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Nastiti Wijayanti
Fakultas Biologi Universitas Gadjah Mada
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RT-PCR TEST FOR DETECTION OF DENGUE VIRUS-3 IN INTRATHORACALLY INFECTED Aedes aegypti Asyhar Tunissea; Dyah Widiastuti; Nastiti Wijayanti
Widyariset Vol 15, No 2 (2012): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (766.495 KB) | DOI: 10.14203/widyariset.15.2.2012.479–484

Abstract

Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected Aedes aegypty. Detection of Dengue viral RNA in mosquito thorax using Reverse Transcription Polymerase Chain Reaction (RT-PCR) assay is an alternatives method for Dengue vector surveillance. The study aimed to test RT-PCR technique to detection Dengue-3 in intrathoracally infected Ae. aegypti. The study design was experimental with descriptive analytic using mosquitoes of Dengue Virus-3 were used as infectious samples and non-infected adult Ae. aegypti mosquitoes were used as normal ones. The results shows the RT-PCR technique can detect Dengue-3 viral RNA in intrathoracally Ae. aegypti at 5th ,6th and 7th days incubation.
Uji Viabilitas Virus Dengue Serotipe 3 pada Beberapa Galur Sel (Cell-Line) Dadan Supardan; Jaka Widada; Tri Wibawa; Nastiti Wijayanti
Biota Vol 9 No 1 (2016)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (978.062 KB) | DOI: 10.20414/jb.v9i1.42

Abstract

Infeksi virus dengue (DENV) merupakan infeksi yang menjadi salah satu masalah kesehatan utama di dunia dan salah satu infeksi yang sangat berbahaya terutama dinegara tropis seperti Indonesia.Infeksi dengue disebabkan oleh 4 serotipe virus dengue (DENV-1, DENV-2, DENV-3, dan DENV-4). Virus dengue serotipe 3 (DENV-3) merupakan serotipe dengan karakteristik lebih virulen. Sampai saat ini belum tersedia vaksin dan obat untuk pencegahan dan pengobatan infeksi virus dengue, sehingga penelitian penemuan obat demam berdarah sangat penting dilakukan, akan tetapi pengujian virus dengue pada kultur sel atau cell-line masih banyak mengalami kendala. Salah satu kendala yang sering dijumpai peneliti dalam pengujian senyawa tertentu terhadap virus dengue yaitu virus tersebut tidak mampu berkembang secara optimal pada beberapa jenis kulturcell-line, sehingga dilakukan pengujian untuk mengetahui viabilitas virus dengue pada beberapa kultur cell-line. Penelitian ini bertujuan untuk mengetahui kulturcell line yang paling cocok digunakan untuk berbagai pengujian infeksi DENV-3.
RT-PCR TEST FOR DETECTION OF DENGUE VIRUS-3 IN INTRATHORACALLY INFECTED Aedes aegypti Asyhar Tunissea; Dyah Widiastuti; Nastiti Wijayanti
Widyariset Vol 15, No 2 (2012): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (766.495 KB) | DOI: 10.14203/widyariset.15.2.2012.479–484

Abstract

Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected Aedes aegypty. Detection of Dengue viral RNA in mosquito thorax using Reverse Transcription Polymerase Chain Reaction (RT-PCR) assay is an alternatives method for Dengue vector surveillance. The study aimed to test RT-PCR technique to detection Dengue-3 in intrathoracally infected Ae. aegypti. The study design was experimental with descriptive analytic using mosquitoes of Dengue Virus-3 were used as infectious samples and non-infected adult Ae. aegypti mosquitoes were used as normal ones. The results shows the RT-PCR technique can detect Dengue-3 viral RNA in intrathoracally Ae. aegypti at 5th ,6th and 7th days incubation.
Co-Authors Asyhar Tunissea Dadan Supardan Dyah Widiastuti Jaka Widada Tri Wibawa