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Uji Aktivitas Antioksidan Kombinasi Daun Kopi Arabika (Coffea arabica) dan Daun Pandan (Pandanus amaryllifolius) dengan Metode DPPH Retnaningtyas, Yuni; Hamzah, Muhammad Hafidi; Kristiningrum, Nia
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 9, No 1 (2017): Punica Granatum
Publisher : Indonesian Research Gateway

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ABSTRACT : Antioxidants are nutrient that can prevent the oxidative damage in the body. Arabica coffee and pandan is most common species of plant found in Indonesia. Arabica coffee and pandan known as plants with antioxidant activity. This study aimed to determine and compare antioxidant activity of water and ether fraction from methanolic extract of Arabica coffee leaves, pandan leaves and both combination by DPPH method. Vitamin C was used as positive control. The result show that Arabica coffee leaves have antioxidant activity better than pandan leaves and also gives big donation of antioxidant activity in their combinations. Statistical analysis showed that antioxidant activity from all sample were significanly different (p<0,05).

Uji Aktivitas Antioksidan Kombinasi Daun Kopi Arabika (Coffea arabica) dan Daun Pandan (Pandanus amaryllifolius) dengan Metode DPPH Retnaningtyas, Yuni; Hamzah, Muhammad Hafidi; Kristiningrum, Nia
Jurnal Farmasi Indonesia Vol 9, No 1 (2017)
Publisher : Jurnal Farmasi Indonesia

ABSTRACT : Antioxidants are nutrient that can prevent the oxidative damage in the body. Arabica coffee and pandan is most common species of plant found in Indonesia. Arabica coffee and pandan known as plants with antioxidant activity. This study aimed to determine and compare antioxidant activity of water and ether fraction from methanolic extract of Arabica coffee leaves, pandan leaves and both combination by DPPH method. Vitamin C was used as positive control. The result show that Arabica coffee leaves have antioxidant activity better than pandan leaves and also gives big donation of antioxidant activity in their combinations. Statistical analysis showed that antioxidant activity from all sample were significanly different (p<0,05).

Uji Aktivitas Antioksidan Kombinasi Daun Kopi Arabika (Coffea arabica) dan Daun Pandan (Pandanus amaryllifolius) dengan Metode DPPH Retnaningtyas, Yuni; Hamzah, Muhammad Hafidi; Kristiningrum, Nia
Jurnal Farmasi Indonesia Vol 9, No 1 (2017)
Publisher : Jurnal Farmasi Indonesia

ABSTRACT : Antioxidants are nutrient that can prevent the oxidative damage in the body. Arabica coffee and pandan is most common species of plant found in Indonesia. Arabica coffee and pandan known as plants with antioxidant activity. This study aimed to determine and compare antioxidant activity of water and ether fraction from methanolic extract of Arabica coffee leaves, pandan leaves and both combination by DPPH method. Vitamin C was used as positive control. The result show that Arabica coffee leaves have antioxidant activity better than pandan leaves and also gives big donation of antioxidant activity in their combinations. Statistical analysis showed that antioxidant activity from all sample were significanly different (p<0,05).Â Â

Pelatihan Pembuatan Souvenir Sabun Kepada Kelompok Ibu-Ibu Rumah Tangga Kecamatan Kaliwates Kabupaten Jember Dalam Rangka Meningkatkan Perekonomian Masyarakat Dwi Koko Pratoko; Nia Kristiningrum; Adelia A Devi Devi; Regol S Raudiah Raudiah
Dedication : Jurnal Pengabdian Masyarakat Vol 3 No 2 (2019)
Publisher : LPPM IKIP Jember

Sasaran dari kegiatan pengabdian masyarakat ini adalah kelompok ibu-ibu rumah tangga di Kecamatan Kaliwates Jember. Metode pelaksanaan dari kegiatan ini dibagi menjadi beberapa tahap yaitu persiapan, pelaksanaan pelatihan pembuatan souvenir sabun dan pelatihan pembuatan toko daring. Peserta dibagi menjadi kelompok-kelompok kecil sehingga sangat efektif dalam meningkakan pemahaman dan keterampilan kerja peserta. Adanya kegiatan ini diharapkan dapat memberikan ide, pengetahuan dan keterampilan bagi kelompok ibu rumah tangga tersebut sehingga mempunyai rumah produksi souvenir sabun dan harapannya bisa meningkatkan perekonomian keluarganya.

