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I N. S. Sutama
Fakultas Peternakan UNUD
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PEROMBAKAN LIMBAH TANAMAN PANGAN OLEH BAKTERI SELULOLITIK ASAL CACING TANAH Suberata I W.; I M. Mudita; I N. S. Sutama
Jurnal Peternakan Tropika Vol 5 No 3 (2017)
Publisher : Animal Science Study Program, Faculty of Animal Husbandry, Udayana University

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Evaluation of ability on degrading agricultural waste by eight cellulolytic bacteria isolated from earthworm (Lumbricus rubellus) had been carried out for have the best cellulolytic bacteria as weeds crops degrader. The experiment was conducted with completely randomized design (CRD) 8 treatments and 3 replications, treatments based on the eight cellulolytic bacteria isolates with the code EB1CL, EB2CL, EB3CL, EB4CL, EB5CL, EB6CL, EB7CL and EB8CL.Evaluated of degrade ability measure by diameters of clear zone on agricultural waste were rice straw, rice bran, water hyacinth and water lettuce leaves. The results showed that cellulolytic bacteria isolated from earthworm coded EB1CL has produce highest clear zone diameters (P <0.05) on rice bran and water lettuce leaves substrate of 1,351 cm (Vs 1,239 - 1,331 cm) and 1,727 cm (Vs 1,621 - 1,721 cm), whereas the bacteria isolate coded EB8CL has produce highest clear zone diameter (P <0.05) on rice straw and water hyacinth substrates that were 1.060 cm (vs. 1.010 - 1.053 cm) and 1.495 cm (vs 1.394 - 1.494 cm). The bacteria isolates coded EB2CL had the lowest degradation ability on all substrates measured. Based on the result of this research, it can be concluded that cellulolytic bacteria isolate from earthworm with code EB1CL and EB8CL have the best ability in degrading of agricultural waste. Key words: Cellulolytic Bacteria, Earthworm, Ability on degrading substrates, Agricultural Waste
AKTIVITAS ENZIM LIGNOSELULASE INOKULAN YANG DIPRODUKSI DARI BERBAGAI TINGKAT PENGGUNAAN CACING TANAH (Lumbricus rubellus) Kusumajaya K. D.; I M. Mudita; I N. S. Sutama
Jurnal Peternakan Tropika Vol 4 No 2 (2016)
Publisher : Animal Science Study Program, Faculty of Animal Husbandry, Udayana University

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This study aimed to evaluate the effect of the use of earthworms to the activity of the enzyme lignoselulase of inoculants and best use of earthworms levels capable of producing an inoculant with the highest enzyme activity. This research was conducted at the Laboratory of Animal Nutrition and Feed Faculty of Animal Husbandry Universitas Udayana for 3 months. The Research using a completely randomized design (CRD) 4 treatments and 3 replications. Treatments were inoculant are produced using 0.1% of earthworm (BC1), inoculant are produced using 0.2% of earthworm (BC2), inoculant are produced using 0.3% of earthworm (BC3) and inoculant are produced using 0.4% earthworm (BC4). The variables were observed in this study was the enzyme activity of lignase, cellulase and xylanase in four periods of 30 minutes, 1 hour, 2 hours and 3 hours of incubation. The results showed that the increased use of earthworms from 0.1% to 0.2 to 0.4% in the production of inoculant can increase enzyme activity lignase, cellulase, and xylanase in each period of time incubation. The use of 0.4% earthworm level (BC4) was able to produce highest and significantly different (P
PENGARUH PENGGUNAAN BIOSUPLEMEN BAKTERI UNGGUL ASAL RAYAP TERHADAP ORGAN DALAM ITIK BALI JANTAN Dwipayana G. I. R.; G. A. M. K. Dewi; I N. S. Sutama; I M. Mudita
Jurnal Peternakan Tropika Vol 5 No 1 (2017)
Publisher : Animal Science Study Program, Faculty of Animal Husbandry, Udayana University

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Abstract

This study aimed to determine the effect  of using superior termites bacteria biosupplement in ration on the internal organ of bali drakes up to 12 weeks of age. this experiment used of 75 birds with homogenuous body weight ( 223,8 ± 11,19 g). The experimental design used in this study was complettely randomized design (CRD) with 5 treatments and 3  replicated those of  RB, RBio0, RBio1, RBio2, RBio1-2 treatments were duck fed basal ration, fed basal ration with biosupplement without superior termites bacteria, fed basal ration with biosupplement containing superior 1 termites bacteria, fed basal ration with biosupplement containing superior 2 termites bacteria, fed basal ration with biosupplement containing combination superior 1 and 2 termites bacteria respectively. The variables were observed were the heart weight, liver weight, bile weight and spleen weight. The results of this study showed that fed biosupplement containing combination superior for variable of heart weight, liver weight, bile and spleen weight, fed biosupplement superior bacteria termites in basal ration were not significant different (P>0,05).
KEMAMPUAN DEGRADASI DARI ISOLAT BAKTERI LIGNOLITIK ASAL CACING TANAH (Lumbricus rubellus) PADA SUBSTRAT GULMA TANAMAN PANGAN Marbun J. Y. F.; I N. S. Sutama; I M. Mudita; I W. Wijana
Jurnal Peternakan Tropika Vol 4 No 3 (2016)
Publisher : Animal Science Study Program, Faculty of Animal Husbandry, Udayana University

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A research aiming to identify and evaluate lignin degradation capability of lignolytic bacteria isolate isolated from earthworms on weeds crops substrates have been done in Nutrition and Forage Laboratory of Animal Husbandary Faculty, Udayana University for 3 (three) months. Evaluation on the degradation capability of lignolytic substrate was based on clear zone diameter that was formed in the substrates of water hyacinth, and water lettuce leaves. The research was based on Completely Randomized Design (CRD) with 3 (three) treatments and 3 (three) repetitions. The treatments are lignolytic bacteria isolates with codes EB1LG, EB2LG and EB3LG. Research result showed that on the water hyacinth and water lettuce leave substrates, showed that bacteria isolate coded EB1LG has highest clear zone diameters (1,525 cm and 1,528 cm) were 1,05% and 0,64% or 0,15% and 1,32% higher than bacteria isolates coded EB2LG and EB3LG, yet statistically it was not significantly different (P>0.05). Based on the research it can be concluded that on all substrates, namely water hyacinth, and water lettuce leaves, the three lignolytic bacteria isolated from earthworms have almost identical capability in degrading lignin.
Co-Authors Dwipayana G. I. R. G. A. M. K. Dewi I M. Mudita I W. Wijana I WAYAN SUBERATA Kusumajaya K. D. Marbun J. Y. F.