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Deteksi Kemurnian Air Zamzam Menggunakan Metode Spektrofotometri Fourier Transform Infrared (FTIR) dan Kemometrik (Detection of Zamzam Water Purity Using Fourier Transform Infrared (FTIR) Spectroscopy Technique and Chemometrics) Kun Rasyida; Bambang Kuswandi; Nia Kristiningrum
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Zamzam water is the holy water located in the city centre of Mecca. This water is believed to have lots of benefits so that it consumed by moslems around the world. In 2010, saudi authorities implement strict rules regarding export of zamzam water. The rules led to the increase sale of fake zamzam water globally. The determination of zamzam water purity was develoved using FTIR spectroscopy combined with multivariate statistical methods (chemometrics). Zamzam water sample simulation consist of pure zamzam water, mineral water, and a mixture of zamzam water with mineral water in the concentration range of 10%-100%. Analysis of data using PLS, LDA, SIMCA, and SVM were based on two sets of data, in the whole absorption (4000-700 cm-1) and the absorption 1800-700 cm-1. The classification model LDA of data on the whole spectrum without preliminary treatment is the best chemometric classification model with recognition capabilitiy and ability of prediction of 100%, respectively. FTIR spectroscopy and chemometric methods have been sucessfully applied to detect the purity of zamzam water samples of circulating in Jember. Keywords: zamzam water, FTIR spectroscopy, chemometrics, training set, test set.

Pengembangan Chip Kertas untuk Deteksi Chronic Kidney Disease secara Dini (Development of Paper Based Chip for The Early Detection of Chronic Kidney Disease) Dwi Citra Nur Utami; Bambang Kuswandi; Lestyo Wulandari
Pustaka Kesehatan Vol 5 No 1 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i1.3953

Chronic kidney disease (CKD) is a condition of kidney which cannot perform its function normally, and expected to increase every year. Paper based chip was used in this case made by immobilized picric acid for creatinine detection, tetrabromophenol blue for protein detection, and bromothymol blue and methyl red for pH detection, on “whatman” filter paper which had been patterned using rubber based ink. The chip was applied for urine of patient, that has analitycal characteristics included response time approximately for 4 hours, linierity of creatinin achieved in concentration range of 0.1 -100 µg/ml and protein of 1 – 200 µg/ml, LOD (limit of detection) and LOQ (limit of quantification) of creatinin 0.088 µg/ml and 0.293 µg/ml, and protein 0.462 µg/ml and 1.540 µg/ml respectively. Interference study showed that urea, salt and sugar, interferen around 0.271-1.966% for creatinin and protein of 0.429 – 1.786%, respectively. The chip could be used up to 3 weeks if it stored in a dry place on room temperature and can be used to analyze condition of CKD over health condition. Keyword: CKD creatinin, protein, pH, and sensor

Label Pintar untuk Pemonitoran Kesegaran Daging Ayam pada Kemasan Novialda Nitiyacassari; Bambang Kuswandi; Dian Agung Pangaribowo
Pustaka Kesehatan Vol 9 No 2 (2021): Volume 9 No.2, 2021
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v9i2.10974

Chicken meat is one of the commodities consumed by most Indonesian people and has a pH range of 5,5 – 6,0. The smart label has been applied as a chicken meat freshness detector. As dual indicator freshness, two pH indicators have been used, i.e., bromothymol blue (BTB) and methyl red (MR). The objective of this research was to determine chicken meat freshness using a smart label at room temperature. The color change of the smart label was examined by ImageJ software to determine freshness degree using the mean RGB value. The chicken meat was examined every 2 hours for pH and TVB analysis during the 24 hours storage at room temperature. The result showed that color indicator would change according to chicken meat freshness, BTB turned from yellow to blue (mean RGB 210,591 ± 0,376), and MR turned from red to yellow (mean RGB 208,146 ± 0,390). Chicken meat freshness at room temperature decreased as pH increase from 5,68 to 6,11 along with a color change of smart label. Furthermore, the color would change when 0.022 %N of TVB has been reached. Therefore, chicken meat freshness can be determined by using a smart label based on a dual indicator of bromothymol blue and methyl red at room temperature.

