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All Journal Jurnal Ilmu Lingkungan Jurnal Ilmu Lingkungan Jurnal Kimia Riset Jurnal Pengabdian Masyarakat Biologi dan Sains
Eli Hendrik Sanjaya
Jurusan Kimia, FMIPA Universitas Negeri Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Physics

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POTENSI TRIMIRISTIN BIJI PALA FAKFAK (Myristica argantea warb) DAN EKSTRAKSI KULIT BUAH NAGA MERAH SEBAGAI BAHAN ADITIF SABUN DALAM PENERAPAN KIMIA HIJAU: REVIEW ANALISIS Isma Yanti Vitarisma; Atikah Atikah; Yudhi Utomo; Eli Hendrik Sanjaya
Jurnal Ilmu Lingkungan Vol 16, No 1 (2022): Jurnal Ilmu Lingkungan
Publisher : Program Pascasarjana Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jil.16.1.p.87-99

Abstract

Fakfak nutmeg seeds contain 6% of essential oil and 11-34% of fat. Trimyristin contained in nutmeg seeds reaches 80%. The oil and fat content in the nutmeg seeds can be used as an ingredient for making soap, perfume and other industrial materials. Tools made of stainless steel can be used to distill the oil content in nutmeg seeds. Reflux system using ester which will then be purified using acetone and then tested again with gas chromatography to isolate and purify nutmeg essential oil. Extraction of anthocyanin pigments from red dragon fruit peel waste using maceration method using distilled water and 10% citric acid with a ratio of 1:6 which is a mixed solvent type. The test parameters used pH values and absorbance values were measured using spectrophotometry with the UV-Visible method to determine and analyze the brightness level of the anthocyanin red dye content. The results of the content of trimyristin which can be potential as a soap additive and can be tested for anti-bacterial and anti-fungal properties combined with the principle of green chemistry.
INSERSI GEN pncA KE DALAM PLASMID pGEM-T Eli Hendrik Sanjaya
Jurnal Kimia Riset Vol. 1 No. 2 (2016): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1926.647 KB) | DOI: 10.20473/jkr.v1i2.3092

Abstract

Abstract. Multidrug-resistant tuberculosis (MDR-TB) is among the most worrisome elements of the pandemic of  antibiotic resistance. As the first line drug, pyrazinamide is often used to treat TB desease so there are many case of TB resistant to pyrazinamide. The previous research show that pncA gene of isolate L20 MDR-TB have mutated T539C. That mutation propose as the cause of resistance M. tuberculosis to pyrazimanide at the genetic level. For make sure the resistance mechanism, we have to get the pure PZAse and crystalization so the 3D structure can be determined by X-ray defraction. The first step to get the pure PZAse is cloning the pncA gene to the plasmid. The aim of this research is to know that is the pncA gene can be cloned to pGEM-T plasmid. The prosedure for cloning the pncA gene to the pGEM-T plasmid is amplification, followed by insert the pncA gene to the pGEM-T plasmid, and transformation by a selection of blue and white colony. The last step are isolation plasmid recombinant (pGEM-T-pncA) followed by electrophoresis. The result of the research showed that pncA gene from isolate L20 was successfully cloned to pGEM-T plasmid. That was showed on blue and white colony and the result of isolation and electrophoresis pGEM-T-pncA. The electrophoregram showed that the length of pGEM-T-pncA from white colony is different with pGEM-T standart abaut 0,7 kb. It is similar with the length of pncA gene (0,72 kb). Keywords: kloning, pGEM-T, pncA gene, pyrazinamide (PZA).
Identification of Species and Fatty Acid Composition of Microalgae from Raas Beach Sendang Biru Malang and Their Potential as Biodiesel Feedstock Eli Hendrik Sanjaya; Alifah Novitasari; Parlan Parlan; Laurent Octaviana; Dermawan Afandy; Sitoresmi Prabaningtyas
Jurnal Kimia Riset Vol. 2 No. 1 (2017): Juni
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.015 KB) | DOI: 10.20473/jkr.v2i1.4449

