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All Journal Jurnal Pengendalian Hayati Berkala Ilmiah Pertanian Biotropika Jurnal Proteksi Tanaman Tropis
Wagiyana Wagiyana
Fakultas Pertanian Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Environmental Science Immunology & microbiology

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POTENSI TEPUNG DAUN SIRSAK, SERAI, KENIKIR, JERUK NIPIS, DAN KLUWIH TERHADAP KUMBANG BUBUK KEDELAI (Callosobruchus analis F.) PADA KEDELAI (Glyxine max L.) DALAM SIMPANAN Laily Dwi Dzulhijja; Wagiyana Wagiyana; Sigit Prastowo
Berkala Ilmiah Pertanian Vol 2 No 1 (2019): FEBRUARI
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bip.v2i1.16167

Abstract

ABSTRACT This study aims to determine the effect of soursop leaf flour, lemongrass flour, kenikir leaf flour, lime leaf flour, and kluwih leaf flour on: 1) mortality, 2) damage to seeds, 3) shrinkage of seed weight, 4) appearance of adult C. analysts on soybean seeds in deposits and 5) laying eggs of C. imago female eggs, 6) probit analysis LT50. The experiment was carried out using a Completely Randomized Design (CRD). As treatments are: A) controls; B) soursop leaf flour in doses of 1gr, 2gr, and 3gr; C) Serai leaf flour with doses of 1gr, 2gr, and 3gr; D) kenikir leaf flour with doses of 1gr, 2gr, and 3gr; E) lime leaf flour with doses of 1gr, 2gr, and 3gr; and F) kluwih leaf flour with doses of 1gr, 2gr, and 3gr. Each treatment uses five replications. The observed parameters were analyzed by "F" test variance then if it was significantly different to find out the difference in the effect of treatment, an Honest Real Difference Test was conducted at a level of 5% distrust. The best mortality results using the treatment of soursop leaf flour dose 3gr on 5 days amounted to 1.80 tails; 1 month is 6.60; 2 months totaling 13.40; and 3 months totaling 11.20. The results of the observation of the number of eggs produced by C. C. the best analyst was the treatment of soursop leaf flour dose 3gr on 5 days amounting to 139 items, 1 month 119.20 items; 2 months 91.20 items; and 3 months 72.20 items. The best imago observation results were treatment of soursop leaf flour dose 3gr at 1 month amounting to 132.20 tails; while at 2 months there were 79 heads. The best observation of seed damage and shrinkage of seed weight was the treatment of soursop leaf flour with a dose of 3gr, in the damage of seeds of 67.40 grains of seed damage; while the seed weight is reduced by 17.94 gr. The results of the best analysis of Probit LT50 were treatment of soursop leaf flour with a dose of 3gr because it could kill 50% of the population C. Analysts with a relatively short time were 9 days after treatment. Keywords: C. analis, leaf flour, dosage ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh tepung daun sirsak, tepung daun serai, tepung daun kenikir, tepung daun jeruk nipis, dan tepung daun kluwih terhadap: 1) mortalitas, 2) kerusakan biji, 3) susut bobot biji, 4) kemunculan dewasa C. analis pada biji kedelai dalam simpanan dan 5) peletakan telur imago betina C. analis, 6) analisis probit LT50. Percobaan dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL). Sebagai perlakuan adalah: A) kontrol; B) tepung daun sirsak dengan dosis 1gr, 2gr, dan 3gr; C) tepung daun serai dengan dosis 1gr, 2gr, dan 3gr; D) tepung daun kenikir dengan dosis 1gr, 2gr, dan 3gr; E) tepung daun jeruk nipis dengan dosis 1gr, 2gr, dan 3gr; dan F) tepung daun kluwih dengan dosis 1gr, 2gr, dan 3gr. Tiap perlakuan menggunakan lima ulangan. Terhadap parameter yang diamati dilakukan analisis dengan sidik ragam uji “F” kemudian apabila berbeda nyata untuk mengetahui perbedaan pengaruh perlakuan dilakukan Uji Beda Nyata Jujur pada taraf ketidakpercayaan 5%. Hasil mortalitas terbaik menggunakan perlakuan tepung daun sirsak dosis 3gr pada 5 hari sejumlah 1,80 ekor; 1 bulan sejumlah 6,60 ekor; 2 bulan sejumlah 13,40 ekor; dan 3 bulan sejumlah 11,20 ekor. Hasil pengamatan jumlah telur yang dihasilkan imago C. analis terbaik adalah perlakuan tepung daun sirsak dosis 3gr pada 5 hari sejumlah 139 butir, 1 bulan 119,20 butir; 2 bulan 91,20 butir; dan 3 bulan 72,20 butir. Hasil pengamatan imago muncul terbaik adalah perlakuan tepung daun sirsak dosis 3gr pada 1 bulan sejumlah 132,20 ekor; sedangkan pada 2 bulan sejumlah 79 ekor. Hasil pengamatan kerusakan biji dan susut bobot biji terbaik adalah perlakuan tepung daun sirsak dosis 3gr, pada kerusakan biji sejumlah 67,40 butir kerusakan biji; sedangan susut bobot biji sejumlah 17,94 gr. Hasil Analisis Probit LT50 terbaik adalah perlakuan tepung daun sirsak dosis 3gr karena dapat mematikan 50% populasi C. analis dengan rentan waktu yang cukup singkat yaitu pada 9 hari setelah perlakuan. Kata kunci: C. analis, tepung daun, dosis
Viabilitas Nematoda Entomopatogen Steinernema spp. dalam Media Kombinasi Senyawa Humik - WAGIYANA; Didik SULISTYANTO; Sugeng WINARSO
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.328 KB)