Pengembangan Sensor Kloramfenikol Berbasis Bovine Serum Albumin menggunakan Spektrofotometri UV (The Development of Chloramphenicol Sensor Based on Bovine Serum Albumin using Spectrophotometry UV) Sarah Aisha; Bambang Kuswandi; Dwi Koko Pratoko
Pustaka Kesehatan Vol 6 No 1 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i1.6609

Misuse of antibiotics has been discovered in shrimp exported from Indonesia, one of them is chloramphenicol, therefore it is necessary to develop methods for chloramphenicol detection. In this study, the chemical sensor for chloramphenicol detection has been developed. The sensor developed by immobilized bovine serum albumin (BSA) onto cellulose acetate membrane. The sensor has linear range of 6-16 μg/ml chloramphenicol with r 0.999 and limit of detection (LOD)-limit of quantification (LOQ) value of 0.74-2.21 μg/ml. The RSD value of repeatability and intermediet precision were 2.41% and 2.31%, where the recovery was 100.29%. Three of the five samples of tiger shrimp on the market containing chloramphenicol at level of 1.03; 2.48; and 3 μg/ml. The result also shown in good agreement with conventional spectrophotometry UV-Vis method. Keywords: shrimp, chloramphenicol, BSA, cellulose acetate

Pengembangan Biosensor Berbasis Kertas untuk Penentuan Aktivitas Antihiperlipidemia Pada Sampel Ekstrak Tanaman Vinda Aisya Vira; Bambang Kuswandi; Indah Purnama Sary
Pustaka Kesehatan Vol 9 No 2 (2021): Volume 9 No.2, 2021
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v9i2.19051

A biosensor is an alternative method to determine the antihyperlipidemic activity to be more simple, effective, and efficient in various herbal extracts. This study aimed to develop a biosensor with the principle of pancreatic lipase enzyme inhibition in paper microzone plates using Whatman filter paper for the matrix, 200 unit/mL of lipase enzyme solution, 1% extract, and 80 mM p-NPB substrate. This paper-based biosensor method shows results that fulfilled the several characterizations, that are the 30 min of the incubation time; linearity using 7 standards orlistat concentrations of 5-35 mg/mL with the regression equation of Y= 22.09585 + 2.170514X, correlation coefficient value (r)= 0.9996745, Vx0= 1.509906%, Xp= 1.572533 mg/mL; LOD and LOQ are 1,573 mg/mL and 4,718 mg/mL; precision with RSD value of 2.545%; accuracy with % recovery value of 99.222%; biosensor usage time after storage remained stable for 3 days at chiller temperatures and for 45 minutes at room temperature. The paper-based biosensor can be an alternative method for detecting antihyperlipidemic activity in plant extracts as same as UV-Vis spectrophotometry method.

Pengembangan Bromfenol Biru dan Bromtimol Biru pada Label Pintar Sensor Kematangan Buah Naga Merah (Hylocereus polyrhizus) (The Development of Bromophenol Blue and Bromothymol Blue on Ripeness Sensor Smart Label on Red Dragon Fruit (Hylocereus polyrhizu Raden Ayu Rifqa Zainatul Hurriyah; Bambang Kuswandi; Dwi Koko Pratoko
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i3.5890

The novel on-package color indicator label has been fabricated based on bromophenol blue and mixture between bromophenol blue and bromothymol blue, and tests have been conducted to assess the ripeness of red dragon fruit (Hylocereus polyrhizus). Bromophenol blue (BPB) and mixture between bromophenol blue and bromothymol blue (BTB) were immobilized onto acetate cellulose membrane via adsorption method. The BPB and mixture between BPB and BTB/acetate cellulose membrane as color indicator work based on pH decrease as the volatile organic compounds (e.g. acetic acid). They produced gradually in the package headspace during developing of red dragon fruit. Subsequently, the color of the indicator will change from blue to green for over-ripe indication, which can be visible visually. The results showed that the color indicator could be used to determine the state of ripeness of the red dragon at ambient condition (25±2oC) and chiller condition (4±2oC). The color change of the indicators reflects the pH of headspace of the red dragon packaging. Furthermore, it also in similar trends to the change of several parameters (soluble solids content, pH, texture, sensory evaluation and weight loss) that normally used to characterize the ripeness of red dragon, therefore the indicator can be used for real time visual monitoring of ripeness state of packaged red dragon fruit. Keywords: ripeness indicator, BPB, BTB, red dragon fruit, acetate cellulose membrane

Deteksi Gelatin Babi pada Sampel Permen Lunak Jelly Menggunakan Metode Fourier Transform Infra Red (FTIR) dan Kemometrik (Detection of Porcine Gelatin in Jelly Soft Candy Sample Using Fourier Transform Infra Red and Chemometrics) Annisa Rahmawati; Bambang Kuswandi; Yuni Retnaningtyas
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Gelatin is widely used in some food products as gelling agent, including jelly soft candy. Most of commercial gelatin is derived from pork skin. Islam prohibits their followers to consume any food products containing pig derivates, including porcine gelatin. Therefore, it is necessary to develop some rapid and reliable methods for detection of porcine gelatin in jelly soft candy. FTIR method combined with chemometrics were chosen because it is rapid, easy and reliable. The purpose of this study was to classify of porcine gelatin in jelly soft candy using FTIR method combined with multivariate statistical methods (chemometrics). The classification method of chemometrics used to test porcine gelatin in commercial product of jelly soft candy was succesful and also in a good assessment with Xematest Pork. Keywords: bovine gelatin, jelly soft candy, porcine gelatin, FTIR, chemometrics.

