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All Journal Jurnal MIPA PHARMACON Jurnal Bios Logos CHEMISTRY PROGRESS JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI
Febby Ester Fany Kandou
Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Mathematics Medicine & Pharmacology Physics

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Journal : CHEMISTRY PROGRESS

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ANALISIS MOLEKULER ESCHERICHIA COLI SEROTYPE O157:H7 PADA AIR MINUM DALAM KEMASAN DAN ISI ULANG MENGGUNAKAN TEKNIK Kandou, Febby Ester Fany
CHEMISTRY PROGRESS Vol 2, No 1 (2009)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.2.1.2009.57

Abstract

This research aims to analyze the existence of Escherichia coli serotype O157:H7 in packaged andrefilled drinking water using Polymerase Chain Reaction (PCR) technique with gene target rfbE. Theresearch was carried out at Molecular Biology and Immunology Laboratory of Medical Faculty,Hasanuddin University Makassar. The methode used was Polymerase Chain Reaction (PCR) techniquei.e. Isolation/Extraction DNA bacteria of Escherichia coli serotype O157:H7 with Boom methode,Amplification DNA fragments of bacteria and Electrophoresis for visualization DNA fragments. Theresults show that based on DNA band that resulted, there are 8,33% of packaged drinking watersamples and 25% of refilled drinking water samples are contaminated by Escherichia coli serotypeO157:H7.
Co-Authors Alkawi, Alkawi Deidy Yulius Katili Husain, Rini Marina F. O. Singkoh Pelealu, Johanis Jullian Pience Veralyn Maabuat Putri, Ni Made Marlin Suarjo Regina R. Butarbutar Rondonuwu, Sendy Beatrix Rori, Chindy Achika Tangapo, Agustina Monalisa