Abstract

Its well known, That Entomopathogenic Nematods (EPNs) used as biological agentswhich effective to control pest of Spodoptera litura (Noctuidae: Lepidoptera).The aims of thisresearch were to find out the multifungsion product as well as a biofeltilizer and biopesticide whichsuitable for agriculture sustainability, secondly the materials were to mixed the humic compound andbiological agent Entomopathogenic Nematodes (EPNs). Combination of biopesticide with EPNs asactive ingredient and humic compound as biofertilizer to find out a good product combination whichindicated the highest viability and activity of EPNs Steinernema spp on humic compound, in orderapplication of these meterial more effective and efficient to prepare the soil fertility and pest problem.This research was done with inoculation of EPNs on the medium/culture e.c: phosphate solublebacteria Pseudomonas putida 27.4B, zeolit and the humic compound (liquid culture). Pathogenecitytest of EPNs were done after incubation on the humic compound to the larvae of S. litura.The result of this research showed that long time of viability EPNs only for fourth weeks on the humicculture at (4000 ppm). Actually the EPNs from this incubation could 100 % mortality of the S. lituralarvae after 72 hours inoculation. The infection rate of EPNs on the larvae S. litura was 30,3 tailsduring 24 hours incubation. The viability of EPNs Steinernema spp on the medium with 1000 ppmhumic compound was 20 % after 120 hours and after 4 weeks later all EPNs could not survive on thismedium. It seems, the medium containing zeolith, P. putida 27.4B and humic compound at 1000 ppmwas not suitable medium neither for nematods growth or larvae S. litura survival.
Keragaman arthropoda herbivora dan musuh alami pada tanaman padi lahan rawa di Rowopulo Kecamatan Gumukmas Kabupaten Jember Muflih Adnan; W Wagiyana
Jurnal Proteksi Tanaman Tropis Vol 1 No 1 (2020): January
Publisher : Department of Plant Protection, Faculty of Agriculture, Universitas Jember - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jptt.v1i1.15586

Abstract

Rowopulo is one of the swamps in Gumukmas, Jember Regency with its unique soil in the form of peat soil that floats on water and by the community used for rice cultivation, whose production is influenced by the presence of Plant Pests, which is dominated by the Herbivod Arthropods. The presence of herbivorous Arthropods in the swampland rice ecosystem can be suppressed by the presence of useful Arthropod populations such as predators, parasitoids, and pollinators. Related to this, a study was conducted to find out the diversity of Arthropods found in swampy rice plantations. The study began with a land survey, then the determination and marking of observation plots on the map and matching these locations using GPS (Global Positioning System). Observations were made on plots that were made based on the extent of rice plants that were still in one stretch and the same irrigation flow. Each plot was observed ten times at 7-day intervals from rice plants aged 14 days after 77 days after planting. Arthropod sampling using sweep net method. Catching arthropods are put into vials of 5 ml size and then identified up to family level, and the diversity, evenness, and wealth index of the species is calculated. The results of research on swamp rice plants contained 37 families. Ten families act as herbivorous Arthropods, 15 families as predators, six families as parasitoids, one family as pollinators, and five families as detritivores. The highest diversity index in plot 1 was 2.95, while the lowest in plot 2 was 2.70, with the medium category for all observation plots. The magnitude of the diversity index is due to the dominance of only a few orders, such as Orthoptera and Diptera; the highest species wealth index in plot 3 with a value of more than 4 in the high category; Evenness index of all observation plots has a value of less than 1 with uneven categories. Keywords: arthropods, diversity, natural enemies, swamp land
Potensi tepung daun sirsak, serai, kenikir, jeruk nipis, dan kluwih terhadap kumbang bubuk kedelai (Callosobruchus analis F.) pada kedelai (Glyxine max L.) dalam simpanan Laily Dwi Dzulhijja; Wagiyana W; Sigit Prastowo
Jurnal Proteksi Tanaman Tropis Vol 1 No 2 (2020): July
Publisher : Department of Plant Protection, Faculty of Agriculture, Universitas Jember - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jptt.v1i2.17918