Pengembangan Time-Temperature Indicator Berbasis Ekstrak Kulit Manggis (Garcinia mangostana L.) sebagai Sensor Penurunan Kualitas Susu Sapi Akibat Kesalahan Suhu Penyimpanan (Development of Time-Temperature Indicator Based on Mangosteen Rind (Garcinia Yayan Ika Rachmawati; Bambang Kuswandi; Nia Kristiningrum
Pustaka Kesehatan Vol 4 No 3 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Anthocyanin is reddish purple pigment found abundantly in fruits, vegetables, and flowers. Time-temperature indicator (TTI) based on anthocyanin extracted from mangosteen rind was developed for monitoring quality of cow's milk, especially fresh milk stored at room temperature. TTI consists of two membranes, namely indicator membranes and acetic acid membranes. Extract of mangosteen rind and sodium hidroxide solution was also co-immobilized onto Whatman filter paper to make indicator membrane, while acetic acid solution was also separately immobilized onto filter paper obtain acetic acid membrane. Color changes as TTI's responses were monitored directly using both visual inspection and quantitative measurement via color image analysis using ImageJ software. The color changes were further correlated with cow's milk freshness parameters such as pH, smell, and number of microbial. Fresh cow's milk quality spoiled after 4 hours stored at room temperature and color changes of TTI to bright yellow occured after 4 hours of exposure at room temperature. The result shown that the TTI can be applied for monitoring quality of cow's milk stored in room temperature. Keywords: mangosteen rind, TTI, cow's milk quality

Penentuan Adulterasi Daging Babi pada Sampel Burger Sapi Menggunakan Metode NIR dan Kemometrik (Determination of Pork Adulteration in Beef Burger Samples Using NIR and Chemometrics Method) Ani Mubayinah; Bambang Kuswandi; Lestyo Wulandari
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Burger is one of the favorite processed meat products. The adulteration of pork in halal-labeled beef burger becoming problem for the Muslim community. Therefore, it is necessary to develop some rapid and reliable methods for detection of pork in beef burger samples. NIR method combined with chemometrics were choosen because those methods are rapid, easy and reliable. The purpose of this study was to determine of pork adulteration in beef burger samples using NIR method combined with multivariate statistical methods such as Linear Discriminant Analysis (LDA), Soft Independent of Class Analogies (SIMCA), and Support Vector Machines (SVM). The NIR method and the best classification models of chemometric were applied in the beef burger samples. The result of NIR and Chemometrics is compared to the pork strip test. The results shows that the NIR-chemometrics method in good agreement with pork strip test toward pork adulteration in the beef burger samples tested. Keywords: pork, beef burger, NIR, chemometrics, pork test

Pengembangan Sensor Kloramfenikol Berbasis Imobilisasi Bovine Serum Albumin (BSA) pada Selulosa Asetat dengan Metode Spektroflorometri (The Development of Chloramphenicol Sensor Based on Bovine Serum Albumin (BSA) Immobilization on Cellulose Acetate using Spectrofluorometry Method) Dhany Alghifari; Bambang Kuswandi; Dwi Koko Pratoko
Pustaka Kesehatan Vol 5 No 1 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i1.3947

The chloramphenicol usage in shrimp cultivation is an example of antibiotics abuse that have adverse effects for health. According to Indonesian Drug and Food Agency (BPOM) survey, it showed that from 14 samples of shrimp test, entirely contain residues of chloramphenicol. In this context, the sensor has been developed base on cellulose acetate membrane immobilized with bovine serum albumin (BSA). The chloramphenicol sensor has a linearity range of 2-12 μg/ml and the r value of -0.997. The value of limit of detection is 0.157 μg/ml and limit of quantification is 0.472 μg/ml. The RSD value of repeatability is 1.542 % and the value of intermediate precision is 1.058%. The average value of recovery as the parameter an accuracy test of 95.338 ± 0.636% and this sensor has a good selectivity tests to erythromycin, pellets and shrimp meat. The result also shown in good agreement with the conventional spectrofluorometry method Keywords: shrimp, chloramphenicol, BSA, sensor, spectrofluorometry

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