Kadar Fenolat dan Flavonoid Total serta Kapasitas Antioksidan Ekstrak Etanol dan Fraksi Jahe Merah (Zingiber officinale var. Rubrum) Dwi Koko Pratoko; Firdha Aprillia Wardhani; Nia Kristiningrum; Fifteen Aprila Fajrin; Dian Agung Pangaribowo
Al-Kimia Vol 6 No 2 (2018): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v6i2.6316

Determination of total phenolic, total flavonoid, and antioxidant capacity of ethanolic extract and fractions from red ginger (Zingiber officinale var. Rubrum) has been done. Red gingers contain the highest oleoresins among other ginger varieties. Oleoresins are component that had the most responsibility for pharmacological effect of red ginger, one of them is as antioxidant. Phenolic and flavonoid are highly contributing to the antioxidant effect of red ginger. The aim of this research is to determine the antioxidant capacity of extract and fractions of red ginger, and to investigate the correlation between antioxidant capacity with both total phenolic and total flavonoid. Ethanolic extracts are fractinationed with n-hexane, chloroform, ethyl acetate, n-butanol, and methanol solvent. Antioxidant capacity, total phenolic, and total flavonoid of all the samples are measured. Highest antioxidant capacity, total phenolic, and total flavonoid obtained from ethyl acetate fraction with TEAC 2143.9 ± 0.9 µmol/g (CUPRAC) and 4526.4 ± 3.0 (DPPH), GAE 229.9 ± 1.3 mg/g, and QE 46,6 ± 1,8 mg/g. One way ANOVA and post hoc analysis show significant result with p value <0,01. Pearson correlation shows high positive correlation. Determination of total phenolic, total flavonoid, antioxidant capacity from ethanolic extract and fractions of red ginger (Zingiber officinale var. Rubrum) has been done.Red ginger contain the highest oleoresins among other ginger varieties. Oleoresins are component that had the most responsibility for pharmacological effect of red ginger, one of them is as antioxidant. Phenolic and flavonoid are highly contribute to the antioxidant effect of red ginger. The aim of this research is to determine the antioxidant capacity of extract and fractions of red ginger, and to investigate the correlation between antioxidant capacity with both total phenolic and total flavonoid. Ethanolic extracts are fractinationed with n-hexane, chloroform, ethyl acetate, n-butanol, and methanol solvent. Antioxidant capacity, total phenolic, and total flavonoid of all the samples are measured. Highest antioxidant capacity, total phenolic, and total flavonoid obtained from ethyl acetate fraction with TEAC 2143,893 ± 0,890 µmol/g, GAE 229,878 ± 1,330 mg/g, and QE 46,564 ± 1,804 mg/g. One way ANOVA and post hoc analysis show significant result with p value <0,01. Pearson correlation shows high positive correlation.

Deteksi Kemurnian Air Zamzam Menggunakan Metode Spektrofotometri Fourier Transform Infrared (FTIR) dan Kemometrik (Detection of Zamzam Water Purity Using Fourier Transform Infrared (FTIR) Spectroscopy Technique and Chemometrics) Kun Rasyida; Bambang Kuswandi; Nia Kristiningrum
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Zamzam water is the holy water located in the city centre of Mecca. This water is believed to have lots of benefits so that it consumed by moslems around the world. In 2010, saudi authorities implement strict rules regarding export of zamzam water. The rules led to the increase sale of fake zamzam water globally. The determination of zamzam water purity was develoved using FTIR spectroscopy combined with multivariate statistical methods (chemometrics). Zamzam water sample simulation consist of pure zamzam water, mineral water, and a mixture of zamzam water with mineral water in the concentration range of 10%-100%. Analysis of data using PLS, LDA, SIMCA, and SVM were based on two sets of data, in the whole absorption (4000-700 cm-1) and the absorption 1800-700 cm-1. The classification model LDA of data on the whole spectrum without preliminary treatment is the best chemometric classification model with recognition capabilitiy and ability of prediction of 100%, respectively. FTIR spectroscopy and chemometric methods have been sucessfully applied to detect the purity of zamzam water samples of circulating in Jember. Keywords: zamzam water, FTIR spectroscopy, chemometrics, training set, test set.

Penentuan Kadar Fenol Total pada Ekstrak Daun Tanaman Menggunakan Metode Spektroskopi NIR dan Kemometrik (Determination of Total Phenolic in Leave Extracts Using Spectroscopy NIR and Chemometric) Fracilia Arinda Ratnasari; Lestyo Wulandari; Nia Kristiningrum
Pustaka Kesehatan Vol 4 No 2 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Phenols are group which having one or more hydroxyl groups which attached in aromatic ring and are found in plants. The aim of this research was to study whether NIR and chemometric methods could be used to determine the total phenol content of leave extracts. These methods were compared to UV-Vis spectrophotometry. Phenol was extracted from plant leaves by ultrasonic and maceration. NIR spectral data of selected leave extracts were correlated with total phenol content using chemometric. In this study, the chemometric method that used for quantitative and qualitative analysis were Partial Least Square (PLS) and Linear Discriminant Analysis (LDA), respectively. The PLS R2 calibration was 0.9920369 and RMSEC was 2.0049472. In addition, the R2 of LOOCV and 2-Fold-Cross-Validation were 0.9939401 and 0.9834646, respectively. Furthermore, LDA gave accuracy of 100%. The significance of phenol total that have been measured by NIR and UV-Vis spectrophotometry was evaluated with paired samples T-test and gave no significant difference. In conclusion, total phenol content can be measured using NIR and chemometric methods. Keywords: phenol, chemometric, LDA, NIR, PLS

