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Antibacterial Activity of Semi Purified Extract of Marine-Derived Trichoderma reesei PDSP 5.7 Using Bioguided Fractionation Method Mada Triandala Sibero; Nunung Febriany Sitepu; Tao Zhou; Yasuhiro Igarashi
Buletin Oseanografi Marina Vol 9, No 1 (2020): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (652.778 KB) | DOI: 10.14710/buloma.v9i1.29192

Abstract

Bioguided fractionation method is commonly used to obtained targeted fraction with certain biological activity. In this study, bioguided fractionation method using HPLC was applied to obtained antibacterial fractions from marine-derived fungus Trichoderma reesei PDSP 5.7. The result shows that fraction from mycelium and medium extract had antibacterial activity against ESBL E. coli and S. enterica ser. Typhi with a range of inhibition zone was 1.35±0.15 to 8.82±0.22 mm2. From 32 fractions of each extract, the mycelial extract had 7 active fractions, 3 active fractions from broth medium which extracted using 1-butanol and 5 active fractions from ethyl acetate extract. This study indicated that crude extract of fungus T. reesei PDSP 5.7 was more potential as the source of antibacterial agents rather than the crude extract that obtained from its broth medium.
Training and Assistance for Housewives' Group to Create a CEHATI Family (Smart, Healthy, and Active) in Efforts to Maintain Children's Health Lufthiani Anwar; Siti Zahara Nasution; Cholina Trisa Siregar; Nunung Febriany Sitepu
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 1 (2022): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1799.792 KB) | DOI: 10.32734/abdimastalenta.v7i1.7258

Abstract

The school age is a critical period, because at this time infectious diseases often occur in children. The incidence of infectious diseases in children is increasing which will have an impact on the growth and physical development of children. The role of mother is very important such as in the implementation of health services for children and families in order to make efforts to prevent infectious diseases in children. The purpose of training and mentoring activities by forming a CEHATI (Smart, Healthy and Active) family can increase the knowledge and skills of mothers in efforts to maintain children's health at home. The method of implementing community service activities is carried out by providing education, training and assistance to 50 housewives groups. Education is carried out by providing health education about disease problems in children, PHBS and Handling the Risk of Injury to children, followed by simulation and training on handling injuries at home and providing assistance. The results of the implementation of this community service activity showed that the mother's knowledge of infectious diseases and PHBS was in good category (100%), after training and mentoring activities were carried out. The results of the paired t-test before and after being given training and mentoring obtained a p value of 0.000. As a result of this activity, it is hoped that the group of housewives can increase their knowledge and be independent in efforts to maintain children's health so that they are formed into a CEHATI (Smart, Healthy and Active) family.
MANAJEMEN BEREAVEMENT LIFE REVIEW TERHADAP SPIRITUAL WELLBEING PADA PASIEN POST STROKE DI RUMAH SAKIT Nunung Febriany Sitepu; Niskarto Zendrato; Dudut Tanjung; Lufthiani Lufthiani
Contagion: Scientific Periodical Journal of Public Health and Coastal Health Vol 4, No 1 (2022): CONTAGION
Publisher : Universitas Islam Negeri Sumatera Utara, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30829/contagion.v4i1.11599

Abstract

Spiritualitas merupakan factor protektif dalam proses berduka pada keluarga pasien kronis pada pasien post stroke. Bereavement life review  adalah salah satu intervensi dalam penguatan spiritual keluarga pasien penyakit post stroke. Bereavement life review merupakan intervensi yang prosesnya adalah dengan mencari dan menggali makna hidup individu sehingga makna spiritualitas pasien atau keluarga dapat meningkat. Bereavement life review njuga merupakan intervensi yang mudah, mcepat, dan dapat dilakukan oleh perawat yang terlatih. Tujuan penelitian ini adalah mengetahui pengaruh bereavement life review terhadap spiritual pasien post stroke. Desain penelitian ini adalah quasi-eksperimental dengan pretest posttest control  group. Sampel yang digunakan adalah salah satu pasien post  stroke yang menjalani fisiotherapy di rumah sakit. Sehingga didapatkan sampel sebanyak 30 responden dengan 15 kelompok kontrol dan 15 kelompok intervensi. Pengambilan sampel dilakukan dengan menggunakan consecutive sampling. Kelompok intervensi mendapatkan  bereavement life review dengan dua sesi yang dilakukan oleh perawat. Kesejahteraan spiritual  diukur menggunakan instrumen SWBS (spiritual well-being scale). Analisis data menggunakan dependent t-test, Mann Whitney dan Wilcoxon. Hasil penelitian menunjukkan adanya perbedaan skor rerata postest kesejahteraan spiritual pada kelompok kontrol dengan kelompok intervensi (98,71 ± 3,65 dan  106,5 ± 1,83; p = 0,000). Terdapat perbedaan skor rerata kesejahteraan spiritual pada pretest dengan posttest pada kelompok intervensi (99,07 ± 2,95 dan 106,5 ± 1,83; p = 0,001). Proses bereavement life review merupakan proses peningkatan spiritual melalui proses rekontekstualisasi, memaafkan terhadap diri, dan refleksi yang membentuk penguatan koping sehingga muncul pemaknaan  terhadap diri sendiri. Dapat disimpulkan bereavement life review berpengaruh positif terhadap peningkatan kesejahteraan spiritual keluarga pasien stroke. Bereavement life review  dapat digunakan sebagai intervensi perawatan pasien stroke dan keluarga.