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All Journal The Indonesian Biomedical Journal BIOMA : Jurnal Biologi Makassar Biota JURNAL ILMU KEFARMASIAN INDONESIA BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) Kaunia: Integration and Interconnection of Islam and Science Journal
Tri Wibawa
Department Of Microbiology, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta 55281
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Veterinary

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Interferon-g-Inducible Protein 10 for Diagnosis of Tuberculosis in Children Stefani Candra Firmanti; Rina Triasih; Tri Wibawa; Sofia Mubarika Haryana
The Indonesian Biomedical Journal Vol 12, No 1 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i1.973

Abstract

BACKGROUND: The diagnosis of tuberculosis (TB) in children is challenging by the absence of a practical gold standard. Interferon (IFN)-ginducible protein 10 (IP-10) is a chemokine that may serve as the leading candidate marker in child TB diagnosis. The aim of this study is to assess the diagnostic value of IP-10 in the diagnosis of TB in children.METHODS: We recruited eligible symptomatic and asymptomatic children aged <15 years actively by contact investigation and passively from inpatient and outpatient clinics in two hospitals, in Yogyakarta, Indonesia. We conducted clinical examination and chest X-ray in all eligible children. Sputum smear and the rapid molecular TB test were performed in children with TB symptoms. All participants underwent blood sampling for IFN-g Release Assay and IP-10 test.RESULTS: A total of 79 children were recruited to this study. Twelve children were with TB disease, 16 with latent TB infection (LTBI), 40 were TB-exposed only and 11 were non-TB. Children with evidence of TB infection either with TB disease or LTBI had higher levels of antigen-stimulated IP-10 compared to non-infected children, both TB exposed only and non-TB (p=0.000). A cut-off 408.74 pg/mL for antigen-stimulated IP-10 showed high diagnostic accuracy for diagnosis of TB infection (AUC: 0.97, 95% CI: 0.92-1.00, sensitivity: 92.3%, and specificity: 91.9%). However, the stimulated levels of IP-10 between children with TB disease and LTBI were not significantly different (p=0.268).CONCLUSION: IP-10 performed well to diagnose TB infection in children. However, it cannot be used to differentiate TB infection from TB disease.KEYWORDS: IFN-g, IP-10, latent TB, active TB, children
PENGHAMBATAN CYTHOPHATIC EFFECT (CPE) PADA SEL BHK-21 YANG TERINFEKSI VIRUS DENGUE SEROTIPE 4 (DENV-4) DENGAN PEMBERIAN EKSTRAK N-HEKSAN STREPTOMYCES SP. GMR22 Diani Mentari; Jaka Widada; Tri Wibawa; Nastiti Wijayanti
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 2 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i2.10292

Abstract

Negara tropis seperti Indonesia sangat berpotensi sebagai tempat berkembangnya berbagai penyakit menular berbahaya salah satunya Demam Berdarah Dengue (DBD) yang disebabkan oleh infeksi virus dengue. Variasi genetik pada serotipe virus dengue menyebabkan penyakit ini menjadi perhatian dunia medis karena menyebabkan kompleksitas respon imun yang berbeda. Vaksin Dengue (Denvaxia) diperkenalkan oleh WHO pada akhir tahun 2015, namun penggunaannya belum sepenuhnya efektif. Hal ini menyebabkan banyak peneliti berupaya untuk mencari senyawa bioaktif yang memiliki potensi untuk dikembangkan sebagai agen antiviral. Streptomyces sp. GMR22 diketahui menghasilkan senyawa bioaktif dengan spektrum yang luas. Ekstrak n-heksane Streptomyces sp. GMR22 memiliki nilai CC50 yang tidak terlalu tinggi dibandingkan dengan ekstrak Etil Asetat. Hal ini menyebabkan metabolit sekunder yang dihasilkan dapat digunakan untuk analisis lanjut seperti uji antivirus karena aman terhadap sel BHK-21 yang merupakan host virus dengue. Virus DENV-4 merupakan serotipe endemik di Asia Tenggara. Penelitian ini bertujuan untuk mengetahui aktivitas antivirus DENV-4 menggunakan ekstrak n-heksan Streptomyces sp. GMR22. Aktivitas antivirus dilakukan melalui pengamatan sel BHK-21. Hasil pengamatan morfologi sel BHK-21 terinfeksi virus DENV-4 menunjukkan bahwa pemberian ekstrak n-heksan Streptomyces sp. GMR22 mengurangi terbentuknya Cythophatic Effect (CPE). Namun penggunaan konsentrasi 40 µg/mL menyebabkan kematian pada sel BHK-21. Kata kunci:  antiviral, DENV-4, metabolit sekunder,  Streptomyces sp. GMR22, CPE
KARAKTERISASI BAKTERI PENDEGRADASI FENOL DAN PEMBENTUK BIOFILM DARI SUMBER ALAMI DAN ARTIFISIAL Arifah Khusnuryani; Erni Martani; Tri Wibawa; Jaka Widada
Kaunia: Integration and Interconnection Islam and Science Vol. 11 No. 1 (2015)
Publisher : Fakultas Sains dan Teknologi UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/kaunia.1080

