hewan asal tumor paling banyak ditemukan pada kucing dan anjing masing-masing 59% dan 34%. Hasil pengamatan terhadap gambaran histomorfologis ditemukan adenokarsinoma mammae (tubulopapillary carsinoma, solid adenocarsinoma, lipid-rich adenokarsinoma), fibroadenomatous hiperplasia mammae, karsinoma sel skuamosa, papiloma, adenoma kelenjar hepatoid, melanositosis, fibrosarkoma, kolangiokarsinoma dan osteosarkoma.

Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Yuli Purwandari Kristianingrum; Charles Rangga Tabbu; Bambang Sutrisno; Sitarina Widyarini; Kurniasih .; Tri Untari; Asmarani Kusumawati
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.278 KB)

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.

Identifikasi Leptin pada Kesembuhan Luka Tikus yang Diberi Pakan Lemak Tinggi dan Aplikasi Zinc Topikal (LEPTIN IDENTIFICATION ON WOUND HEALING OF RAT GIVEN HIGH FAT DIET AND TOPICAL ZINC APPLICATION) Devita Anggraeni; Dhirgo Adji; Bambang Sutrisno
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.099 KB)

Leptin is a hormone produced by adipocytes and play an important role in wound healing. The objectiveof this research was to study the role of leptin in wound healing in mice experimentally given high fat dietsand its correlation to zinc. Thirty two male Sprague Dawley rats at three months of age were used in thisstudy. Rats were randomly allotted into four groups (A,B,C and D) of 8. Rats in group A and B were fednormal diet, while rats in group C and D were fed high fat diet. After two months of treatment, skin incisionsurgery was performed at the back side of the rat. Incision wound was closed with single interruptedsuture. The wound of rats in group A and C were treated with vaseline, while in the group B and D weretreated with zinc 10%. One day after surgery, blood sample were collected frpm four rats from each groupand analysed for leptin (Rat leptin ELISA Int, Genway Biotech Inc). Wounded skin from all animals werealso taken for histopathological examination (Haematoxylin and Eosin). Three days after the surgery, thesame analysis were done for the remaining rats. Leptin level was analyzed statistically using ANOVA forfactorial experiment, while histopathologic analysis was done descriptively. The results showed that theleptin level was significantly affected by time (P<0.05), as leptin level in blood at three days after surgerywas significantly lower than the level at one day after surgery. Meanwhile, histopathological examinationshowed that the percentage of epidermal closure in animals in group A,B,C,and D were 75%, 100%, 25%and 75%, respectivelly. Therefore, it was concluded that topical application of zinc might have significanteffect on the wound healing of the rats fed normal diets as well as these that given a high fat diet.

Gambaran Histopatologi Insang Ikan Mas di Daerah Endemik Koi Herpesvirus (HISTOPATHOGIC FINDINGS OF GILLS OF THE COMMON CARPS IN THE ENDEMIC AREA OF KOI HERPESVIRUS) Raden Wasito; Hastari Wuryastuti; Bambang Sutrisno
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.885 KB)

Koi herpesvirus (KHV) is the cause of a lethal disease that affects common carp (Cyprinus carpio) andkoi (Cyprinus carpio koi). Although, it has been reported that common carps could act as carriers for KHV,their histopathologic findings, especially the gills have not been identified up to now. In the present study,12 normal, healthy looking common carps including their gills were collected from the endemic area ofKHV in Sleman, Yogyakarta. All fish were necropsied and the gills were collected and fixed in 10% bufferformalin. Then, the gills were processed histopathologically using routine hematoxyline-eosin stain andexamined under the microscope. Histopathologic examination of the gills exhibited an apparent infiltrationof inflammatory cells, especially lymphocytes. The large oval to polygonal basophilic cells containing largeintranuclear inclusion bodies were also identified. Gills epithelial cells show mass hyperplasia and adhesionwith necrotic changes. Thus, results of this study has led to a reasonable conclusion that KHV infection ispresent in the normal, healthy common carps. One possibility is those KHV are live viruses (carriers forKHV) and might could act as a source of infection is being discussed.

Identifikasi Koi Herpesvirus dengan Uji Imunopatologi Imunohistokimia Streptavidin Biotin pada Ikan Mas Karier (IDENTIFICATION OF KOI HERPESVIRUS USING IMMUNOPATHOLOGIC IMMUNOHISTOCHEMISTRY OF STREPTAVIDIN BIOTIN IN THE COMMON CARP CARRIERS) Raden Wasito; Hastari Wuryastuti; Bambang Sutrisno
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.433 KB)

In managing the koi herpesvirus (KHV) outbreaks as a routine national program in Indonesia, testingbased on biotechnology, such as immunopathologic immunohistochemical approach(es) using antibodythat is safe, rapid and accurate need to be applied. This will hopely assist the Government of Indonesianin improving and enhancing the sustainability of national animal proteins program. The present studywas aimed to develop and apply the immunopathologic immunohistochemistry of streptavidin biotin (IHCSB) for detection of KHV in the apparently normal carps. The gills from 48 common carps (Cyprinuscarpio) that appear to be healthy were prepared for DNA-based KHV by IHC SB. Common carps werecollected from fish farms which had an outbreak of KHV in 2008-2009 in Yogyakarta. All fish werenecropsied. The gills were processed histopathologically and then stained for IHC SB with monoclonalantibody anti-KHV. We demonstrated that all of the fish gills were positive for KHV antigen. Thus, it isconcluded that method is useful and consistent, very sensitive and rapid, and is a reliable method to beapplied for field condition to detect antigen KHV in the gills of normal, healthy looking carps. In addition,and more importantly, the fish can act as a source of KHV (carriers for KHV) and may result in the spreadof diseases among susceptible fish.

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