Validasi dan Penetapan Kadar Rhodamin B pada Lipstik yang Beredar di Sekitar Universitas Jember dengan Metode KLT-Densitometri (Validation TLC-densitometry method for determination of Rhodamine B in Lipstick at Jember University Area) Risa Wahyu Ananda; Nia Kristiningrum; Yuni Retnaningtyas
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Rhodamine B is one of the synthetic dyes that forbidden in cosmetics. The study was conducted to validate TLC-densitometry method for determination Rhodamin B in lipstick at Jember University area. Samples was taken by purposive sampling method. The analyte were dissolved with ethanol 70% and cromatographed on silica Gel GF 254 TLC plate using etil asetat : metanol : amoniak in the ratio (15:3:3) (v/v/v). Then, validation and determination sample can be done using TLC-densitometry with maximum wavelength 554 nm. Method was found linear over the concentration range of 6 – 32 ng with correlation coefficient of 0,9965. Spesificity showed calculation of purity and identity more than 0.99. The limits of detection and quantitation limits obtained 0.558 and 1.86 ng/spot. The precision test which includes precision repeatability and intermediate precision, amounting to RSD 6.57% and test accuracy , resulting % recovery and RSD value of 97.74 % ± 2.54% . Based on these results lipstick sample purposively sampled none containing rhodamine B and safe consumer use. Keywords: TLC - densitometry , lipstick , rhodamine B , the validation method of analysis

Penetapan Kadar Fenol Total dan Pengujian Aktivitas Antioksidan Minyak Jahe Gajah (Zingiber officinale var. officinale) (Determination of Total Phenolic Content and Antioxidant Activity of Jahe Gajah (Zingiber officinale var. officinale) Oil) Tsabit Barki; Nia Kristiningrum; Endah Puspitasari; Fifteen Aprila Fajrin
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i3.5897

Jahe gajah (Zingiber officinale var. officinale) is one of export commodity plant in Indonesia. Part of jahe gajah often to be used is the rhizome, that content lots of essentials oil. One of the essential oil content is a phenolic compound. Phenolic compound is a natural product function as antioxidant reducing oxidative damage in the body that cause inflammation. The aim of this study was to determine the total phenolic content and antioxidant activity of jahe gajah oil. The total phenol content was determined using Folin-Ciocalteu method and the antioxidant activity assay was done using DPPH method. The total phenol content for jahe gajah oil was 0.626 ± 0.022 mg GAE/g . The IC50 of jahe gajah oil was 5,766 ± 0,087 µl/ml. Antioxidant activity from jahe gajah oil was suggested to be contributed by the phenolic compound and result from synergistic action of all constituents. Keywords: essential oil, aktivitas antioxidant, phenolic content

Pengembangan Time-Temperature Indicator Berbasis Ekstrak Kulit Manggis (Garcinia mangostana L.) sebagai Sensor Penurunan Kualitas Susu Sapi Akibat Kesalahan Suhu Penyimpanan (Development of Time-Temperature Indicator Based on Mangosteen Rind (Garcinia Yayan Ika Rachmawati; Bambang Kuswandi; Nia Kristiningrum
Pustaka Kesehatan Vol 4 No 3 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Anthocyanin is reddish purple pigment found abundantly in fruits, vegetables, and flowers. Time-temperature indicator (TTI) based on anthocyanin extracted from mangosteen rind was developed for monitoring quality of cow's milk, especially fresh milk stored at room temperature. TTI consists of two membranes, namely indicator membranes and acetic acid membranes. Extract of mangosteen rind and sodium hidroxide solution was also co-immobilized onto Whatman filter paper to make indicator membrane, while acetic acid solution was also separately immobilized onto filter paper obtain acetic acid membrane. Color changes as TTI's responses were monitored directly using both visual inspection and quantitative measurement via color image analysis using ImageJ software. The color changes were further correlated with cow's milk freshness parameters such as pH, smell, and number of microbial. Fresh cow's milk quality spoiled after 4 hours stored at room temperature and color changes of TTI to bright yellow occured after 4 hours of exposure at room temperature. The result shown that the TTI can be applied for monitoring quality of cow's milk stored in room temperature. Keywords: mangosteen rind, TTI, cow's milk quality

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