Abstract

Fenol merupakan salah satu polutan air tanah yang memiliki sifat toksik bagi manusia maupun lingkungan. Salah satu teknologi yang dapat diaplikasikan untuk pengolahan limbah fenol adalah melalui bioremediasi dengan memanfaatkan aktivitas bakteri. Secara umum, bakteri di alam akan tumbuh dan membentuk biofilm. Bakteri pembentuk biofilm memiliki beberapa kelebihan, diantaranya mampu bertahan hidup dalam lingkungan yang kurang menguntungkan serta meningkatkan degradasi senyawa rekalsitran, karena bakteri akan saling berinteraksi  dan saling melengkapi proses metabolik yang ada. Penelitian ini ditujukan untuk karakterisasi dan identifikasi isolat bakteri pendegradasi fenol dan pembentuk biofilm yang diperoleh dari limbah cair rumah sakit dan industri tekstil serta dari tanah gambut. Karakter yang diamati meliputi karakter morfologi koloni dan sel, serta karakter biokimiawi. Hasil karakterisasi selanjutnya digunakan untuk identifikasi menggunakan analisis profile matching isolat terpilih berdasarkan Bergey’s Manual of Determinative Bacteriology. Hasil menunjukkan bahwa bakteri yang diperoleh dari limbah cair rumah sakit memiliki karakter yang mirip dengan Genus Micrococcus (isolat DL120), Genus Enterobacter (DOK135), dan Genus Bacillus (ATA6). Isolat TU3 dari limbah cair industri tekstil mirip dengan Genus Flavobacterium. Isolat HG1, SG3, dan HP3 yang diperoleh dari tanah gambut berturut-turut menunjukkan karakter yang mirip dengan Genus Alcaligenes, Arthrobacter, dan Rhodococcus.
Uji Viabilitas Virus Dengue Serotipe 3 pada Beberapa Galur Sel (Cell-Line) Dadan Supardan; Jaka Widada; Tri Wibawa; Nastiti Wijayanti
Biota Vol 9 No 1 (2016)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (978.062 KB) | DOI: 10.20414/jb.v9i1.42

Abstract

Infeksi virus dengue (DENV) merupakan infeksi yang menjadi salah satu masalah kesehatan utama di dunia dan salah satu infeksi yang sangat berbahaya terutama dinegara tropis seperti Indonesia.Infeksi dengue disebabkan oleh 4 serotipe virus dengue (DENV-1, DENV-2, DENV-3, dan DENV-4). Virus dengue serotipe 3 (DENV-3) merupakan serotipe dengan karakteristik lebih virulen. Sampai saat ini belum tersedia vaksin dan obat untuk pencegahan dan pengobatan infeksi virus dengue, sehingga penelitian penemuan obat demam berdarah sangat penting dilakukan, akan tetapi pengujian virus dengue pada kultur sel atau cell-line masih banyak mengalami kendala. Salah satu kendala yang sering dijumpai peneliti dalam pengujian senyawa tertentu terhadap virus dengue yaitu virus tersebut tidak mampu berkembang secara optimal pada beberapa jenis kulturcell-line, sehingga dilakukan pengujian untuk mengetahui viabilitas virus dengue pada beberapa kultur cell-line. Penelitian ini bertujuan untuk mengetahui kulturcell line yang paling cocok digunakan untuk berbagai pengujian infeksi DENV-3.
Dampak Potensial Perubahan Iklim Terhadap Dinamika Penularan Penyakit DBD Di Kota Mataram Nur Alvira Pascawati; Tri Baskoro Tunggul Satoto; Tri Wibawa; Roger Frutos; Sylvie Maguin
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 15 Nomor 1 Juni 2019
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1544.876 KB) | DOI: 10.22435/blb.v15i1.1510