Abstract

Biodiesel is one of the best renewable energy made from triglycerides. Microalgae has a high lipid content include triglycerides, so it can be used as raw material for biodiesel production. Malang has many of beaches which are potent to become sources of microalgae, several beaches are surrounding Sendang Biru. The aims of this research were to identify the species of microalgae from Raas Beach Sendang Biru, determine their yield of lipid and fatty acid composition, and analyse their potential to became feedstock of biodiesel production. This research consists of five stages: identification of microalgae, cultivation, harvesting, determination of the yield of microalgae lipid by soxhlet extraction method, and analysis of the fatty acid composition of lipid by GC-MS. The results showed that the species of microalgae are dominated by Euglena sp and small amount of Gloeocapsa. The lipid yield of dried microalgae is 7.5% and the fatty acids composition are dodecanoic acid (1.6%), tetradecanoic acid (1.3%), hexadecanoic acid (16.6%), 9,12-octadecadienoic acid (25.2%), 9-octadecenoic acid (29.8%), and octadecanoic acid (6.7%). Based on this results, it can be concluded  that the microalgaes from Raas Beach Sendang Biru are potential to become biodiesel feedstock but it still need optimization.
Penggunaan Karbon Aktif dari Biji Kelor dan Berbagai Biomassa Lainnya dalam Mengatasi Pencemaran Air : Analisis Review Jacky Anggara Nenohai; Zelen S. Minata; Burhanuddin Ronggopuro; Eli Hendrik Sanjaya; Yudhi Utomo
Jurnal Ilmu Lingkungan Vol 21, No 1 (2023): January 2023
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.21.1.29-35

Abstract

Tujuan penelitian ini adalah untuk mengetahui (1) penggunaan karbon aktif dari berbagai bahan alami dalam penjernihan air dan (2) penggunaan biji kelor dalam penjernihan air, dan (3) cara menganalisis logam berat dari hasil penjernihan air. Metode yang digunakan dalam penelitian ini yaitu metode telaah pustaka yang sistematis, atau biasa disebut dengan Systematic Literature Review (SLR) yang dimana hasilnya menggunakan database artikel online yaitu Google Schoolar. Hasil telaah dari artikel yang berkaitan dengan topik diperoleh bahwa penggunaan karbon aktif atau arang yang terbuat dari bahan alami seperti kayu kesambi, ampas tebu, tongkol jagung, kulit buah durian dan eceng gondok sangat efektif dalam penyerapan zat terlarut air, baik organik maupun anorganik dalam menghasilkan air yang jernih. Selain itu, penggunaan biji kelor juga sangat efektif sebagai biokoagulan dan bioflokulan dalam menurunkan pH, kesadahan, kekeruhan dan total padatan tersuspensi, sehingga memungkinkan munculnya rekomendasi bahwa air hasil penjernihan dengan menggunakan karbon aktif dari berbagai bahan alami dan serbuk biji kelor dapat dijadikan air minum.
Pengaruh Sosialisasi Bahaya dan Cara Pengelolaan Limbah Batik Terhadap Tingkat Pemahaman Pembatik Desa Sumberejo Evi Susanti; Eli Hendrik Sanjaya; Retno Wulandari; Muh Ade Artasasta; Zarin Nafasari; Muhammad Reza Pahlevi; Sarif Hidayat; Shinta Yuliana
Jurnal Pengabdian Masyarakat Biologi dan Sains Vol 1, No 2 (2022): Jurnal Pengabdian Masyarakat Biologi dan Sains
Publisher : Universitas Indraprasta PGRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1005.938 KB) | DOI: 10.30998/jpmbio.v1i2.1472

Abstract

Desa Sumberejo merupakan salah satu desa binaan Universitas Negeri Malang yang berada di Kecamatan Gedangan Kabupaten Malang. Desa ini terdiri dari enam dusun yakni Dusun Sumbersari, Mulyosari, Krajan, Dokosari, Tlekung dan Sumberwangi. Desa Sumberejo dikenal sebagai desa penghasil batik, dengan branding “Batik Sujo”. Produksi Batik Sujo melibatkan ibu-ibu rumah tangga yang berasal dari berbagai dusun di Desa Sumberejo. UMKM ini memproduksi beberapa lembar kain batik dalam sehari dan setiap produksi menghasilkan limbah, khususnya limbah cair yang dapat menimbulkan pencemaran pada lingkungan. Edukasi mengenai dampak dan pengelolaan limbah cair batik perlu diketahui oleh semua pembatik, sehingga perlu dilakukan sosialisasi mengenai topik tersebut. Tujuan kegiatan ini untuk mengetahui dampak sosialisasi terhadap tingkat pemahaman mengenai bahaya limbah batik dan cara pengelolaannya. Metode penelitian menggunakan Quasi Experimental dengan One Group Pretest Posttest Design, dengan pengambilan subjek menggunakan metode Quota Sampling. Hasil penelitian menunjukkan bahwa sosialisasi berpengaruh terhadap Pembatik Sujo, karena terjadi peningkatan pemahaman mengenai dampak dan pengelolaan limbah batik meningkat 15%.
Co-Authors Alifah Novitasari Atikah Atikah Burhanuddin Ronggopuro Dermawan Afandy Evi Susanti Isma Yanti Vitarisma Jacky Anggara Nenohai Laurent Octaviana Muh Ade Artasasta Muhammad Reza Pahlevi Parlan Parlan Retno Wulandari Sarif Hidayat Shinta Yuliana Sitoresmi Prabaningtyas Utomo, Yudhi Zarin Nafasari Zelen S. Minata