Abstract

This study aims to determine the effect of soursop leaf flour, lemongrass flour, kenikir leaf flour, lime leaf flour, and kluwih leaf flour on: 1) mortality, 2) damage to seeds, 3) shrinkage of seed weight, 4) appearance of adult C. analysts on soybean seeds in deposits and 5) laying eggs of C. analis imago female eggs, 6) probit analysis LT50. The experiment was carried out using a Completely Randomized Design (CRD). As treatments are: A) controls; B) soursop leaf flour in doses of 1 g, 2 g, and 3 g; C) Serai leaf flour with doses of 1 g, 2 g, and 3 g; D) kenikir leaf flour with doses of 1 g, 2 g, and 3 g; E) lime leaf flour with doses of 1 g, 2 g, and 3 g; and F) kluwih leaf flour with doses of 1 g, 2 g, and 3 g. Each treatment uses five replications. The observed parameters were analyzed by "F" test variance then if it was significantly different to find out the difference in the effect of treatment, an Tukey HSD Test was conducted at a level of 5% distrust. The best mortality results using the treatment of soursop leaf flour dose 3 g on 5 days amounted to 1.80 tails; 1 month is 6.60; 2 months totaling 13.40; and 3 months totaling 11.20. The results of the observation of the number of eggs produced by C. analis the best analyst was the treatment of soursop leaf flour dose 3 g on 5 days amounting to 139 items, 1 month 119.20 items; 2 months 91.20 items; and 3 months 72.20 items. The best imago observation results were treatment of soursop leaf flour dose 3 g at 1 month amounting to 132.20 tails; while at 2 months there were 79 heads. The best observation of seed damage and shrinkage of seed weight was the treatment of soursop leaf flour with a dose of 3 g, in the damage of seeds of 67.40 grains of seed damage; while the seed weight is reduced by 17.94 g. The results of the best analysis of Probit LT50 were treatment of soursop leaf flour with a dose of 3 g because it could kill 50% of the population C. analis with a relatively short time were 9 days after treatment.
Compatibility of Neem (Azadirachta indica) and Beauveria bassiana for Control of Spodoptera exigua and the Theoretical Impact to the Agroecosystem Agung Sih Kurnianto; Santi Prastiwi; Nilasari Dewi; Ahmad Ilham Tanzil; Wildan Muhlison; Wagiyana Wagiyana
Biotropika: Journal of Tropical Biology Vol. 10 No. 2 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.02.01

Abstract

Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae) is the biggest threat to onion farming, especially in the vegetative phase. In severe attacks, this pest can cause yield losses of up to 100%. Compatibility is one way to increase the efficiency and effectiveness of pest control by using plant-based pesticides or biological agents. Neem Leaf Extract (Azadirachta indica, acronym=NLE) has an azadirachtin compound that can inhibit insect growth, reduce appetite, reproduction, and hatch eggs. The fungus Beauveria bassiana (acronym=Bb) can secrete chitinase, lipase, proteinase enzymes that are able to decompose insect cuticles. NLE was obtained through the extraction method of plant-based pesticides and stored at 4oC until the experiment time. The Bb used was a commercial B. bassiana inoculum in the flour form with a density of 4.5 x 1010 spores/gram (trade name = Natural BVR). To determine the advantages of compatibility, this study is held by a single toxicity test of Bb and NLE, and the combined toxicity test of both. This study used a completely randomized design (CRD) consisting of 6 treatments with 4 replications, each replication used 12 larvae (a total of 48 larvae). Combination toxicity has a higher toxicity than the single use of NLE and Bb. At the LC 95 level, the combination treatment of Bb and NLE was 1.06-1.15 times more toxic than the single treatment of Bb, and 6.87-7.79 times more toxic than the single treatment of NLE. NLE and Bb are considered to have high compatibility (strong synergistic with GI value <0.5). Theoretically, the compatibility of NLE plant-based pesticides and Bb is very promising to replace chemical pesticides that have long-term adverse effects on agroecosystems.
Co-Authors Agung Sih Kurnianto Ahmad Ilham Tanzil Didik SULISTYANTO Laily Dwi Dzulhijja Laily Dwi Dzulhijja Muflih Adnan Nilasari Dewi Santi Prastiwi Sigit PRASTOWO Sugeng Winarso Wildan Muhlison, Wildan