Abstract

Mataram City is an endemic area of DHF because cases are always found in 3 consecutive years with the number of cases that fluctuated and tended to increase. DHF is related to climate factors because of vector life, pathogen, behavior and the physiology of the human body is influenced by climate. The impact of climate change on the dynamics of dengue transmission in the city of Mataram is very important to study because it can be used as a basis for knowing the pattern of the occurrence of dengue cases related to temperature, humidity, rainfall and wind speed. This study used a retrospective cohort design from BMKG secondary climate data and dengue cases at the Mataram City Health Office in the last 5 years (2013-2017). Data were analyzed based on monthly and annual patterns assuming normal data distribution to be carried out correlation and regression tests with α = 0.05. The results showed that climatic elements such as: humidity, rainfall, and temperature had a strong enough influence on the incidence of DHF, but the wind speed was not related to the incidence of DHF in the city of Mataram. The linear regression equation model found was DBD Case = -439.403 + 5.809 * humidity (R2 = 18.9%) assuming all linear regression was fulfilled.This model can be used to assist in efforts to mitigate dengue transmission through the determination of the timing of implementation of prevention and the provision of infrastructure facilities for the DHF treatment.
Potensi Ekstrak Etanol Daun Sirih Merah (Piper crocatum) Sebagai Antimikrobakterium FARIDA JULIANTINA RACHMAWATY; BARMAWI HISYAM; MARSETYAWAN HNE SOESATYO; TRI WIBAWA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 1 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1487.035 KB)

Abstract

: The incidence of tuberculosis in Indonesia is high. There is a tendency for some people to use herbs for the treatment of tuberculosis. This research aims to investigate the potency of antimycobacterium property of red betel Vine (Piper crocatum) ethanolic extract against Mycobacterium tuberculosis (M. tuberculosis). The red betel vine leaf was extracted using standard ethanol solvent. The extract was tested on Mycobacterium tuberculosis H3 7Rv. The experiment was conducted by using serial dilution method with 50%, 40%, 25%, 20%, 12.5%, 10%, and 6.25% red betel vine ethanolic extract in Middlebrook 7H9 medium and subseqently inoculated into Lowenstein Jensen (LJ) medium. The result was analyzed descriptively by observing the minimum bactericidal concentration (MBC). The experiment was repeated three times to obtain consistent result. There is no bacterial growth in LJ media at concentrations of 50%, 40%, 25%, 20%, 12.5%, and 10% of red betel vine ethanolic extract. The bacteria was able to grow only at the concentration of 6.25% red betel vine ethanolic extract. Thus the ethanolic extract of red betel vine (Piper crocatum) indicates an antimycobaterium activity against M. tuberculosis H37Rv. The MBC is found at the concentration of 10%.
Co-Authors Arifah Khusnuryani BARMAWI HISYAM Dadan Supardan Diani Mentari Erni Martani Farida Juliantina Rachmawaty JAKA WIDADA Jaka Widada Jaka Widada MARSETYAWAN HNE SOESATYO Nastiti Wijayanti Nastiti Wijayanti Pascawati, Nur Alvira Rina Triasih Roger Frutos Sofia Mubarika Haryana Stefani Candra Firmanti Sylvie Maguin Tri Baskoro Tunggul Satoto